Date published: 2026-10-24

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DARPP-32 Antibody (G-5) - Western Blotting - Image 318535
DARPP-32 抗体 (G-5): sc-398360. 大鼠小脑 (A) 和人小脑 (B) 组织提取物及 SH-SY5Y 全细胞裂解液 (C) 中 DARPP-32 表达的 Western 印迹分析.
DARPP-32 抗体 (G-5): sc-398360. 大鼠小脑 (A) 和人小脑 (B) 组织提取物及 SH-SY5Y 全细胞裂解液 (C) 中 DARPP-32 表达的 Western 印迹分析.

DARPP-32 抗体 (G-5): sc-398360

5.0(2)
寫評論提問
说明书
  • DARPP-32抗体(G-5)是小鼠单克隆IgG2a κ, 在2篇文献中引用，规格为200 µg/ml
  • 针对氨基酸134-195的图谱，与DARPP-32的134-195相对应的表位，bovine来源
  • 推荐用于 mouse, rat 和 human 来源的DARPP-32 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： bovine
  • 看 DARPP-32 (H-3): sc-271111，可查看有标记的 DARPP-32 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是DARPP-32 Antibody (G-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与DARPP-32 Antibody (G-5) 打包提供（请参阅下面的订购信息）。
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関連項目

附加DARPP抗体，包括DARPP-32

多巴胺能信号通路对多种脑功能至关重要，在精神分裂症、帕金森病和药物滥用等几种神经系统疾病中多巴胺能信号通路均存在异常。DARPP-32（多巴胺和腺苷3',5'-单磷酸调节的磷酸蛋白）在接受多巴胺能输入的神经元中含量丰富。PKA的激活和DARPP-32在苏氨酸上的磷酸化发生在多巴胺作用于D1样受体后的反应中。多巴胺与D2样受体的相互作用导致PKA活化的抑制、蛋白磷酸酶2B的活化以及DARPP-32的随后去磷酸化。多巴胺以外的神经递质也可能刺激DARPP-32的磷酸化或去磷酸化。磷酸化的DARPP-32是PP-1的有效抑制剂。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DARPP-32 抗体 (G-5) 参考文献:

  1. DARPP-32介导前脑中的5-羟色胺神经传递。  |  Svenningsson, P., et al. 2002. Proc Natl Acad Sci U S A. 99: 3188-93. PMID: 11880652
  2. 通过多巴胺和cAMP调节的磷蛋白DARPP-32控制肾脏中的电解质转运。  |  Fryckstedt, J., et al. 1992. J Auton Pharmacol. 12: 183-9. PMID: 1321155
  3. DARPP-32表达在食管鳞状细胞癌的发育不良期后出现。  |  Ebihara, Y., et al. 2004. Br J Cancer. 91: 119-23. PMID: 15188007
  4. DARPP-32 磷酸化会抑制尼古丁的行为效应。  |  Zhu, H., et al. 2005. Biol Psychiatry. 58: 981-9. PMID: 16084497
  5. DARPP-32是多巴胺和谷氨酸信号的强效整合器。  |  Fernandez, E., et al. 2006. PLoS Comput Biol. 2: e176. PMID: 17194217
  6. DARPP-32 增加了表皮生长因子受体和 ERBB3 之间的相互作用，从而促进肿瘤对吉非替尼的耐药性。  |  Zhu, S., et al. 2011. Gastroenterology. 141: 1738-48.e1-2. PMID: 21741919
  7. 精神分裂症患者死后大脑中DARPP-32的详细表达谱：一项免疫组织化学研究。  |  Kunii, Y., et al. 2011. Med Mol Morphol. 44: 190-9. PMID: 22179181
  8. DARPP-32基因变异对前额叶皮层体积和事件记忆表现的影响。  |  Persson, N., et al. 2017. Front Neurosci. 11: 244. PMID: 28553197
  9. 幽门螺杆菌对miR490-3p的沉默会激活DARPP-32并诱导对吉非替尼的耐药性。  |  Zhu, S., et al. 2020. Cancer Lett. 491: 87-96. PMID: 32735911
  10. 达格列净通过激活PKM2/PP1/FUNDC1依赖性线粒体自噬来保护心脏功能免受4型心肾综合征的影响。  |  Saidy, B., et al. 2021. J Cell Mol Med. 25: 5015-5024. PMID: 33991172

订购信息

产品名称产品编号规格价格数量收藏夹

DARPP-32 抗体 (G-5)

sc-398360
200 µg/ml
CNY2422.00

DARPP-32 (G-5): m-IgG Fc BP-HRP 套装

sc-538425
200 µg Ab; 10 µg BP
CNY2715.00

DARPP-32 (G-5): m-IgGκ BP-HRP 套装

sc-536038
200 µg Ab; 40 µg BP
CNY2715.00

Can Darpp-32 (G-5) Ab be used to detect total Darpp-32 from? ( Truncated, Full-length Darpp-32) Additionally, What's the difference between G-5 and F-3 Darpp-32 Ab?

Asked by: bami
Thank you for your question. I have checked this product. The immunogen information of this product is 'raised against amino acids 134-195 mapping epitope corresponding to amino acids 134-195 of DARPP-32 of bovine origin'. Also we do not have the Darpp-32 (F-3) antibody, so I have no idea about the difference between G-5 and F-3. I don't know the F-3 is a monoclonal antibody or a polyclonal antibody and their host species. Usually, in parentheses, such as (G-5) and (F-3) are the clone numbers, which are randomly coded.
Answered by: Ling Chen
Date published: 2022-01-11

Can DARPP-32 (G-5): sc-398360 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, DARPP-32 (G-5): sc-398360 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by ET from Satisfactory Western Blot data of DARPPSatisfactory Western Blot data of DARPP-32 expression in mouse brain and human hippocampus tissue extracts. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by TO from Good Western Blot data of DARPPGood Western Blot data of DARPP-32 expression in rat cerebellum and human cerebellum tissue extracts and SH-SY5Y whole cell lysate . -SCBT QC
Date published: 2014-05-29
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DARPP-32 抗体 (G-5) is rated 5.0 out of 5 by 2.
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