Date published: 2025-9-8
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D2DR/Dopamine D2 Receptor Antibody (B-10) - Western Blotting - Image 391012
D2DR 抗体 (B-10) Alexa Fluor® 680: sc-5303 AF680. 直接对 AtT-20/D16vF2 全细胞裂解液中 D2DR 的表达进行近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
D2DR 抗体 (B-10) Alexa Fluor® 680: sc-5303 AF680. 直接对 AtT-20/D16vF2 全细胞裂解液中 D2DR 的表达进行近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.

D2DR/Dopamine D2 Receptor 抗体 (B-10): sc-5303

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说明书
  • D2DR/Dopamine D2 Receptor 抗体 B-10 是小鼠单克隆 IgG2a κ，D2DR/Dopamine D2 Receptor抗体, 在169篇文献中引用，规格为200 µg/ml
  • 免疫human物种的dopamine receptor type 2的氨基酸1-50
  • D2DR/Dopamine D2 Receptor 抗体 (B-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 D2DR long and short forms
  • 抗D2DR/Dopamine D2 Receptor抗体（B-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取D2DR/Dopamine D2 Receptor (B-10): sc-5303的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 D2DR/Dopamine D2 Receptor 抗体 (B-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与D2DR/Dopamine D2 Receptor 抗体 (B-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

D2DR抗体（B-10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的D2DR。抗D2DR抗体（B-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。D2DR是G蛋白偶联受体家族的一员，通过调节多巴胺的作用在中枢神经系统中发挥关键作用。多巴胺是一种重要的神经递质，参与调节情绪、奖励和运动控制。D2DR主要位于大脑中，特别是纹状体和垂体等区域，在这些区域中，D2DR对于调节神经传递和影响各种生理过程至关重要。D2DR信号传导与多种神经精神疾病有关，包括精神分裂症和帕金森病，因为其正常功能至关重要。了解D2DR的定位和功能对于开发能够有效解决这些问题的靶向疗法非常重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

D2DR/Dopamine D2 Receptor 抗体 (B-10) 参考文献:

  1. 多巴胺 D2 受体的结构亚型在功能上截然不同：克隆的 D2A 和 D2B 亚型在异源细胞系中的表达。  |  Hayes, G., et al. 1992. Mol Endocrinol. 6: 920-6. PMID: 1323056
  2. 两种激酶介导了依赖于激动剂的 beta 2-肾上腺素能受体的磷酸化和脱敏。  |  Hausdorff, WP., et al. 1990. Symp Soc Exp Biol. 44: 225-40. PMID: 1983369
  3. 真核细胞中表达的α-肾上腺素能受体亚型的多种第二信使途径。  |  Cotecchia, S., et al. 1990. J Biol Chem. 265: 63-9. PMID: 2152928
  4. 以嗜铬细胞瘤中的多巴胺受体亚型2（D2DR）为靶点：体外与体内研究结果的正面比较  |  Saveanu, A., et al. 2013. J Clin Endocrinol Metab. 98: E1951-5. PMID: 24009136
  5. 左旋延胡索碱选择性阻断脊髓D2DR，通过抑制PI3K/Akt-MAPK信号传导，以依赖吗啡受体（MOR）的方式减轻吗啡耐受性。  |  Dai, WL., et al. 2018. Exp Mol Med. 50: 1-12. PMID: 30429454
  6. 大鼠脊髓中的多巴胺D1-D2DR复合物在慢性压迫损伤后通过增加神经元的兴奋性来促进神经性疼痛。  |  Bao, YN., et al. 2021. Exp Mol Med. 53: 235-249. PMID: 33558591
  7. 一种新型的多巴胺D2受体-NR2B蛋白复合物可能与吗啡使用障碍有关。  |  Li, A., et al. 2023. Eur J Pharmacol. 961: 176174. PMID: 37939993
  8. 激动剂诱导的多巴胺 D1 受体刺激腺苷酸环化酶活性脱敏与 D1 受体内化在时间和生物化学上是分离的。  |  Ng, GY., et al. 1995. Proc Natl Acad Sci U S A. 92: 10157-61. PMID: 7479745
  9. G 蛋白偶联受体超家族中保守的七跨膜序列 NP(X)2,3Y 调节着 beta 2-肾上腺素能受体的多种特性。  |  Barak, LS., et al. 1995. Biochemistry. 34: 15407-14. PMID: 7492540
  10. 多巴胺受体亚型的分子和化学神经药理学。  |  Ogawa, N. 1995. Acta Med Okayama. 49: 1-11. PMID: 7762403
  11. S311C D2DR变异：与精神分裂症无关。  |  Sobell, J., et al. 1994. Lancet. 344: 621-2. PMID: 7914994
  12. D2 多巴胺受体异构体通过不同的 Gi alpha 蛋白发出信号以抑制腺苷酸环化酶。利用定点突变的 Gi alpha 蛋白进行的研究。  |  Senogles, SE. 1994. J Biol Chem. 269: 23120-7. PMID: 7916015

订购信息

产品名称产品编号规格价格数量收藏夹

D2DR/Dopamine D2 Receptor 抗体 (B-10)

sc-5303
200 µg/ml
RMB2377.00

D2DR/Dopamine D2 Receptor (B-10): m-IgG Fc BP-HRP 套装

sc-528157
200 µg Ab; 10 µg BP
RMB2662.00

D2DR/Dopamine D2 Receptor (B-10): m-IgGκ BP-HRP 套装

sc-520465
200 µg Ab, 40 µg BP
RMB2662.00

D2DR/Dopamine D2 Receptor (B-10): m-IgG2a BP-HRP 套装

sc-547034
200 µg Ab; 10 µg BP
RMB2662.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) AC

sc-5303 AC
500 µg/ml, 25% agarose
RMB3129.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) HRP

sc-5303 HRP
200 µg/ml
RMB2377.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) FITC

sc-5303 FITC
200 µg/ml
RMB2482.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) PE

sc-5303 PE
200 µg/ml
RMB2580.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) Alexa Fluor® 488

sc-5303 AF488
200 µg/ml
RMB2685.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) Alexa Fluor® 546

sc-5303 AF546
200 µg/ml
RMB2685.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) Alexa Fluor® 594

sc-5303 AF594
200 µg/ml
RMB2685.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) Alexa Fluor® 647

sc-5303 AF647
200 µg/ml
RMB2685.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) Alexa Fluor® 680

sc-5303 AF680
200 µg/ml
RMB2685.00

D2DR/Dopamine D2 Receptor 抗体 (B-10) Alexa Fluor® 790

sc-5303 AF790
200 µg/ml
RMB2685.00

Is this antibody available again? We tried to acquire this Ab in Mexico 6 months ago and were told that the antibody and this same cell origin Ab for D1R were out of stock.

Asked by: Alfredo Y
Thank you for your question. For accurate availability, pricing and shipping information regarding this antibody product, please contact your local distributor for assistance.
Answered by: Technical Service
Date published: 2025-01-08

What is the suggested loading scheme amount in the Western Blot procedure? Thank you

Asked by: hanna_k77
Thank you for your question, We recommend (40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane). We also recommend a dilution of 1:100 with our antibodies.
Answered by: Tech Service 1
Date published: 2020-11-12

Could you conjugate biotin to D2R2 antibody(https://www.scbt.com/scbt/search?Ntt=sc-5303)? Thank you!

Asked by: Yingying2703
Thank you for your question. Please contact our Special Orders Department to inquire about special conjugations for this antibody. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Tech Service
Date published: 2019-06-12

Which monoclonal D2R antibodies are neutralizing, and are they available in bulk?

Asked by: Phlp
Thank you for your question. We have not tested this antibody for a neutralizing research application, and are not aware of any publications citing use of this antibody in that application. We are able to provide this antibody in bulk quantities. Please contact our Special Orders Department for a quotation. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2019-04-20

Is there a blocking peptide available for DRD2R B-10 for use in IHC?

Asked by: slpMountaineer
Thank you for your question. No, unfortunately we do not offer a blocking peptide for this antibody as it was not made against a peptide. You can use a normal mouse IgG as a negative control.
Answered by: Tech Service
Date published: 2019-01-14

Can you suggest which antibody is preferable for D2DR, S|D#DR and D1DR detection using Array Tomography? Rat

Asked by: Iya Zhvania
We offer the following antibodies against these epitopes: D2DR Antibody (B-10): sc-5303; recommended for detection of D2DR long and short forms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA https://www.scbt.com/scbt/product/d2dr-antibody-b-10 D1DR Antibody (SG2-D1a): sc-33660; recommended for detection of D1DR of mouse, rat and human origin by WB, IP and IF https://www.scbt.com/scbt/product/d1dr-antibody-sg2-d1a For further information, please contact our technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-21
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Rated 1 out of 5 by from Quality of antibody for Drd2 may be an issue.It’s not working. I have tried twice and didn’t not detect any band.
Date published: 2024-11-03
Rated 4 out of 5 by from goodWork well in WB at 1:1000, diluted in 5% skim milk, 4C over night, rat brain sample.
Date published: 2020-10-12
Rated 5 out of 5 by from Best for ImmunoFluorescenceWe used this antibody to detect D2 receptor by IF on mouse brain tissue and obtained a very clean signal.
Date published: 2020-08-17
Rated 4 out of 5 by from Very good IF resultsWe tested the antibody by IF on brain tissue and obtained a very nice specific signal.
Date published: 2018-03-20
Rated 4 out of 5 by from I can not detect the expression of DRD2 in HUVECI can not detect the expression of DRD2 in HUVEC , maybe in HUVEC the protein of DRD2 is too low
Date published: 2017-10-09
Rated 4 out of 5 by from Clear band below 48 kDa in western blotting.Clear band below 48 kDa in western blotting. But I wondered whether it is the right band. Because D2DR can be glycosylated. Should it be higher than 48 kDa?
Date published: 2017-09-29
Rated 5 out of 5 by from Great futuresI got the sample free and it worked perfectly with all my lab mates. At least 4 of us tried this AB to their respective sample and it works great. Thanks.
Date published: 2017-08-04
Rated 5 out of 5 by from repeatabilityWork well in WB at 1:300, PC12 cells ,after repeated use three times the effect is still very well.
Date published: 2017-07-04
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D2DR/Dopamine D2 Receptor 抗体 (B-10) is rated 4.3 out of 5 by 25.
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