Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Dkk-1 Antibody (B-7) - Western Blotting - Image 389391
Dkk-1 抗体 (B-7) HRP: sc-374574 HRP. 大鼠脑组织提取物中 Dkk-1 表达的直接 Western 印迹分析.
Dkk-1 抗体 (B-7) HRP: sc-374574 HRP. 大鼠脑组织提取物中 Dkk-1 表达的直接 Western 印迹分析.

Dkk-1 抗体 (B-7): sc-374574

4.3(12)
寫評論提問

说明书
  • Dkk-1 抗体 B-7 是小鼠单克隆 IgG1 κ，Dkk-1抗体, 在35篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Dkk-1的氨基酸1-120
  • Dkk-1 抗体 (B-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Dkk-1
  • 抗Dkk-1抗体（B-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dkk-1 (B-7): sc-374574的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Dkk-1 抗体 (B-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Dkk-1 抗体 (B-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Dkk 抗体 包括 Dkk-3

Dkk-1抗体（B-7）是一种IgG1 κ小鼠单克隆Dkk-1抗体（也称为Dickkopf WNT信号通路抑制剂1抗体，或富含半胱氨酸的分泌型Dickkopf家族成员1抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的Dkk-1蛋白。Dkk-1抗体（B-7）既有非偶联形式的抗Dkk-1抗体，也有多种偶联形式的抗Dkk-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Wnt基因是一组保守的、富含半胱氨酸的分泌型糖蛋白，对于许多发育过程，包括胚胎发育、不对称细胞分裂和中枢神经系统（CNS）模式是必需的。Wnt与七次跨膜受体Frizzled结合后激活Dishevelled，后者通过丝氨酸磷酸化下调糖原合成酶激酶（GSK），导致β-连环蛋白的积累和随后对发育有重要意义的Wnt靶基因的调控。Dickkopf家族作为Wnt信号的分泌型抑制剂，通过拮抗Wnt级联的不同阶段确保正确的形态发育。Dkk-1（Dickkopf-1）作用于β-连环蛋白和Dishevelled的上游，抑制Wnt信号。Dkk-1是一种由266个氨基酸（人类）组成的分泌型蛋白，包含一个由31个残基组成的N端信号肽、2个富含半胱氨酸的结构域和一个假定的C端N-糖基化位点。人类Dkk-1转录本在胎儿肾脏、成人胎盘和成人前列腺中大量存在。Dkk-1起始位点上游的假定顺式调控元件包括p53、Sp1、MyoD、STAT、Oct-1/2、C/EBP-α、C/EBP-β、GATA-1、GATA-2和GATA-3。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dkk-1 抗体 (B-7) 参考文献:

  1. 哺乳动物 Wnt 信号转导的新型阻抑剂--人类 Dkk-1 同源物的分离与生化特征。  |  Fedi, P., et al. 1999. J Biol Chem. 274: 19465-72. PMID: 10383463
  2. Dickkopf-1 是 Wnt 信号通路的抑制剂，由 p53 诱导。  |  Wang, J., et al. 2000. Oncogene. 19: 1843-8. PMID: 10777218
  3. Dkk-1-TNF-α crosstalk regulates MC3T3E1 pre-osteoblast proliferation and differentiation under mechanical stress through the ERK signaling pathway.  |  Wu, Y., et al. 2023. Mol Cell Biochem. 478: 2191-2206. PMID: 36640256
  4. 强直性脊柱炎血清DKK-1水平：荟萃分析和孟德尔随机化的见解。  |  Fang, X., et al. 2023. Front Immunol. 14: 1193357. PMID: 37503346
  5. DNA methylation of DKK-1 may correlate with pathological bone formation in ankylosing spondylitis.  |  Zou, YC., et al. 2023. Immun Inflamm Dis. 11: e911. PMID: 37506134
  6. DKK-1及其对骨破坏的影响：胶原诱导性关节炎小鼠和类风湿关节炎患者的比较研究。  |  Zhao, D., et al. 2024. Inflammation. 47: 129-144. PMID: 37688661
  7. Dickkopf-1（DKK-1）对前列腺癌生长和骨转移的影响。  |  Yuan, S., et al. 2023. Cells. 12: PMID: 38067123
  8. SARS-CoV-2触发Dickkopf-1（Dkk-1）调节小鼠的T辅助细胞和肺部病理。  |  Wu, Y., et al. 2024. Genes Dis. 11: 101167. PMID: 38362044
  9. Dishevelled是果蝇褐飞虱信号通路的一个组成部分。  |  Krasnow, RE., et al. 1995. Development. 121: 4095-102. PMID: 8575310
  10. Wnt信号：动物发育的共同主题。  |  Cadigan, KM. and Nusse, R. 1997. Genes Dev. 11: 3286-305. PMID: 9407023
  11. Dickkopf-1 是一种新的分泌蛋白家族成员，具有诱导头部发育的功能。  |  Glinka, A., et al. 1998. Nature. 391: 357-62. PMID: 9450748
  12. 轴突蛋白连接 beta-catenin 和糖原合酶激酶-3beta，并抑制 beta-catenin 介导的转录。  |  Sakanaka, C., et al. 1998. Proc Natl Acad Sci U S A. 95: 3020-3. PMID: 9501208

订购信息

产品名称产品编号规格价格数量收藏夹

Dkk-1 抗体 (B-7)

sc-374574
200 µg/ml
RMB2377.00

Dkk-1 (B-7): m-IgGκ BP-HRP 套装

sc-522782
200 µg Ab, 40 µg BP
RMB2662.00

Dkk-1 (B-7): m-IgG1 BP-HRP 套装

sc-533525
200 µg Ab; 20 µg BP
RMB2662.00

Dkk-1 抗体 (B-7) AC

sc-374574 AC
500 µg/ml, 25% agarose
RMB3129.00

Dkk-1 抗体 (B-7) HRP

sc-374574 HRP
200 µg/ml
RMB2377.00

Dkk-1 抗体 (B-7) FITC

sc-374574 FITC
200 µg/ml
RMB2482.00

Dkk-1 抗体 (B-7) PE

sc-374574 PE
200 µg/ml
RMB2580.00

Dkk-1 抗体 (B-7) Alexa Fluor® 488

sc-374574 AF488
200 µg/ml
RMB2685.00

Dkk-1 抗体 (B-7) Alexa Fluor® 546

sc-374574 AF546
200 µg/ml
RMB2685.00

Dkk-1 抗体 (B-7) Alexa Fluor® 594

sc-374574 AF594
200 µg/ml
RMB2685.00

Dkk-1 抗体 (B-7) Alexa Fluor® 647

sc-374574 AF647
200 µg/ml
RMB2685.00

Dkk-1 抗体 (B-7) Alexa Fluor® 680

sc-374574 AF680
200 µg/ml
RMB2685.00

Dkk-1 抗体 (B-7) Alexa Fluor® 790

sc-374574 AF790
200 µg/ml
RMB2685.00

能否用于小鼠脑组织切片（40 μm震荡切片）的免疫荧光？

Asked by: Anonymous
感谢您的提问。sc-374574 Dkk-1 (B-7)可以用于小鼠冰冻切片和石蜡切片的IF、IHC。 Thank you for your question. sc-374574 Dkk-1 (B-7) can be used for IF of frozen sections and paraffin sections in mouse.
Answered by: Zhanglong
Date published: 2024-10-17

抗体具有生物活性吗？就是结合相应靶点后，可以影响细胞通路，因为需要做细胞实验。

Asked by: 张 皓 宸
感谢您的提问。Dkk-1 抗体 (B-7): sc-374574推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Dkk-1。该抗体在其他实验中，没有进行过验证。 Thank you for your question. Dkk-1 Antibody (B-7): sc-374574 is recommended for detection of Dkk-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. This antibody was not tested in other applications.
Answered by: Technical Service SH
Date published: 2023-01-04

Buenas tardes, necesitamos determinar DKK1 mediante un inmunoensayo sobre un electrodo (inmunosensor electroquímico), para lo cual necesitaríamos un anticuerpo de captura, la interleucina DKK1 y un anticuerpo marcado, por ejemplo con Biotina o HRP.

Asked by: Anonymous
Gracias por su pregunta. Desafortunadamente, este anticuerpo no se ha probado con este ensayo en particular y no se recomienda para él. Ofrecemos este anticuerpo como una versión conjugada con HRP.
Answered by: Tech Support Europe
Date published: 2021-07-23

Can this antibody be used for FACS sorting?.

Asked by: ElenaR
Thank you for your question. We have not yet tested this mouse monoclonal, Dkk-1 Antibody (B-7): sc-374574, for FACS or FCM so this application is not recommended at this time. We do offer this antibody directly conjugated to phycoerythrin (sc-374574 PE), fluorescein (sc-374574 FITC), Alexa Fluor® 488 (sc-374574 AF488) or Alexa Fluor® 647 (sc-374574 AF647), 200 µg/ml. If you purchase one of these direct conjugates we will warranty the antibody for FCM.
Answered by: Technical Support
Date published: 2017-03-03

What is the total volume provided of Dkk-1 (B-7):sc-374574 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-13

Can Dkk-1 (B-7): sc-374574 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Dkk-1 (B-7): sc-374574 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-13
  • y_2025, m_9, d_6, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_zh_CN, sid_374574, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from 抗体特异性不错我两周前申请了小样，Dkk-1 抗体 (B-7),货号为sc-374574，鼠单抗，WB结果提示特异性不错
Date published: 2021-07-04
Rated 5 out of 5 by from Worked greatWorked great for immunofluorescence on paraffin-embedded murine samples.
Date published: 2020-10-06
Rated 4 out of 5 by from Good for WBIn MCF7 cells, a spcific band was found in WB, diluted at 1:1000.
Date published: 2018-04-12
Rated 5 out of 5 by from Great features！I applied for a free sample two weeks ago,then western blot detection dkk-1 of mouse. dilution:1:100 The experimental results are good!
Date published: 2017-09-18
Rated 4 out of 5 by from 需要进一步验证两个月前申请试用装，在皮肤中进行免疫荧光实验检测，做出了疑似阳性结果，还没有重复，希望能对大家有所帮助。
Date published: 2017-08-24
Rated 5 out of 5 by from Western BlotThis antibody is very clean in Western Blot with almost no additional bands, and those present are very faint.
Date published: 2017-04-15
Rated 5 out of 5 by from Outstanding productVery strong antibody for both western blot and IHC applications !!
Date published: 2017-01-20
Rated 5 out of 5 by from Good for IHCThis Dkk-1 antibody shows good results for IHC staining
Date published: 2017-01-12
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_374574, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
Dkk-1 抗体 (B-7) is rated 4.3 out of 5 by 12.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_374574, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。