Date published: 2025-9-6
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Cytokeratin 5 Antibody (RCK103) - Western Blotting - Image 9889
细胞角蛋白 5 抗体 (RCK103): sc-32721. 对 HeLa 全细胞裂解液中细胞角蛋白 5 的表达进行 Western 印迹分析.
细胞角蛋白 5 抗体 (RCK103): sc-32721. 对 HeLa 全细胞裂解液中细胞角蛋白 5 的表达进行 Western 印迹分析.

Cytokeratin 5 抗体 (RCK103): sc-32721

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说明书
  • Cytokeratin 5 抗体 RCK103 是小鼠单克隆 IgG1 κ，Cytokeratin 5抗体, 在25篇文献中引用，规格为200 µg/ml
  • 针对来源于human的细胞角蛋白
  • Cytokeratin 5 抗体 (RCK103) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Cytokeratin 5
  • 抗Cytokeratin 5抗体（RCK103）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 5 (RCK103): sc-32721的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Cytokeratin 5 抗体 (RCK103)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cytokeratin 抗体 包括 Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17和Cytokeratin 18

细胞角蛋白5抗体（RCK103）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的细胞角蛋白5蛋白。抗细胞角蛋白5抗体（RCK103）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。细胞角蛋白5是上皮细胞细胞骨架的重要组成部分，主要位于分层上皮的基底层，在提供机械支撑和细胞应激恢复方面发挥着关键作用。这种结构定位对于维持上皮组织的完整性至关重要，特别是在皮肤和呼吸道等易受摩擦和磨损的区域。细胞角蛋白5的表达可以作为某些上皮肿瘤的标志物，有助于诊断和定性恶性肿瘤。此外，细胞角蛋白5基因（KRT5）的突变与常染色体显性遗传病表皮松解性大疱症有关，这凸显了细胞角蛋白5在正常生理和疾病状态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 5 抗体 (RCK103) 参考文献:

  1. 细胞角蛋白5阳性细胞代表上皮性卵巢癌中的耐药亚群  |  Corr, BR., et al. 2015. Int J Gynecol Cancer. 25: 1565-73. PMID: 26495758
  2. 细胞角蛋白5决定乳腺肌上皮细胞的成熟。  |  Deckwirth, V., et al. 2021. iScience. 24: 102413. PMID: 34007958
  3. 细胞角蛋白5和细胞角蛋白20与Ta非肌肉浸润性膀胱癌的肿瘤分级呈负相关。  |  Muilwijk, T., et al. 2021. J Cell Mol Med. 25: 7890-7900. PMID: 34184816
  4. 细胞角蛋白5和细胞角蛋白6在正常组织和癌组织中的表达无关，具有不同的诊断意义。  |  Völkel, C., et al. 2022. Virchows Arch. 480: 433-447. PMID: 34559291
  5. 细胞角蛋白5阳性肺腺癌的临床病理特征。  |  Terada, K., et al. 2023. Histopathology. 82: 439-453. PMID: 36239561
  6. 评估健康人和前列腺癌患者细胞角蛋白 8 和 18 片段的新型肿瘤标记物。  |  Silén, A., et al. 1994. Prostate. 24: 326-32. PMID: 7516071
  7. 单纯性表皮松解症：角蛋白 5 突变是表皮细胞骨架功能的完全显性等位基因。  |  Stephens, K., et al. 1995. Am J Hum Genet. 56: 577-85. PMID: 7534039
  8. 用于定量检测健康人和癌症患者血清中细胞角蛋白 8 和 18 片段的新型 IRMA 和 ELISA。  |  Silén, A., et al. 1995. Scand J Clin Lab Invest. 55: 153-61. PMID: 7545308
  9. 正常和（恶性前）头颈部上皮细胞角蛋白的表达：综述。  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
  10. 肺癌患者血清和组织中细胞角蛋白 19（cyfra 21-1）片段的分布。  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
  11. 通过反义 RNA 使 p53 功能失活可诱导肺癌细胞的侵袭能力并下调细胞角蛋白的合成。  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
  12. 肝细胞中细胞角蛋白的表达, 纤维组织和微妙功能。  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 5 抗体 (RCK103)

sc-32721
200 µg/ml
$316.00

Cytokeratin 5 抗体 (RCK103) AC

sc-32721 AC
500 µg/ml, 25% agarose
$416.00

Cytokeratin 5 抗体 (RCK103) HRP

sc-32721 HRP
200 µg/ml
$316.00

Cytokeratin 5 抗体 (RCK103) FITC

sc-32721 FITC
200 µg/ml
$330.00

Cytokeratin 5 抗体 (RCK103) PE

sc-32721 PE
200 µg/ml
$343.00

Cytokeratin 5 抗体 (RCK103) Alexa Fluor® 488

sc-32721 AF488
200 µg/ml
$357.00

Cytokeratin 5 抗体 (RCK103) Alexa Fluor® 546

sc-32721 AF546
200 µg/ml
$357.00

Cytokeratin 5 抗体 (RCK103) Alexa Fluor® 594

sc-32721 AF594
200 µg/ml
$357.00

Cytokeratin 5 抗体 (RCK103) Alexa Fluor® 647

sc-32721 AF647
200 µg/ml
$357.00

Cytokeratin 5 抗体 (RCK103) Alexa Fluor® 680

sc-32721 AF680
200 µg/ml
$357.00

Cytokeratin 5 抗体 (RCK103) Alexa Fluor® 790

sc-32721 AF790
200 µg/ml
$357.00

For flow, is it a intracellular marker or surface marker?

Asked by: jason 2
Thank you for your question. This antibody stains intracellular. Please contact Technical Service if you have any further questions.
Answered by: Tech Service
Date published: 2023-02-04

Is antigen retrieval necessary when using this antibody in IHC(P)?

Asked by: AbPolly
Thank you for your question. We do recommend antigen retrieval when working with paraffin-embedded sections, specifically sodium citrate buffer, pH 6.0, and heat. Our full protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service
Date published: 2017-02-03
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Rated 5 out of 5 by from cytokeratin 5Cells were fixed in 4% PFA at room temperature (RT) for 15 min, and permeabilized in 0.2% Triton X-100 at RT for 20 min. Cytokeratin 5 antibody was used 1/100 diluted in FACS buffer, supplemented with 60µg/ml human IgG) for overnight at 8°C. The cells were washed and analyzed by flow cytometry.
Date published: 2023-01-16
Rated 4 out of 5 by from Good antibodyThe antibody showed a specific immunostaining in equine haired skin. I used a standard protocol (1:50; pH 9.0).
Date published: 2020-05-05
Rated 5 out of 5 by from Good antibodyI applied for the free sample of cytokeratin 5 antibody (SC-32721) last month, immunohistochemical staining of chicken skin paraffin section indicate that the antibody has a good immunoreactivity on keratinocytes
Date published: 2017-08-19
Rated 5 out of 5 by from Great antobodyThe antibody works well, and the band is clear, the position is specific
Date published: 2017-03-22
Rated 5 out of 5 by from Great for WB, stainingReliable antibody that produces good bands in WB and strong staining. Recommended for mouse, rat, and human Cytokeratin 5.
Date published: 2017-02-03
Rated 5 out of 5 by from Good resultgood IHC result from human prostate tissue staining
Date published: 2016-10-28
Rated 5 out of 5 by from excellent performancegood antibody for IHC , I used it for human tissue staining
Date published: 2016-10-27
Rated 5 out of 5 by from Excellent staining in IF with PFA fixed humanExcellent staining in IF with PFA fixed human lung samples -SCBT Publication Review
Date published: 2015-03-28
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Cytokeratin 5 抗体 (RCK103) is rated 4.8 out of 5 by 11.
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