Date published: 2025-9-6

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Cytokeratin 8 抗体 (C51): sc-8020

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说明书
  • Cytokeratin 8抗体(C51)是小鼠单克隆IgG1 κ, 在62篇文献中引用,规格为200 µg/ml
  • raised against a Cytoskeleton preparation of HeLa cells
  • 抗-Cytokeratin 8 抗体 (C51) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Cytokeratin 8
  • 抗Cytokeratin 8抗体(C51)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 8 (C51): sc-8020免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Cytokeratin 8 抗体 (C51) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cytokeratin 8 抗体 (C51) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

细胞角蛋白8抗体(C51)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过多种应用检测小鼠、大鼠和人类样本中的细胞角蛋白8,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。抗细胞角蛋白8抗体(C51)有非结合型和多种结合型,如琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和各种Alexa Fluor®结合物。细胞角蛋白8是多种中间丝蛋白的一部分,在维持上皮细胞结构完整性方面发挥着关键作用,对于细胞分化和组织分化至关重要。细胞角蛋白8主要位于简单上皮细胞的细胞质中,在那里细胞角蛋白8形成了一个网络,提供机械支撑和抗应激能力。细胞角蛋白8的正确定位至关重要,因为表达错误会导致包括各种癌症在内的病理状况。细胞角蛋白8的表达通常在上皮来源的肿瘤中上调,这使得细胞角蛋白8成为癌症诊断和预后的重要标志物。鳞状细胞癌中细胞角蛋白8的存在与不良的临床结果有关,这凸显了其作为治疗靶标的潜力。细胞角蛋白8和18对通常在简单上皮细胞中表达,而在分层上皮细胞中的异常表达可能表明恶性肿瘤,这进一步强调了其在癌症研究和治疗策略中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 8 抗体 (C51) 参考文献:

  1. 检测非小细胞肺癌细胞系中作为 CA19-9 载体蛋白的大分子量细胞角蛋白 8。  |  Fujita, J., et al. 1999. Br J Cancer. 81: 769-73. PMID: 10555744
  2. 分层上皮细胞中细胞角蛋白 8/18 细丝形成的意义:诱导转化表型。  |  Raul, U., et al. 2004. Int J Cancer. 111: 662-8. PMID: 15252834
  3. 细胞角蛋白 8 与肿瘤细胞质膜的外叶结合。  |  Gires, O., et al. 2005. Biochem Biophys Res Commun. 328: 1154-62. PMID: 15707998
  4. 细胞角蛋白 8/18 的表达表明口腔鳞状细胞癌的预后较差。  |  Fillies, T., et al. 2006. BMC Cancer. 6: 10. PMID: 16412231
  5. CK8 与头颈部白斑病和癌的恶性程度有关。  |  Gires, O., et al. 2006. Biochem Biophys Res Commun. 343: 252-9. PMID: 16540085
  6. 人类鼻咽癌细胞中细胞角蛋白8的沉默导致顺铂敏感性增强。  |  Wang, Y., et al. 2008. Cancer Lett. 265: 188-96. PMID: 18353540
  7. 细胞角蛋白8在人类角膜结膜上皮中表达,特别是在角膜缘上皮细胞中。  |  Merjava, S., et al. 2011. Invest Ophthalmol Vis Sci. 52: 787-94. PMID: 20926822
  8. 简单上皮中细胞角蛋白的表达。III.通过 cDNA 序列体外和原位杂交检测正常细胞和肿瘤细胞中编码人类细胞角蛋白第 8 号和第 18 号的 mRNA。  |  Leube, RE., et al. 1986. Differentiation. 33: 69-85. PMID: 2434381
  9. 甲状腺无节细胞癌中的细胞角蛋白-8:不仅仅是一种简单的细胞骨架结构蛋白  |  Guo, D., et al. 2018. Int J Mol Sci. 19: PMID: 29443941
  10. 评估健康人和前列腺癌患者细胞角蛋白 8 和 18 片段的新型肿瘤标记物。  |  Silén, A., et al. 1994. Prostate. 24: 326-32. PMID: 7516071
  11. 用于定量检测健康人和癌症患者血清中细胞角蛋白 8 和 18 片段的新型 IRMA 和 ELISA。  |  Silén, A., et al. 1995. Scand J Clin Lab Invest. 55: 153-61. PMID: 7545308
  12. 正常和(恶性前)头颈部上皮细胞角蛋白的表达:综述。  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 8 抗体 (C51)

sc-8020
200 µg/ml
RMB2377.00

Cytokeratin 8 (C51): m-IgG Fc BP-HRP 套装

sc-528204
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 8 (C51): m-IgGκ BP-HRP 套装

sc-520526
200 µg Ab, 40 µg BP
RMB2662.00

Cytokeratin 8 (C51): m-IgG1 BP-HRP 套装

sc-542814
200 µg Ab; 20 µg BP
RMB2662.00

Cytokeratin 8 抗体 (C51) AC

sc-8020 AC
500 µg/ml, 25% agarose
RMB3129.00

Cytokeratin 8 抗体 (C51) HRP

sc-8020 HRP
200 µg/ml
RMB2377.00

Cytokeratin 8 抗体 (C51) FITC

sc-8020 FITC
200 µg/ml
RMB2482.00

Cytokeratin 8 抗体 (C51) PE

sc-8020 PE
200 µg/ml
RMB2580.00

Cytokeratin 8 抗体 (C51) Alexa Fluor® 488

sc-8020 AF488
200 µg/ml
RMB2685.00

Cytokeratin 8 抗体 (C51) Alexa Fluor® 546

sc-8020 AF546
200 µg/ml
RMB2685.00

Cytokeratin 8 抗体 (C51) Alexa Fluor® 594

sc-8020 AF594
200 µg/ml
RMB2685.00

Cytokeratin 8 抗体 (C51) Alexa Fluor® 647

sc-8020 AF647
200 µg/ml
RMB2685.00

Cytokeratin 8 抗体 (C51) Alexa Fluor® 680

sc-8020 AF680
200 µg/ml
RMB2685.00

Cytokeratin 8 抗体 (C51) Alexa Fluor® 790

sc-8020 AF790
200 µg/ml
RMB2685.00

What is the proper antigen retrieval condition suitable for this antibody when staining on FFPE-section?

Asked by: Anonymous
Thank you for your question. You can find our standard protocol for IHC(P) applications by following this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining The recommended antigen retrieval method is heat treatment, which in this protocol is listed under point "C. Formalin-Fixed, Paraffin-Embedded Tissue Sections"
Answered by: Tech Support Europe
Date published: 2020-10-30

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-8020 PE), fluorescein (sc-8020 FITC), Alexa Fluor® 488 (sc-8020 AF488) or Alexa Fluor® 647 (sc-8020 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-18
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Rated 4 out of 5 by from Good antibodyWorks as we expected. Tested by immunofluorescence.
Date published: 2018-12-18
Rated 5 out of 5 by from Western效果不错。Western目的条带:最下面的条带,ladder的45KD附近,条带非常清晰。按照说明书建议浓度使用。
Date published: 2017-10-16
Rated 4 out of 5 by from Outstanding !!Superb; antibodies don't get any better; great WB, IF and IHC !!
Date published: 2017-01-14
Rated 5 out of 5 by from Great antibody for WBThe Cytokeratin 8 Antibody (C51): sc-8020 shows strong signal with minimal background in Western blot applications. This antibody has been cited over 20 times for further verification.
Date published: 2017-01-10
Rated 5 out of 5 by from nice performancevery specific staining in human prostate tissue in IHC
Date published: 2016-10-28
Rated 5 out of 5 by from very goodI used this antibody for human prostate tissue IHC staining, very specific signal
Date published: 2016-10-27
Rated 5 out of 5 by from Strong bands in WBStrong bands in WB, happy with purchase
Date published: 2015-04-22
Rated 5 out of 5 by from Great IHC data in paraffinGreat IHC data in paraffin-embedded human liver. -SCBT QC
Date published: 2015-03-11
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Cytokeratin 8 抗体 (C51) is rated 4.8 out of 5 by 11.
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