Date published: 2025-9-8
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DISC-1 Antibody (B-2) - Western Blotting - Image 355566
DIsc-1 抗体 (B-2): sc-365591. 在 SK-N-SH (A), MOLT-4 (B), A549 (C), RAW 264. 7 (D) 和 HeLa (E) 全细胞裂解液中 DIsc-1 表达的 Western 印迹分析.
DIsc-1 抗体 (B-2): sc-365591. 在 SK-N-SH (A), MOLT-4 (B), A549 (C), RAW 264. 7 (D) 和 HeLa (E) 全细胞裂解液中 DIsc-1 表达的 Western 印迹分析.

DISC-1 抗体 (B-2): sc-365591

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说明书
  • DISC-1 抗体 B-2 是小鼠单克隆 IgG2a κ，DISC-1抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列601-810位于human物种的DISC-1的C-terminus附近
  • DISC-1 抗体 (B-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 DISC-1
  • 抗DISC-1抗体（B-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DISC-1 (B-2): sc-365591的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是DISC-1 Antibody (B-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DISC-1 Antibody (B-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

DISC抗体

DISC-1抗体（B-2）是一种IgG2a κ小鼠单克隆DISC-1抗体（也称为DISC1抗体、精神分裂症断裂蛋白1抗体、C1orf136抗体或SCZD9抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的DISC-1蛋白。DISC-1抗体（B-2）既有非偶联形式的抗DISC-1抗体，也有多种偶联形式的抗DISC-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。"精神分裂症断裂基因"位点DISC与因染色体易位而患有精神分裂症的患者有关。DISC-1编码一种大蛋白，该蛋白预测包含一个球状N端结构域和一个螺旋C端结构域，这两个结构域都有可能与其他蛋白形成相互作用。DISC-1与参与中心体和细胞骨架系统的蛋白质相互作用，包括MIPT3、MAP1A和nudel；将受体定位到膜上的蛋白质，包括α-辅肌动蛋白-2和血影蛋白β IV；以及从膜受体传导信号的蛋白质，包括ATF-4和ATF-5。因此，DISC-1被认为参与细胞内运输、神经突结构和/或神经元迁移，所有这些都被认为是精神分裂症大脑的致病因素。DISC-1定位于细胞核，而突变型DISC-1的定位主要发生在细胞质中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DISC-1 抗体 (B-2) 参考文献:

  1. 精神分裂症间断期-1（DIsc-1）：突变截断阻止了与 NudE-like（NUDEL）的结合，抑制了神经元的生长。  |  Ozeki, Y., et al. 2003. Proc Natl Acad Sci U S A. 100: 289-94. PMID: 12506198
  2. DISC1（Disrupted-In-Schizophrenia 1）是一种与中心体相关的蛋白，它与 MAP1A, MIPT3, ATF4/5 和 NUDEL 相互作用：突变时的调控和相互作用的丧失。  |  Morris, JA., et al. 2003. Hum Mol Genet. 12: 1591-608. PMID: 12812986
  3. 精神分裂症候选基因 '精神分裂症中断 1 号 '参与神经元的生长。  |  Miyoshi, K., et al. 2003. Mol Psychiatry. 8: 685-94. PMID: 12874605
  4. 精神分裂症中断 1 和 Nudel 形成神经发育调控蛋白复合物：对精神分裂症和其他主要神经系统疾病的影响。  |  Brandon, NJ., et al. 2004. Mol Cell Neurosci. 25: 42-55. PMID: 14962739
  5. 精神分裂症断裂蛋白 1（DISC1）在成年和发育中小鼠大脑中的表达表明它在神经发育中的作用。  |  Schurov, IL., et al. 2004. Mol Psychiatry. 9: 1100-10. PMID: 15381924
  6. 精神分裂症间断期 1 型抑制 NUDEL（核分布元件样）寡肽酶的活性。  |  Hayashi, MA., et al. 2005. Proc Natl Acad Sci U S A. 102: 3828-33. PMID: 15728732
  7. DISC1 的亚细胞靶向依赖于一个独立于 Nudel 结合位点的结构域。  |  Brandon, NJ., et al. 2005. Mol Cell Neurosci. 28: 613-24. PMID: 15797709
  8. 精神分裂症干扰-1（DISC1）相互作用组与精神障碍：小鼠模型的影响。  |  Lipina, TV. and Roder, JC. 2014. Neurosci Biobehav Rev. 45: 271-94. PMID: 25016072
  9. DISC1 在神经元贩运中的作用及其对神经精神表现和神经治疗的影响  |  Tomoda, T., et al. 2017. Neurotherapeutics. 14: 623-629. PMID: 28664299
  10. DISC1 基因多态性与精神分裂症有关：来自荟萃分析的证据。  |  Wang, HY., et al. 2018. Prog Neuropsychopharmacol Biol Psychiatry. 81: 64-73. PMID: 29031911
  11. DISC1 突变小鼠的多糖基化  |  Takahashi, Y., et al. 2022. Int J Mol Sci. 23: PMID: 35563598
  12. 星形胶质细胞介导的 BLAWFS1 神经元调控缓解了 DISC1-N 小鼠的风险评估缺陷。  |  Zhou, X., et al. 2024. Neuron. 112: 2197-2217.e7. PMID: 38642554

订购信息

产品名称产品编号规格价格数量收藏夹

DISC-1 抗体 (B-2)

sc-365591
200 µg/ml
RMB2377.00

DISC-1 (B-2): m-IgG Fc BP-HRP 套装

sc-529399
200 µg Ab; 10 µg BP
RMB2662.00

DISC-1 (B-2): m-IgGκ BP-HRP 套装

sc-522279
200 µg Ab, 40 µg BP
RMB2662.00

DISC-1 (B-2): m-IgG2a BP-HRP 套装

sc-547309
200 µg Ab; 10 µg BP
RMB2662.00

DISC-1 抗体 (B-2) AC

sc-365591 AC
500 µg/ml, 25% agarose
RMB3129.00

DISC-1 抗体 (B-2) HRP

sc-365591 HRP
200 µg/ml
RMB2377.00

DISC-1 抗体 (B-2) FITC

sc-365591 FITC
200 µg/ml
RMB2482.00

DISC-1 抗体 (B-2) PE

sc-365591 PE
200 µg/ml
RMB2580.00

DISC-1 抗体 (B-2) Alexa Fluor® 488

sc-365591 AF488
200 µg/ml
RMB2685.00

DISC-1 抗体 (B-2) Alexa Fluor® 546

sc-365591 AF546
200 µg/ml
RMB2685.00

DISC-1 抗体 (B-2) Alexa Fluor® 594

sc-365591 AF594
200 µg/ml
RMB2685.00

DISC-1 抗体 (B-2) Alexa Fluor® 647

sc-365591 AF647
200 µg/ml
RMB2685.00

DISC-1 抗体 (B-2) Alexa Fluor® 680

sc-365591 AF680
200 µg/ml
RMB2685.00

DISC-1 抗体 (B-2) Alexa Fluor® 790

sc-365591 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with DISC-1 (B-2): sc-365591 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WBWe used Disc-1 (B-2) antibody for WB in two different cell lines, Human SCC25 cells and Mouse lung primary fibroblast, and it worked well. We used anti-mouse IgG.
Date published: 2018-03-28
Rated 5 out of 5 by from Great features!I got the sample a month ago and I am so happy it did well in my myeloma cell line(8226).Thank you for your gift!
Date published: 2018-01-08
Rated 5 out of 5 by from Works wellGot a specific band of the expected molecular weight in western blot of HEK293T cell lysates when used at 1:1000 in 3% BSA/T-PBS.
Date published: 2017-09-18
Rated 5 out of 5 by from Strong signalClean band at expected molecular weight in rat hippocampus tissue
Date published: 2017-08-15
Rated 5 out of 5 by from Good western blot detection in mouse samplesI tried this antibody for western blot on samples from primary neurons as well as whole brain tissue from mice and detected strong signal. It may be picking up alternative splice isoforms as well because I see multiple bands above the strongest signal. Used at 1:1000 and signal was great.
Date published: 2017-02-02
Rated 5 out of 5 by from Perfect western blot expression detectionWe used this antibody in a western blot experiment for expression detection and got a perfect band at the correct molecular weight in HeLa cells
Date published: 2017-01-30
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-09
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DISC-1 抗体 (B-2) is rated 5.0 out of 5 by 8.
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