Date published: 2025-9-9
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DEPTOR Antibody (A-3) - Western Blotting - Image 301487
DEPTOR 抗体 (A-3): sc-398169. 未转染 293T: sc-117752 (A), 人 DEPTOR 转染 293T: sc-128441 (B), Ramos (C), U266 (D), MCF7 (E) 和 COLO 320DM (F) 全细胞裂解液中 DEPTOR 表达的 Western 印迹分析.
DEPTOR Antibody (A-3) - Western Blotting - Image 301487
DEPTOR 抗体 (A-3): sc-398169. 未转染 293T: sc-117752 (A), 人 DEPTOR 转染 293T: sc-128441 (B), Ramos (C), U266 (D), MCF7 (E) 和 COLO 320DM (F) 全细胞裂解液中 DEPTOR 表达的 Western 印迹分析.
DEPTOR Antibody (A-3) - Western Blotting - Image 363880
DEPTOR 抗体 (A-3): sc-398169. 3T3-L1 (A) 和 NIH/3T3 (B) 全细胞裂解液中 DEPTOR 表达的 Western 印迹分析.
DEPTOR Antibody (A-3) - Western Blotting - Image 387414
DEPTOR 抗体 (A-3) HRP: sc-398169 HRP. 未转染 293T: sc-117752 (A), 转染人 DEPTOR 的 293T: sc-128441 (B), Ramos (C) 和 NCI-H1299 (D) 全细胞裂解液中 DEPTOR 表达的直接 Western 印迹分析.
DEPTOR 抗体 (A-3): sc-398169. 未转染 293T: sc-117752 (A), 人 DEPTOR 转染 293T: sc-128441 (B), Ramos (C), U266 (D), MCF7 (E) 和 COLO 320DM (F) 全细胞裂解液中 DEPTOR 表达的 Western 印迹分析.

DEPTOR 抗体 (A-3): sc-398169

4.1(8)
寫評論提問

说明书
  • DEPTOR 抗体 A-3 是小鼠单克隆 IgG1 κ，DEPTOR抗体, 在11篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的DEPTOR的N-terminus附近的氨基酸18-37之间
  • DEPTOR 抗体 (A-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 DEPTOR
  • 抗DEPTOR抗体（A-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DEPTOR (A-3): sc-398169的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是DEPTOR Antibody (A-3) for WB applications. 的首选辅助检测试剂，这些试剂现与DEPTOR Antibody (A-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

DEPTOR抗体（A-3）是一种IgG1 κ小鼠单克隆DEPTOR抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的DEPTOR蛋白。DEPTOR抗体（A-3）既可以是非偶联的抗DEPTOR抗体形式，也可以是多种偶联形式的抗DEPTOR抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。DEPTOR（含有DEP结构域并与MTOR相互作用的蛋白），也称为DEP.6或DEPDC6（含有DEP结构域的蛋白6），是一种由409个氨基酸组成的蛋白质，对mTORC1和mTORC2通路起负调控作用。DEPTOR通过其PDZ结构域与FRAP相互作用，并经历翻译后磷酸化。DEPTOR含有两个DEP结构域和一个PDZ（DHR）结构域，其编码基因定位于人类染色体8q24.12。染色体8由近1.46亿个碱基对组成，编码800多个基因，并与多种疾病和恶性肿瘤相关。精神分裂症、双相情感障碍、8号染色体三体、Pfeiffer综合征、先天性甲状腺功能减退症、Waardenburg综合征以及某些白血病和淋巴瘤被认为是由于定位于染色体8的特定基因缺陷所导致的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DEPTOR 抗体 (A-3) 参考文献:

  1. 在引入了正常 8 号染色体的维尔纳综合征细胞系中优先表达完整的 WRN 基因。  |  Kashino, G., et al. 2001. Biochem Biophys Res Commun. 289: 111-5. PMID: 11708785
  2. II 型瓦登堡综合征新基因座的细胞遗传学图谱。  |  Selicorni, A., et al. 2002. Hum Genet. 110: 64-7. PMID: 11810298
  3. 通过对 11 项躁狂症关联研究的综合分析，有力地证明了 6q 和 8q 染色体上的易感基因位点。  |  McQueen, MB., et al. 2005. Am J Hum Genet. 77: 582-95. PMID: 16175504
  4. 人类癌症中早衰 Werner 综合征基因的表观遗传失活。  |  Agrelo, R., et al. 2006. Proc Natl Acad Sci U S A. 103: 8822-7. PMID: 16723399
  5. 具有 Burkitt 样细胞的非霍奇金淋巴瘤与 c-Myc 扩增和预后不良缔合作用。  |  Mossafa, H., et al. 2006. Leuk Lymphoma. 47: 1885-93. PMID: 17065002
  6. 肾细胞癌中的 mTOR：肿瘤生物学调节器和治疗靶点。  |  Wysocki, PJ. 2009. Expert Rev Mol Diagn. 9: 231-41. PMID: 19379082
  7. DEPTOR 是一种 mTOR 阻抑剂，经常在多发性骨髓瘤细胞中过表达，是细胞存活所必需的。  |  Peterson, TR., et al. 2009. Cell. 137: 873-86. PMID: 19446321
  8. DEPTOR 在癌症、新陈代谢和免疫力之间的联系。  |  Caron, A., et al. 2018. Physiol Rev. 98: 1765-1803. PMID: 29897294
  9. DEPTOR 稳定 ErbB2，促进 ErbB2 阳性乳腺癌细胞的增殖和存活。  |  Bi, Y., et al. 2021. Theranostics. 11: 6355-6369. PMID: 33995662
  10. 骨骼发育和疾病中的 DEPTOR。  |  Perez-Tejeiro, JM. and Csukasi, F. 2021. Front Genet. 12: 667283. PMID: 34122519
  11. UBE2C/CDH1/DEPTOR 轴是肺癌细胞中的致癌基因和抑癌基因级联。  |  Zhang, S., et al. 2023. J Clin Invest. 133: PMID: 36548081
  12. PUM1通过激活DEPTOR诱导的胃癌糖酵解促进肿瘤进展  |  Yin, S., et al. 2023. Adv Sci (Weinh). 10: e2301190. PMID: 37469018

订购信息

产品名称产品编号规格价格数量收藏夹

DEPTOR 抗体 (A-3)

sc-398169
200 µg/ml
RMB2377.00

DEPTOR (A-3): m-IgG Fc BP-HRP 套装

sc-527745
200 µg Ab; 10 µg BP
RMB2662.00

DEPTOR (A-3): m-IgG1 BP-HRP 套装

sc-533118
200 µg Ab; 20 µg BP
RMB2662.00

DEPTOR 抗体 (A-3) AC

sc-398169 AC
500 µg/ml, 25% agarose
RMB3129.00

DEPTOR 抗体 (A-3) HRP

sc-398169 HRP
200 µg/ml
RMB2377.00

DEPTOR 抗体 (A-3) FITC

sc-398169 FITC
200 µg/ml
RMB2482.00

DEPTOR 抗体 (A-3) PE

sc-398169 PE
200 µg/ml
RMB2580.00

DEPTOR 抗体 (A-3) Alexa Fluor® 488

sc-398169 AF488
200 µg/ml
RMB2685.00

DEPTOR 抗体 (A-3) Alexa Fluor® 546

sc-398169 AF546
200 µg/ml
RMB2685.00

DEPTOR 抗体 (A-3) Alexa Fluor® 594

sc-398169 AF594
200 µg/ml
RMB2685.00

DEPTOR 抗体 (A-3) Alexa Fluor® 647

sc-398169 AF647
200 µg/ml
RMB2685.00

DEPTOR 抗体 (A-3) Alexa Fluor® 680

sc-398169 AF680
200 µg/ml
RMB2685.00

DEPTOR 抗体 (A-3) Alexa Fluor® 790

sc-398169 AF790
200 µg/ml
RMB2685.00

DEPTOR (A-3) 中和勝肽

sc-398169 P
100 µg/0.5 ml
RMB511.00

I am wondering whether you have data of DEPTOR Antibody (A-3) PE for flow.

Asked by: Peter
Thank you for your question. We can not supply data but we do have a 1 year warranty from date of purchase .
Answered by: BlakeJ
Date published: 2023-12-18

I run a project on DEPTOR for the Boston children's Hospital and I would receive the free sample to test your antibody, is it possible?

Asked by: sylvain
Thank you for your question. For a limited time we are offering a free 10 µg sample of our mouse monoclonal antibody, DEPTOR (A-3): sc-398169. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-09-19

What application is the blocking peptide sc-398169 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from good2 dark clear bands, one at 48kDa as predicted and the other one on 60kDa
Date published: 2020-02-06
Rated 5 out of 5 by from good for WBIt worked for WB of mouse protein in tissue extract at 1:1000 (3% BSA in PBST).
Date published: 2018-02-15
Rated 5 out of 5 by from specific antibodyI got the sample 2 months ago, the antibody can detect a very strong band in deptor overexpressed 293T cell, but not non-transfected 293T cells.
Date published: 2017-12-11
Rated 5 out of 5 by from DEPTORThis antibody worked well for DEPTOR detection in ES mouse cells. Sharp bands with recommended use!
Date published: 2017-10-03
Rated 1 out of 5 by from Didn't worrk in Si samples western blottingAntibody was diluted 1000X and incubated at 4℃ overnight. Si Deptor mouse 3T3L1 cell samples was aim to verify which band was specific. The Previous one is antibody from other company. The new one is from Santa. Santa Deptor antibody has several bands in which no band corresponded to si Deptor. This antibody didn’t work.
Date published: 2017-08-18
Rated 5 out of 5 by from Strong and reliable for Western blotNice and solid Western blot data. Reliable and specific, it's a wise choice!
Date published: 2017-01-25
Rated 4 out of 5 by from good for wbIt works good for western blot detection in HepG2 cells. the dilution is 1:500.
Date published: 2016-12-09
Rated 5 out of 5 by from Good Western blot data of DEPTOR expression in nonGood Western blot data of DEPTOR expression in non-transfected 293T, human DEPTOR transfected 293T Ramos, U266, MCF7 and COLO 320DM whole cell lysates. -SCBT QC
Date published: 2014-04-30
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DEPTOR 抗体 (A-3) is rated 4.1 out of 5 by 8.
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