Date published: 2025-9-6

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Cytokeratin 16 抗体 (LL025): sc-53255

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说明书
  • Cytokeratin 16 抗体 LL025 是小鼠单克隆 IgG1 κ,Cytokeratin 16抗体, 在19篇文献中引用,规格为200 µg/ml
  • 与human起源的Cytokeratin 16的C-terminal合成肽比对
  • Cytokeratin 16 抗体 (LL025) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 Cytokeratin 16
  • 抗Cytokeratin 16抗体(LL025)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 16 (LL025): sc-53255免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Cytokeratin 16 抗体 (LL025) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cytokeratin 16 抗体 (LL025) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

细胞角蛋白16抗体(LL025)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类来源的细胞角蛋白16蛋白。抗细胞角蛋白16抗体(LL025)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。细胞角蛋白16是细胞角蛋白家族的一员,该家族由多种中间丝蛋白组成,对于维持上皮细胞的完整结构至关重要。细胞角蛋白16(LL025)单克隆抗体主要作用于上皮细胞细胞质中的蛋白质,特别是鳞状上皮细胞,其中细胞角蛋白16在提供机械支撑和抵抗物理压力方面发挥着至关重要的作用。细胞角蛋白16是确定鳞状细胞癌肿瘤来源的标志物,可指示肿瘤分化状态。细胞角蛋白16在头颈部良性鳞状上皮和鳞状细胞癌中表达,在乳腺和汗腺管腔细胞中表达,而在非浸润性乳腺癌和正常乳腺组织中则不表达。细胞角蛋白16的突变与多种皮肤疾病有关,包括1型先天性厚皮症、非表皮松解性掌跖角化病和单侧掌跖疣状痣,这凸显了细胞角蛋白16在正常生理和疾病状态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 16 抗体 (LL025) 参考文献:

  1. [口腔畸胎囊肿的临床病理和免疫组化研究]。  |  Shen, J., et al. 2005. Zhonghua Kou Qiang Yi Xue Za Zhi. 40: 62-6. PMID: 15774156
  2. 39 例腺样牙源性肿瘤的临床病理和免疫组化研究:一项多中心研究。  |  Leon, JE., et al. 2005. Oral Oncol. 41: 835-42. PMID: 15979930
  3. 细胞角蛋白 6 和 16 经常在头颈部鳞状细胞癌细胞系和新鲜活检组织中表达。  |  Sesterhenn, AM., et al. 2005. Anticancer Res. 25: 2675-80. PMID: 16080511
  4. 人类乳腺癌中与基底细胞特异性和过度增殖相关的角蛋白  |  Wetzels, RH., et al. 1991. Am J Pathol. 138: 751-63. PMID: 1705754

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 16 抗体 (LL025)

sc-53255
200 µg/ml
RMB2377.00

Cytokeratin 16 (LL025): m-IgG Fc BP-HRP 套装

sc-526786
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 16 (LL025): m-IgG1 BP-HRP 套装

sc-532159
200 µg Ab; 20 µg BP
RMB2662.00

Cytokeratin 16 抗体 (LL025) AC

sc-53255 AC
500 µg/ml, 25% agarose
RMB3129.00

Cytokeratin 16 抗体 (LL025) HRP

sc-53255 HRP
200 µg/ml
RMB2377.00

Cytokeratin 16 抗体 (LL025) FITC

sc-53255 FITC
200 µg/ml
RMB2482.00

Cytokeratin 16 抗体 (LL025) PE

sc-53255 PE
200 µg/ml
RMB2580.00

Cytokeratin 16 抗体 (LL025) Alexa Fluor® 488

sc-53255 AF488
200 µg/ml
RMB2685.00

Cytokeratin 16 抗体 (LL025) Alexa Fluor® 546

sc-53255 AF546
200 µg/ml
RMB2685.00

Cytokeratin 16 抗体 (LL025) Alexa Fluor® 594

sc-53255 AF594
200 µg/ml
RMB2685.00

Cytokeratin 16 抗体 (LL025) Alexa Fluor® 647

sc-53255 AF647
200 µg/ml
RMB2685.00

Cytokeratin 16 抗体 (LL025) Alexa Fluor® 680

sc-53255 AF680
200 µg/ml
RMB2685.00

Cytokeratin 16 抗体 (LL025) Alexa Fluor® 790

sc-53255 AF790
200 µg/ml
RMB2685.00

I am using Cytokeratin 16 (LL025): sc-53255 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Popular for IF/IHC(P)!This mouse monoclonal Cytokeratin 16 Antibody (LL025) is a popular choice for IF and IHC(P) applications, as indicated by the product citations, 3 of them in the last year!
Date published: 2017-01-31
Rated 5 out of 5 by from Works well for IF with human endothelialWorks well for IF with human endothelial cells and placenta. -SCBT Publication Review
Date published: 2015-04-07
Rated 3 out of 5 by from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-03-19
Rated 5 out of 5 by from Single clean band in HeLa WCLSingle clean band in HeLa WCL. -SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by from Robust staining in paraffinRobust staining in paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-02-12
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Cytokeratin 16 抗体 (LL025) is rated 4.6 out of 5 by 5.
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