Date published: 2025-9-6
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Cytokeratin 7 Antibody (RCK105) - Western Blotting - Image 356323
细胞角蛋白 7 抗体 (RCK105): sc-23876. Hep G2 (A), RIN-m5F (B) 和 MDCK (C) 全细胞裂解液中细胞角蛋白 7 表达的 Western 印迹分析.
细胞角蛋白 7 抗体 (RCK105): sc-23876. Hep G2 (A), RIN-m5F (B) 和 MDCK (C) 全细胞裂解液中细胞角蛋白 7 表达的 Western 印迹分析.

Cytokeratin 7 抗体 (RCK105): sc-23876

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说明书
  • Cytokeratin 7 抗体 RCK105 是小鼠单克隆 IgG1 κ，Cytokeratin 7抗体, 在52篇文献中引用，规格为200 µg/ml
  • 针对T24细胞
  • Cytokeratin 7 抗体 (RCK105) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat, human, hamster, canine 和porcine 来源的 Cytokeratin 7
  • 抗Cytokeratin 7抗体（RCK105）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 7 (RCK105): sc-23876的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Cytokeratin 7 抗体 (RCK105) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cytokeratin 7 抗体 (RCK105) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cytokeratin 抗体 包括 Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17和Cytokeratin 18

细胞角蛋白7抗体（RCK105）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对细胞角蛋白7（也称为KRT7或肌动蛋白）设计。抗细胞角蛋白7抗体（RCK105）针对T24细胞产生，对多种物种（包括小鼠、大鼠、人、仓鼠、犬和猪）均具有广泛的反应性，因此适用于多种应用，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）。细胞角蛋白7是一种位于上皮细胞细胞骨架中的中间丝蛋白，在维持细胞结构完整性和促进组织分化与特化方面发挥着关键作用。细胞角蛋白7在细胞内的精确定位对于上皮组织的稳定性和弹性至关重要，细胞角蛋白7是识别和鉴定正常和恶性上皮细胞的重要标记物。抗细胞角蛋白7抗体（RCK105）使研究人员能够有效地研究上皮细胞生物学，监测分化过程，并研究各种癌症的分子机制。细胞角蛋白7（RCK105）单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）以及一系列Alexa Fluor®结合物，可满足各种实验需求。此外，细胞角蛋白7在上皮细胞的结构框架中起着重要作用，通过突出特定的细胞结构，有助于肿瘤的诊断和分类。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 7 抗体 (RCK105) 参考文献:

  1. 角蛋白 7 单克隆抗体在腺癌鉴别诊断中的应用。  |  Ramaekers, F., et al. 1990. Am J Pathol. 136: 641-55. PMID: 1690512
  2. 7 号角蛋白的单克隆抗体：特征和在肿瘤诊断中的价值。  |  Vojtĕsek, B., et al. 1990. Neoplasma. 37: 333-42. PMID: 1695327
  3. 14 种新的角蛋白单克隆抗体系列：特性和在组织病理学诊断中的价值。  |  Bártek, J., et al. 1991. J Pathol. 164: 215-24. PMID: 1716305
  4. 表皮瘤中简单上皮角蛋白 8 和 18 的表达。  |  Markey, AC., et al. 1991. J Invest Dermatol. 97: 763-70. PMID: 1717607
  5. 常规固定的石蜡包埋人体组织中角蛋白 7 的免疫组化显示。  |  van Niekerk, CC., et al. 1991. J Pathol. 165: 145-52. PMID: 1720817
  6. 分化皮肤中的角蛋白抗原。  |  Lane, EB., et al. 1985. Ann N Y Acad Sci. 455: 241-58. PMID: 2417515
  7. 使用针对单个角蛋白多肽的特异性单克隆抗体对胃肠癌进行鉴别诊断。  |  Osborn, M., et al. 1986. Lab Invest. 55: 497-504. PMID: 2429069
  8. 核梭杆菌通过调节KRT7-AS/KRT7促进结直肠癌转移。  |  Chen, S., et al. 2020. Gut Microbes. 11: 511-525. PMID: 31910722
  9. 癌细胞中的细胞角蛋白7和细胞角蛋白20表达：对15424例癌症的组织微阵列研究。  |  Dum, D., et al. 2022. Exp Mol Pathol. 126: 104762. PMID: 35390310
  10. 评估细胞角蛋白-7和细胞角蛋白-19表达与前列腺腺癌格里森评分的关系。  |  Soltanipur, M., et al. 2023. Iran J Pathol. 18: 363-369. PMID: 37942200
  11. 人类细胞角蛋白目录：正常上皮, 肿瘤和培养细胞中的表达模式。  |  Moll, R., et al. 1982. Cell. 31: 11-24. PMID: 6186379
  12. 角蛋白与皮肤  |  Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 7 抗体 (RCK105)

sc-23876
200 µg/ml
RMB2377.00

Cytokeratin 7 (RCK105): m-IgG Fc BP-HRP 套装

sc-526622
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 7 (RCK105): m-IgG1 BP-HRP 套装

sc-531995
200 µg Ab; 20 µg BP
RMB2662.00

Cytokeratin 7 抗体 (RCK105) AC

sc-23876 AC
500 µg/ml, 25% agarose
RMB3129.00

Cytokeratin 7 抗体 (RCK105) HRP

sc-23876 HRP
200 µg/ml
RMB2377.00

Cytokeratin 7 抗体 (RCK105) FITC

sc-23876 FITC
200 µg/ml
RMB2482.00

Cytokeratin 7 抗体 (RCK105) PE

sc-23876 PE
200 µg/ml
RMB2580.00

Cytokeratin 7 抗体 (RCK105) Alexa Fluor® 488

sc-23876 AF488
200 µg/ml
RMB2685.00

Cytokeratin 7 抗体 (RCK105) Alexa Fluor® 546

sc-23876 AF546
200 µg/ml
RMB2685.00

Cytokeratin 7 抗体 (RCK105) Alexa Fluor® 594

sc-23876 AF594
200 µg/ml
RMB2685.00

Cytokeratin 7 抗体 (RCK105) Alexa Fluor® 647

sc-23876 AF647
200 µg/ml
RMB2685.00

Cytokeratin 7 抗体 (RCK105) Alexa Fluor® 680

sc-23876 AF680
200 µg/ml
RMB2685.00

Cytokeratin 7 抗体 (RCK105) Alexa Fluor® 790

sc-23876 AF790
200 µg/ml
RMB2685.00

请问Cytokeratin 7 抗体 (RCK105) FITC抗体是自带荧光可以染流式的是吗？不需要接二抗

Asked by: wj921027
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Germaine
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 1 out of 5 by from Anticorpo non performante.Purtroppo l'anticorpo non ha performato bene. Abbiamo applicato le seguenti condizioni: 1:50 ph6 e ph9.
Date published: 2021-05-06
Rated 5 out of 5 by from Excellent resultsI tried this antibody on FFPE equine haired skin, using a standard protocol (1:50; pH 9.0). The staining was specific and only apocrine glands were positive.
Date published: 2020-05-05
Rated 4 out of 5 by from Good in IFWorks well in IF(P), not so much in IHC. Try to avoid acidic antigen retrieval.
Date published: 2018-02-20
Rated 5 out of 5 by from Worked well on FFPEI used this antibody on formalin-fixed paraffin-embedded tissues at a dilution of 1:30 for 15 minutes. It worked well; glandular tissues were appropriately identified.
Date published: 2017-08-10
Rated 5 out of 5 by from Works well on mouse sample and placenta1/500 dilution was used for WB. All lane were very clear .
Date published: 2017-06-30
Rated 5 out of 5 by from Great!I tried a sample to do the IHC experiment. It works well.
Date published: 2017-06-28
Rated 5 out of 5 by from Specific for IHC on formalin-fixed tissue of pighighly specific staining but only medium sensitivity, require heat-induced antigen retrieval with Tris-EDTA pH 9, primary antibody dilution of 1:50, and signal amplification by using avidin-biotin-complex
Date published: 2017-04-08
Rated 5 out of 5 by from Cytokeratin 7 is good for IHC stainningWe used this product to stain bile duct in mouse liver with IHC.Finally,exact results were shown.
Date published: 2017-02-16
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Cytokeratin 7 抗体 (RCK105) is rated 4.6 out of 5 by 17.
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