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DEC1 抗体 (S-8): sc-101023

4.5(8)
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说明书
  • DEC1 抗体 S-8 是小鼠单克隆 IgG2b κ,DEC1抗体, 在16篇文献中引用,规格为100 µg/ml
  • 针对与human的DEC1的内部区域对应的重组蛋白的部分区域
  • 推荐用于 mouse, rat 和 human 来源的DEC1 WB, IP 和 ELISA检测
  • 关于如何获取DEC1 (S-8): sc-101023免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是DEC1 Antibody (S-8) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与DEC1 Antibody (S-8) 打包提供(请参阅下面的订购信息)。

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関連項目

DEC1抗体(S-8)是一种IgG2b κ小鼠单克隆DEC1抗体(也称为DEC1抗体),可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的DEC1蛋白。DEC1抗体(S-8)为非偶联形式的抗DEC1抗体。人类DEC1,也称为SHARP2,是一种含有412个氨基酸的碱性螺旋-环-螺旋(bHLH)蛋白,参与控制多种细胞类型(包括神经细胞、成纤维细胞和软骨细胞)的增殖和/或分化。DEC1的bHLH区域在结构上与哺乳动物HES家族、果蝇hairy和果蝇Enhancer of split m7的bHLH区域相似。DEC1是cAMP在多种细胞中的新型直接靶点,参与控制cAMP激活细胞中的基因表达。短暂的光脉冲以相位依赖的方式诱导DEC1的表达。DEC1在软骨、肠道、肺和脾脏中高表达。DEC1和DEC2通过抑制特定时钟基因的转录在调节哺乳动物分子时钟方面发挥作用。DEC1和DEC2均以昼夜节律的方式在视交叉上核中检测到。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DEC1 抗体 (S-8) 参考文献:

  1. 基本螺旋-环-螺旋蛋白新成员 DEC2 的分子克隆和特征描述。  |  Fujimoto, K., et al. 2001. Biochem Biophys Res Commun. 280: 164-71. PMID: 11162494
  2. 基本螺旋环螺旋蛋白 DEC1 (BHLHB2)/Stra13/Sharp2 对环磷酸腺苷通路的诱导。  |  Shen, M., et al. 2001. Eur J Cell Biol. 80: 329-34. PMID: 11432722
  3. DEC1 是 TGF-beta 的下游靶标,具有序列特异性转录抑制活性。  |  Zawel, L., et al. 2002. Proc Natl Acad Sci U S A. 99: 2848-53. PMID: 11880636
  4. Dec1 和 Dec2 是哺乳动物分子钟的调节器。  |  Honma, S., et al. 2002. Nature. 419: 841-4. PMID: 12397359
  5. 分化的人类胚胎软骨细胞中表达的新型碱性螺旋环螺旋蛋白 DEC1 的分子特征。  |  Shen, M., et al. 1997. Biochem Biophys Res Commun. 236: 294-8. PMID: 9240428

订购信息

产品名称产品编号规格价格数量收藏夹

DEC1 抗体 (S-8)

sc-101023
100 µg/ml
RMB2504.00

DEC1 (S-8): m-IgG Fc BP-HRP 套装

sc-539730
100 µg Ab; 10 µg BP
RMB2662.00

DEC1 (S-8): m-IgG2b BP-HRP 套装

sc-549746
100 µg Ab; 10 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for DEC1 (S-8): sc-101023?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-101023.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 13
Date published: 2017-01-26
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Rated 5 out of 5 by from goodthis antibody works well in skeletal muscle of mice.
Date published: 2022-10-01
Rated 5 out of 5 by from Very goodVery good and clean bands through western blot analysis
Date published: 2018-10-12
Rated 3 out of 5 by from Chip assayに有用1ヶ月前に購入しましたが、Western blotやChip assayに有用と思われます。免疫染色はいまいちのようです。
Date published: 2018-09-03
Rated 4 out of 5 by from Good antibodyWorks very good. The antibody sample works fine, gives a sharp band at 43kDa region.
Date published: 2018-06-19
Rated 5 out of 5 by from Worked well in western blotUsed the antibody at 1:1000 with about 15ug of protein loaded and could detect differences in expression effectively.
Date published: 2018-05-27
Rated 5 out of 5 by from Excelente resultado en WBHe utilizado este anticuerpo para WB, detectando una sola banda en Fibroblastos humanos y células de osteosarcoma
Date published: 2017-12-11
Rated 5 out of 5 by from Good WB antibodyDesired results produced with DEC1 Antibody (S-8) by WB. Specific band detected
Date published: 2017-01-21
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DEC1 抗体 (S-8) is rated 4.5 out of 5 by 8.
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