Date published: 2026-10-19

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DJ-1 Antibody (A-9) - Western Blotting - Image 379546
DJ-1 抗体 (A-9): sc-55573. HeLa (A), ZR-75-1 (B), U-698-M (C), HEL 92. 1. 7 (D) 和 K-562 (E) 全细胞裂解液中 DJ-1 表达的 Western 印迹分析.
DJ-1 抗体 (A-9): sc-55573. HeLa (A), ZR-75-1 (B), U-698-M (C), HEL 92. 1. 7 (D) 和 K-562 (E) 全细胞裂解液中 DJ-1 表达的 Western 印迹分析.

DJ-1 抗体 (A-9): sc-55573

4.9(8)
寫評論提問
说明书
  • DJ-1抗体(A-9)是小鼠单克隆IgG2a κ, 在7篇文献中引用，规格为200 µg/ml
  • 与氨基酸1-189结合，代表全长DJ-1，human起源
  • 推荐用于 mouse, rat 和 human 来源的DJ-1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 DJ-1 (D-4): sc-55572，可查看有标记的 DJ-1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是DJ-1 Antibody (A-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与DJ-1 Antibody (A-9) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。
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描述
基因信息
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说明书与实验方案
研究信息

関連項目

DJ-1 抗体（A-9）是一种小鼠单克隆 IgG 抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人样本中的 DJ-1。DJ-1 是一种高度保守的蛋白质，在细胞过程中发挥着至关重要的作用，尤其是在保护细胞免受氧化应激和维持线粒体功能方面。DJ-1 能通过与 PIASxα 形成复合物来调节雄激素受体，这突显了 DJ-1 在激素信号通路中的重要性，对癌症生物学和神经退行性疾病都有重要影响。编码 DJ-1 的基因位于染色体 1p36.23，该区域与肿瘤细胞的各种染色体异常有关，而该基因的突变与帕金森病有关，这突显了 DJ-1 与神经保护的相关性。DJ-1 广泛表达，并在细胞周期的 S 期从细胞质转位到细胞核，这表明 DJ-1 在细胞对生长刺激的反应中发挥着动态作用。抗 DJ-1 抗体（A-9）具有多方面的功能和对疾病的影响，是研究人员调查各种病症的分子机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DJ-1 抗体 (A-9) 参考文献:

  1. DJ-1作为人类癌基因和潜在的治疗靶点。  |  Cao, J., et al. 2015. Biochem Pharmacol. 93: 241-50. PMID: 25498803
  2. 癌细胞中DJ-1的表达：其与临床意义的相关性。  |  Kawate, T., et al. 2017. Adv Exp Med Biol. 1037: 45-59. PMID: 29147902
  3. DJ-1在帕金森病发病机理中的作用。  |  Dolgacheva, LP., et al. 2019. J Bioenerg Biomembr. 51: 175-188. PMID: 31054074
  4. DJ-1缺失可防止T细胞区免疫衰老。  |  Zeng, N., et al. 2022. EMBO Rep. 23: e53302. PMID: 35037711
  5. DJ-1对信号传导途径的调节：最新综述。  |  Neves, M., et al. 2022. Redox Biol. 51: 102283. PMID: 35303520
  6. DJ-1 可调控气道祖细胞/嗜酸性粒细胞的相互作用，从而促进过敏性炎症。  |  Li, K., et al. 2022. J Allergy Clin Immunol. 150: 1178-1193.e13. PMID: 35724763
  7. DJ-1通过下调星形胶质细胞中CHAC1的表达来抑制谷胱甘肽降解。  |  Ge, Y., et al. 2022. Neurosci Res. 184: 62-69. PMID: 35988816
  8. DJ-1分子伴侣活性通过与单体相互作用抑制Tau聚集倾向。  |  Jimenez-Harrison, D., et al. 2023. Biochemistry. 62: 976-988. PMID: 36813261
  9. DJ‑1对肾小球足细胞凋亡和线粒体自噬的影响。  |  Xiao, J., et al. 2023. Exp Ther Med. 26: 463. PMID: 37664676
  10. DJ-1可保护细胞免受GBA1缺乏引起的线粒体氧化应激。  |  Nam, Y., et al. 2024. Genes Genomics. 46: 519-529. PMID: 38460098

订购信息

产品名称产品编号规格价格数量收藏夹

DJ-1 抗体 (A-9)

sc-55573
200 µg/ml
$322.00

DJ-1 (A-9): m-IgGκ BP-HRP 套装

sc-521022
200 µg Ab, 40 µg BP
$361.00

I am using DJ-1 (A-9): sc-55573 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Elionore from Perfect antibodyClear results in Western Blots with this antibody. ICC results showed good signals as well.
Date published: 2019-11-27
Rated 4 out of 5 by Anonymous from antibodyAntibody was used in tissue and cells by western blotting. It worked.
Date published: 2019-07-26
Rated 5 out of 5 by cousins from This antibody is quite strongWe found a staining in the nucleus as well as in the cytoplasm. This antibody is quite strong. It works well.
Date published: 2017-02-15
Rated 5 out of 5 by FL from Clean blot with single strong band at expectedClean blot with single strong band at expected size using non-transfected 293T, mouse DJ-1 transfected 293T and SK-N-SH whole cell lysates. -SCBT QC
Date published: 2015-06-24
Rated 5 out of 5 by ET from Good specificity and signal strength in BTGood specificity and signal strength in BT-20 and HeLa whole cell lysates.. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by LR from WB publishable data with rat muscleWB publishable data with rat muscle - SCBT Publication Review
Date published: 2015-01-07
Rated 5 out of 5 by SM from Published data for WB in rat musclePublished data for WB in rat muscle. -SCBT Publication Review
Date published: 2015-01-05
Rated 5 out of 5 by MS from Beautiful immunoperoxidase nuclear and cytoplasmicBeautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2014-07-07
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DJ-1 抗体 (A-9) is rated 4.9 out of 5 by 8.
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