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DJ-1 抗体 (mαDJ-1/E2-29): sc-33665

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  • DJ-1抗体(mαDJ-1/E2-29)是小鼠单克隆IgM κ, 在1篇文献中引用,规格为200 µg/ml
  • 免疫human物种的DJ-1
  • 推荐用于 human 来源的DJ-1 WB, IP 和 IF检测
  • 看 DJ-1 (D-4): sc-55572,可查看有标记的 DJ-1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是DJ-1 Antibody (mαDJ-1/E2-29) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与DJ-1 Antibody (mαDJ-1/E2-29) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。
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DJ-1抗体(mαDJ-1/E2-29)是一种小鼠单克隆IgM抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)等方法检测人类样本中的DJ-1。DJ-1是一种高度保守的蛋白质,在细胞过程中发挥着关键作用,特别是保护细胞免受氧化应激和维持线粒体功能。DJ-1通过与PIASxα形成复合物来调节雄激素受体,凸显其在各种信号通路中的重要性,这些信号通路会影响细胞的生长和存活。DJ-1主要位于细胞质中,但在细胞周期的S期会转移到细胞核中,表明它参与细胞周期的调节和对生长刺激的反应。DJ-1基因的突变与包括帕金森病在内的神经退行性疾病有关,这凸显了DJ-1在神经元健康中的重要性。编码人类DJ-1的基因位于1p36.23染色体上,该区域与多种肿瘤的染色体异常有关,进一步强调了DJ-1在癌症生物学中的重要性。抗DJ-1抗体(mαDJ-1/E2-29)是研究人员在研究DJ-1在健康和疾病中的多方面作用时的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DJ-1 抗体 (mαDJ-1/E2-29) 参考文献:

  1. 神经退行性疾病中DJ-1的表达。  |  Antipova, D. and Bandopadhyay, R. 2017. Adv Exp Med Biol. 1037: 25-43. PMID: 29147901
  2. DJ-1的转录调控。  |  Takahashi-Niki, K., et al. 2017. Adv Exp Med Biol. 1037: 89-95. PMID: 29147905
  3. DJ-1作为抗癌治疗靶点。  |  Cao, J., et al. 2017. Adv Exp Med Biol. 1037: 203-222. PMID: 29147911
  4. DJ-1通过与IP3R3-Grp75-VDAC1相互作用,调节ER-线粒体结合的完整性和功能。  |  Liu, Y., et al. 2019. Proc Natl Acad Sci U S A. 116: 25322-25328. PMID: 31767755
  5. DJ-1 通过保持 S-腺苷同型半胱氨酸水解酶的活性来抑制铁变态反应。  |  Cao, J., et al. 2020. Nat Commun. 11: 1251. PMID: 32144268
  6. DJ-1上调Nrf2/GPX4信号通路,抑制先兆子痫发病机制中的滋养细胞铁死亡。  |  Liao, T., et al. 2022. Sci Rep. 12: 2934. PMID: 35190654
  7. DJ-1 是 PINK1/parkin 依赖性有丝分裂的重要下游介质。  |  Imberechts, D., et al. 2022. Brain. 145: 4368-4384. PMID: 36039535
  8. DJ-1在自噬调节和疾病中的重要性。  |  Liu, S., et al. 2023. Arch Biochem Biophys. 743: 109672. PMID: 37336341
  9. DJ-1通过ATF4/HSPA5通路抑制脑缺血再灌注中的铁死亡。  |  Li, Y., et al. 2023. Neurochem Int. 171: 105628. PMID: 37820776
  10. DJ-1的N-高半胱氨酸化促进帕金森病的神经变性。  |  Guo, T., et al. 2024. Aging Cell. 23: e14124. PMID: 38380563

订购信息

产品名称产品编号规格价格数量收藏夹

DJ-1 抗体 (mαDJ-1/E2-29)

sc-33665
200 µg/ml
CNY2422.00

DJ-1 (mαDJ-1/E2-29): m-IgGκ BP-HRP 套装

sc-551929
200 µg Ab; 40 µg BP
CNY2715.00

Hi, What's the immunogen used to generate mαDJ-1/E2-29 antibody? Thank you

Asked by: Loukmane
Thank you for your question. The exact antigen sequence and epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-08-17

What is the recommended fixation method for immunofluorescence staining with DJ-1 (mαDJ-1/E2-29): sc-33665 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by TS from Clean blot with single strong band at expectedClean blot with single strong band at expected size using HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by CJ from Excellent immunofluorescence cytoplasmicExcellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by SM from Great antibody for WBGreat antibody for WB, works in A-431, Jurkat and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-27
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DJ-1 抗体 (mαDJ-1/E2-29) is rated 5.0 out of 5 by 3.
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