Date published: 2025-9-6
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Cytokeratin 20 Antibody (E-9) - Western Blotting - Image 381323
细胞角蛋白 20 抗体 (E-9): sc-271183. 细胞角蛋白 20 在 HT-29 (A), HCT-116 (B), A-10 (C), P19 (D), NIH/3T3 (E) 和 F9 (F) 全细胞裂解液中表达的 Western 印迹分析.
细胞角蛋白 20 抗体 (E-9): sc-271183. 细胞角蛋白 20 在 HT-29 (A), HCT-116 (B), A-10 (C), P19 (D), NIH/3T3 (E) 和 F9 (F) 全细胞裂解液中表达的 Western 印迹分析.

Cytokeratin 20 抗体 (E-9): sc-271183

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说明书
  • Cytokeratin 20抗体(E-9)是小鼠单克隆IgG1 λ Cytokeratin 20抗体, 在4篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Cytokeratin 20的N-terminus附近的氨基酸39-74之间
  • Cytokeratin 20 抗体 (E-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Cytokeratin 20
  • 抗Cytokeratin 20抗体（E-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 20 (E-9): sc-271183的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Cytokeratin 20 Antibody (E-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Cytokeratin 20 Antibody (E-9) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Cytokeratin抗体，包括Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 18, Cytokeratin 19, Cytokeratin 20, Cytokeratin 23, Cytokeratin 2E, Cytokeratin 2p和Cytokeratin 3

细胞角蛋白20抗体（E-9）是一种IgG1 λ小鼠单克隆细胞角蛋白20抗体（也被称为KRT20抗体或角蛋白20抗体），能够通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC-P）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人的细胞角蛋白20蛋白。细胞角蛋白20抗体（E-9）既提供非偶联的抗细胞角蛋白20抗体形式，也提供多种偶联形式的抗细胞角蛋白20抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。细胞角蛋白是一组多样化的中间丝蛋白（IFPs），在角质化和非角质化上皮组织中均以成对的形式表达，在脊椎动物表皮中它们构成多达85%的成熟角质细胞。细胞角蛋白在分化和组织特化中起着关键作用，并维持上皮细胞的整体结构完整性。α-螺旋状卷曲螺旋二聚体在侧面以端对端的方式结合，形成直径为10 nm的细丝。细胞角蛋白是组织分化的有用标志物，此外，它们还有助于恶性肿瘤的鉴定。细胞角蛋白20在胃肠道的杯状细胞和肠上皮细胞中大量表达，是胰腺癌和结直肠癌的有用标志物。细胞角蛋白20还有助于区分不同类型的高度相关的癌，如肾嗜酸细胞瘤和肾细胞癌。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 20 抗体 (E-9) 参考文献:

  1. 胰腺癌和结直肠癌中细胞角蛋白 20 表达的特征。  |  Wildi, S., et al. 1999. Clin Cancer Res. 5: 2840-7. PMID: 10537351
  2. 使用细胞角蛋白 20 和甲状腺转录因子 1 的抗体可将梅克尔细胞癌与转移性小细胞癌区分开来。  |  Leech, SN., et al. 2001. J Clin Pathol. 54: 727-9. PMID: 11533085
  3. 结直肠癌患者血液中的细胞角蛋白20阳性细胞是一种不良预后标志物。  |  Kust, D., et al. 2016. Acta Clin Belg. 71: 235-43. PMID: 27144776
  4. 癌细胞中的细胞角蛋白7和细胞角蛋白20表达：对15424例癌症的组织微阵列研究。  |  Dum, D., et al. 2022. Exp Mol Pathol. 126: 104762. PMID: 35390310
  5. 细胞角蛋白20和p53作为尿液细胞学辅助免疫细胞标记物在检测尿路上皮癌中的诊断意义：系统回顾性研究。  |  Angel, E. 2023. Iran J Pathol. 18: 257-269. PMID: 37942190
  6. 作为卵巢转移癌诊断标志物的角蛋白 7 和 20。  |  Wauters, CC., et al. 1995. Hum Pathol. 26: 852-5. PMID: 7543441
  7. 正常和（恶性前）头颈部上皮细胞角蛋白的表达：综述。  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
  8. 编码细胞角蛋白 20 的人类基因及其在胎儿发育过程中和胃肠癌中的表达。  |  Moll, R., et al. 1993. Differentiation. 53: 75-93. PMID: 8359595
  9. 肺癌患者血清和组织中细胞角蛋白 19（cyfra 21-1）片段的分布。  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
  10. 角蛋白与皮肤  |  Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554
  11. 通过反义 RNA 使 p53 功能失活可诱导肺癌细胞的侵袭能力并下调细胞角蛋白的合成。  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
  12. 肝细胞中细胞角蛋白的表达, 纤维组织和微妙功能。  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 20 抗体 (E-9)

sc-271183
200 µg/ml
RMB2377.00

Cytokeratin 20 (E-9): m-IgG Fc BP-HRP 套装

sc-551987
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 20 抗体 (E-9) AC

sc-271183 AC
500 µg/ml, 25% agarose
RMB3129.00

Cytokeratin 20 抗体 (E-9) HRP

sc-271183 HRP
200 µg/ml
RMB2377.00

Cytokeratin 20 抗体 (E-9) FITC

sc-271183 FITC
200 µg/ml
RMB2482.00

Cytokeratin 20 抗体 (E-9) PE

sc-271183 PE
200 µg/ml
RMB2580.00

Cytokeratin 20 抗体 (E-9) Alexa Fluor® 488

sc-271183 AF488
200 µg/ml
RMB2685.00

Cytokeratin 20 抗体 (E-9) Alexa Fluor® 546

sc-271183 AF546
200 µg/ml
RMB2685.00

Cytokeratin 20 抗体 (E-9) Alexa Fluor® 594

sc-271183 AF594
200 µg/ml
RMB2685.00

Cytokeratin 20 抗体 (E-9) Alexa Fluor® 647

sc-271183 AF647
200 µg/ml
RMB2685.00

Cytokeratin 20 抗体 (E-9) Alexa Fluor® 680

sc-271183 AF680
200 µg/ml
RMB2685.00

Cytokeratin 20 抗体 (E-9) Alexa Fluor® 790

sc-271183 AF790
200 µg/ml
RMB2685.00

Cytokeratin 20 (E-9) 中和勝肽

sc-271183 P
100 µg/0.5 ml
RMB511.00

Hello. I would like to know what the secondary antibody for this antibody to use for immunohistochemistory (DAB) is. I may stain cytokeratin in mouse tissue, so if this secondary antibody should be anti-mouse one, it is not good. We hope anti-goat one.

Asked by: Yoshi
Thank you for your question.Cytokeratin 20 Antibody (E-9) is a mouse monoclonal IgG1 λ.It can be used for IHC experiments on mouse tissues. If you choose the secondary antibody you should choose anti-mouse secondary antibody. We recommend you to use m-IgG1 BP-HRP: sc-525408. Thank you.
Answered by: Jiajun Z
Date published: 2024-07-11

What application is the blocking peptide sc-271183 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works on mouse but not on canine tissuesWe tested it on canine intestine and stomach, but the antibody did not show immunolabeling, unfortunately, despite the high protein similarity (tested by protein alignment).
Date published: 2025-04-04
Rated 3 out of 5 by from Weak signal with IHCWe tested this antibody on formalin-fixed paraffin-embedded human colon metastasis and had a weak positive signal with low background.
Date published: 2025-03-24
Rated 3 out of 5 by from Good, specific stainingIF staining was performed for Merkel cells in mouse skin. The staining was specific whereas weaker (showing much less CK20+ cells than TROMA-1 staining).
Date published: 2018-02-13
Rated 5 out of 5 by from Positive for Human, Mouse & RatWe received this as a sample. Got this to work on 3 species, mouse, rat and human colon. Very easy and great results. I will be buying the product for years.
Date published: 2017-10-23
Rated 5 out of 5 by from 质量不错一个月前订购的该产品，发货很快，销售服务也很不错，及时联系送货事宜，抗体效价挺好，还会再选用santa cruz的抗体！
Date published: 2017-08-17
Rated 4 out of 5 by from CK20 antibody in Western BlotDetected CK20 in both mouse and human colorectal cancer cell lines by Western blot.
Date published: 2017-07-24
Rated 5 out of 5 by from Good specificity and signal strength in T84Good specificity and signal strength in T84 whole cell lysate. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Good cytoplasmic staining with paraffinGood cytoplasmic staining with paraffin-embedded human small intestine tissue -SCBT QC
Date published: 2015-03-05
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Cytokeratin 20 抗体 (E-9) is rated 4.4 out of 5 by 9.
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