Date published: 2025-9-6
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Cytokeratin 19 Antibody (A53-B/A2) - Western Blotting - Image 149546
细胞角蛋白 19 抗体 (A53-B/A2): sc-6278. MCF7 (A), MIA PaCa-2 (B) 和 MDA-MB-231 (C) 全细胞裂解液中细胞角蛋白 19 表达的 Western 印迹分析.
细胞角蛋白 19 抗体 (A53-B/A2): sc-6278. MCF7 (A), MIA PaCa-2 (B) 和 MDA-MB-231 (C) 全细胞裂解液中细胞角蛋白 19 表达的 Western 印迹分析.

Cytokeratin 19 抗体 (A53-B/A2): sc-6278

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说明书
  • Cytokeratin 19抗体(A53-B/A2)是小鼠单克隆IgG2a κ, 在97篇文献中引用，规格为200 µg/ml
  • 针对human来源的乳腺癌细胞系MCF7
  • Cytokeratin 19 抗体 (A53-B/A2) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 amino acids 312-335 of Cytokeratin 19
  • 抗Cytokeratin 19抗体（A53-B/A2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 19 (A53-B/A2): sc-6278的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Cytokeratin 19 抗体 (A53-B/A2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cytokeratin 19 抗体 (A53-B/A2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cytokeratin 抗体 包括 Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17和Cytokeratin 18

细胞角蛋白19抗体（A53-B/A2）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测人类细胞角蛋白19。抗细胞角蛋白19抗体（A53-B/A2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。细胞角蛋白19是细胞角蛋白家族的一员，主要位于鳞状上皮的上皮层，在维持上皮细胞的完整结构和机械弹性方面发挥着重要作用。这种定位使细胞角蛋白19能够在细胞压力和损伤期间有效发挥作用，为上皮组织整体健康提供支持。细胞角蛋白19的表达在各种恶性肿瘤中通常会上调，因此细胞角蛋白19成为诊断肝癌和肺癌等疾病的宝贵生物标记物。血清中细胞角蛋白19片段水平升高与肿瘤存在有关，这凸显了其在临床诊断中的重要性。细胞角蛋白19作为原位癌和癌前病变标志物的功能进一步凸显其在癌症研究和病理学中的重要性，为肿瘤分化和进展提供了新的见解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 19 抗体 (A53-B/A2)

sc-6278
200 µg/ml
RMB2377.00

Cytokeratin 19 (A53-B/A2): m-IgG Fc BP-HRP 套装

sc-525430
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 19 (A53-B/A2): m-IgG2a BP-HRP 套装

sc-546252
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 19 抗体 (A53-B/A2) AC

sc-6278 AC
500 µg/ml, 25% agarose
RMB3129.00

Cytokeratin 19 抗体 (A53-B/A2) HRP

sc-6278 HRP
200 µg/ml
RMB2377.00

Cytokeratin 19 抗体 (A53-B/A2) FITC

sc-6278 FITC
200 µg/ml
RMB2482.00

Cytokeratin 19 抗体 (A53-B/A2) PE

sc-6278 PE
200 µg/ml
RMB2580.00

Cytokeratin 19 抗体 (A53-B/A2) Alexa Fluor® 488

sc-6278 AF488
200 µg/ml
RMB2685.00

Cytokeratin 19 抗体 (A53-B/A2) Alexa Fluor® 546

sc-6278 AF546
200 µg/ml
RMB2685.00

Cytokeratin 19 抗体 (A53-B/A2) Alexa Fluor® 594

sc-6278 AF594
200 µg/ml
RMB2685.00

Cytokeratin 19 抗体 (A53-B/A2) Alexa Fluor® 647

sc-6278 AF647
200 µg/ml
RMB2685.00

Cytokeratin 19 抗体 (A53-B/A2) Alexa Fluor® 680

sc-6278 AF680
200 µg/ml
RMB2685.00

Cytokeratin 19 抗体 (A53-B/A2) Alexa Fluor® 790

sc-6278 AF790
200 µg/ml
RMB2685.00

Which antigen retrieval technique is needed for IHC-P ? thanks

Asked by: SophiePaquet
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval.
Answered by: Technical Support
Date published: 2019-06-16

What is the recommended secondary reagent to use with this mouse monoclonal primary antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-11

Can this antibody be used for detection of Cytokeratin 19 of mouse origin?

Asked by: AbPolly
Thank you for your question. This antibody has not been validated for use to detect Cytokeratin 19 of mouse origin. For further details and sequence analysis, please contact our Technical Service Department.
Answered by: Technical Support 5
Date published: 2017-01-11
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Rated 5 out of 5 by from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from Works wellUsed the antibody for IP and WB and worked well in both cases
Date published: 2020-03-09
Rated 5 out of 5 by from Good on FFPEI used this antibody on formalin-fixed paraffin-embedded human carcinomas. The staining was quite decent. The antibody was used at a 1:30 dilution and incubated for 15 minutes. Staining was clear. I also used CK19 (A-3) antibody on a serial section simultaneously and although both antibodies were quite good, the staining from CK19 (A-3) was more vibrant.
Date published: 2017-08-10
Rated 5 out of 5 by from Just a great productStrong western blot data; also a superb product for western applications
Date published: 2017-01-29
Rated 5 out of 5 by from Good antibodyThe Cytokeratin 19 Antibody (A53-B/A2): sc-6278 works very well for IHC with formalin fixed, paraffin-embedded tissue sections showing localized staining to the cytoplasm and membrane. This antibody has also been cited over 40 times for further verification.
Date published: 2017-01-16
Rated 5 out of 5 by from IHC publication with human breast cancerIHC publication with human breast cancer. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Beautiful IHC data in paraffinBeautiful IHC data in paraffin-embedded human breast and pancreas tissue. -SCBT QC
Date published: 2015-02-17
Rated 5 out of 5 by from Immunofluorescence publication with human liverImmunofluorescence publication with human liver. -SCBT Publication Review
Date published: 2015-02-10
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Cytokeratin 19 抗体 (A53-B/A2) is rated 5.0 out of 5 by 10.
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