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Cytokeratin 8 抗体 (M20): sc-52324

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说明书
  • Cytokeratin 8 抗体 M20 是小鼠单克隆 IgG1, 在11篇文献中引用,提供 100 µg/ml
  • 针对源自human的乳腺癌细胞系MCF7分离出的角蛋白
  • 推荐用于 mouse, rat 和 human 来源的Cytokeratin 8 WB, IP, IF 和 FCM检测
  • 看 Cytokeratin 8 (C51): sc-8020,可查看有标记的 Cytokeratin 8 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成Cytokeratin 8 抗体 (M20)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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细胞角蛋白是一组多样化的中间丝蛋白(IFPs),在角质化和非角质化上皮组织中成对表达。细胞角蛋白在维持上皮细胞整体结构完整性的分化、组织特化和功能方面发挥着关键作用。它们已被发现是有用的组织分化标记物,可直接应用于恶性肿瘤的表征。细胞角蛋白8在上皮和上皮源性肿瘤中表达。细胞角蛋白8和18对通常在单纯上皮中表达,但在分层上皮细胞中不表达。研究表明,分层上皮来源的鳞状细胞癌显示细胞角蛋白8和18的表达异常,尽管尚不清楚这些蛋白质是否有助于细胞的恶性表型。口腔鳞状细胞癌中细胞角蛋白8和18的表达是一种提示预后不良的独立预后标志物。细胞角蛋白8的表达与白斑和头颈部癌的恶性程度相关;它在所有非小细胞肺癌中都有表达。细胞角蛋白8已被证明在肿瘤细胞上具有细胞外表位,这可能是有价值的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 8 抗体 (M20) 参考文献:

  1. 人类鼻咽癌细胞中细胞角蛋白8的沉默导致顺铂敏感性增强。  |  Wang, Y., et al. 2008. Cancer Lett. 265: 188-96. PMID: 18353540
  2. 细胞角蛋白8外质域将尿激酶型纤溶酶原激活剂与乳腺癌细胞结合,并调节其粘附性、生长和侵袭性。  |  Obermajer, N., et al. 2009. Mol Cancer. 8: 88. PMID: 19845941
  3. 细胞角蛋白8与凝集因子B相互作用:一种新的可能的毒力因子靶标。  |  Haim, M., et al. 2010. Microbiology (Reading). 156: 3710-3721. PMID: 20817646
  4. 细胞角蛋白8在人类角膜结膜上皮中表达,特别是在角膜缘上皮细胞中。  |  Merjava, S., et al. 2011. Invest Ophthalmol Vis Sci. 52: 787-94. PMID: 20926822
  5. 细胞角蛋白-8与F508del-CFTR的相互作用中断可纠正其功能缺陷。  |  Colas, J., et al. 2012. Hum Mol Genet. 21: 623-34. PMID: 22038833
  6. 丙型肝炎病毒细胞中的细胞角蛋白8增加,其异位表达诱导SMMC7721细胞凋亡。  |  Sun, MZ., et al. 2013. World J Gastroenterol. 19: 6178-87. PMID: 24115814
  7. 细胞角蛋白8-MHC I类相互作用:淋巴结转移癌细胞系的一种潜在的新型免疫逃逸表型。  |  Wu, MS., et al. 2013. Biochem Biophys Res Commun. 441: 618-23. PMID: 24183726
  8. 人类牙齿发育过程中细胞角蛋白8、波形蛋白、syndecan-1和Ki-67的表达。  |  Kero, D., et al. 2014. J Mol Histol. 45: 627-40. PMID: 25120060
  9. 甲状腺无节细胞癌中的细胞角蛋白-8:不仅仅是一种简单的细胞骨架结构蛋白  |  Guo, D., et al. 2018. Int J Mol Sci. 19: PMID: 29443941
  10. 通过未折叠蛋白反应的PERK/eIF2α臂加重内质网应激和阻断自噬,敲除细胞角蛋白8可克服脊索瘤细胞的化疗耐药性。  |  Wang, D., et al. 2019. Cell Death Dis. 10: 887. PMID: 31767864

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 8 抗体 (M20)

sc-52324
100 µg/ml
$322.00

What is the molecular weight of Cytokeratin 8 ?

Asked by: jenniferc
Thank you for your question. Cytokeratin 8 has an expected molecular weight of approximately 40-55 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by NL from This antibody was used in WB (1/500)This antibody was used in WB (1/500), very good results, Published data
Date published: 2016-05-06
Rated 3 out of 5 by HC from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-03-19
Rated 4 out of 5 by JL from Positive immunofluorescence staining of methanolPositive immunofluorescence staining of methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by LM from Strong blownout band in MCF7 cells on WBStrong blownout band in MCF7 cells on WB. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by AB from Single clean band in ASingle clean band in A-431 cells. -SCBT QC
Date published: 2015-02-25
Rated 4 out of 5 by SF from Good cytoplasmic staining with M20 on HeLa samplesGood cytoplasmic staining with M20 on HeLa samples.
Date published: 2015-01-18
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Cytokeratin 8 抗体 (M20) is rated 4.3 out of 5 by 6.
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