Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Cytokeratin 15 Antibody (LHK15) - Western Blotting - Image 14543
细胞角蛋白 15 抗体 (LHK15): sc-47697. 对 A-431 全细胞裂解液中细胞角蛋白 15 的表达进行 Western 印迹分析.
细胞角蛋白 15 抗体 (LHK15): sc-47697. 对 A-431 全细胞裂解液中细胞角蛋白 15 的表达进行 Western 印迹分析.

Cytokeratin 15 抗体 (LHK15): sc-47697

4.6(8)
寫評論提問

说明书
  • Cytokeratin 15 抗体 LHK15 是小鼠单克隆 IgG2a κ，Cytokeratin 15抗体, 在46篇文献中引用，规格为200 µg/ml
  • 针对human起源的Cytokeratin 15的最后17个氨基酸
  • Cytokeratin 15 抗体 (LHK15) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Cytokeratin 15
  • 抗Cytokeratin 15抗体（LHK15）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 15 (LHK15): sc-47697的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Cytokeratin 15 抗体 (LHK15) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cytokeratin 15 抗体 (LHK15) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cytokeratin 抗体 包括 Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17和Cytokeratin 18

细胞角蛋白15抗体（LHK15）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的细胞角蛋白15蛋白。抗细胞角蛋白15抗体（LHK15）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。细胞角蛋白15（CK15、K15、K1CO、角蛋白15、I型细胞骨架15）是一种中间丝蛋白，在维持上皮细胞（尤其是毛囊）的机械完整性方面发挥着至关重要的作用。细胞角蛋白15主要位于毛囊隆起处，是干细胞的特定标记，这凸显了细胞角蛋白15在毛囊生物学和再生中的重要性。细胞角蛋白15的表达对于区分基底细胞癌和毛上皮瘤非常重要，因为这两种疾病具有重叠的形态特征，可能会导致诊断复杂化。细胞角蛋白15和细胞角蛋白19的表达模式，以及毛发角蛋白的缺失，为这些肿瘤的分化状态提供了宝贵的见解，使细胞角蛋白15单克隆抗体（LHK15）成为皮肤病理学研究人员和临床医生必不可少的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 15 抗体 (LHK15) 参考文献:

  1. 角蛋白 15 在分层上皮中的表达：在活化的角质形成细胞中下调。  |  Waseem, A., et al. 1999. J Invest Dermatol. 112: 362-9. PMID: 10084315
  2. 皮肤乳头状肿瘤中毛干细胞特异性角蛋白 15 的表达。  |  Kanitakis, J., et al. 1999. Eur J Dermatol. 9: 363-5. PMID: 10417438
  3. 体外转化生长因子 beta 和体内皮肤损伤抑制角蛋白 15 的表达。  |  Werner, S. and Munz, B. 2000. Exp Cell Res. 254: 80-90. PMID: 10623468
  4. 人类角蛋白 15 和角蛋白 19 基因的基因组组织和扩增。  |  Whittock, NV., et al. 2000. Biochem Biophys Res Commun. 267: 462-5. PMID: 10623642
  5. 人乳腺上皮细胞系中角蛋白 15 和角蛋白 17 的凋亡诱导裂解。  |  Badock, V., et al. 2001. Cell Death Differ. 8: 308-15. PMID: 11319614
  6. 作为滤泡分化标志物的细胞角蛋白：对毛母细胞瘤和基底细胞癌的免疫组化研究。  |  Kurzen, H., et al. 2001. Am J Dermatopathol. 23: 501-9. PMID: 11801790
  7. 角蛋白 15 启动子靶向毛囊隆起区的假定上皮干细胞。  |  Liu, Y., et al. 2003. J Invest Dermatol. 121: 963-8. PMID: 14708593
  8. 原位和侵袭性皮肤上皮病变中的干细胞标记物（细胞角蛋白15、细胞角蛋白19和p63）。  |  Abbas, O., et al. 2011. Mod Pathol. 24: 90-7. PMID: 20852595
  9. 细胞角蛋白7、细胞角蛋白15、细胞角蛋白19、CAM 5.2、癌胚抗原和巢蛋白在区分腺癌和鳞状细胞癌中的免疫组织化学比较。  |  Mahalingam, M., et al. 2012. Hum Pathol. 43: 1265-72. PMID: 22285043
  10. 基底细胞癌中细胞角蛋白15、细胞角蛋白19、卵泡抑制素和Bmi-1的免疫组织化学表达。  |  Kim, HC., et al. 2016. Int J Dermatol. 55: 36-44. PMID: 26739632
  11. 细胞角蛋白 15 是食管鳞状细胞癌进展和恶性程度的有效指标  |  Shen, YH., et al. 2016. Asian Pac J Cancer Prev. 17: 4217-4222. PMID: 27797221
  12. 瘢痕性脱发患者细胞角蛋白-15缺失缺乏特异性。  |  Mahalingam, M. 2017. J Am Acad Dermatol. 76: e135-e136. PMID: 28325418

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 15 抗体 (LHK15)

sc-47697
200 µg/ml
RMB2377.00

Cytokeratin 15 (LHK15): m-IgG Fc BP-HRP 套装

sc-525552
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 15 (LHK15): m-IgG2a BP-HRP 套装

sc-546311
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 15 抗体 (LHK15) AC

sc-47697 AC
500 µg/ml, 25% agarose
RMB3129.00

Cytokeratin 15 抗体 (LHK15) HRP

sc-47697 HRP
200 µg/ml
RMB2377.00

Cytokeratin 15 抗体 (LHK15) FITC

sc-47697 FITC
200 µg/ml
RMB2482.00

Cytokeratin 15 抗体 (LHK15) PE

sc-47697 PE
200 µg/ml
RMB2580.00

Cytokeratin 15 抗体 (LHK15) Alexa Fluor® 488

sc-47697 AF488
200 µg/ml
RMB2685.00

Cytokeratin 15 抗体 (LHK15) Alexa Fluor® 546

sc-47697 AF546
200 µg/ml
RMB2685.00

Cytokeratin 15 抗体 (LHK15) Alexa Fluor® 594

sc-47697 AF594
200 µg/ml
RMB2685.00

Cytokeratin 15 抗体 (LHK15) Alexa Fluor® 647

sc-47697 AF647
200 µg/ml
RMB2685.00

Cytokeratin 15 抗体 (LHK15) Alexa Fluor® 680

sc-47697 AF680
200 µg/ml
RMB2685.00

Cytokeratin 15 抗体 (LHK15) Alexa Fluor® 790

sc-47697 AF790
200 µg/ml
RMB2685.00

Pricing up to buy this:  200ug/ml,  but what volume?

Asked by: Shelly99
Thank you for your question. This is provided as 200 ug in 1 ml volume.
Answered by: Technical Support
Date published: 2020-03-25

Dear SAnta cruz,I wanted to try cytokeratin 15 antibody. But since its mouse antibody i am not sure if it will give me lot of background with my mouse skin sample.Is their any possibility to get free small aliquote for trail Please

Asked by: zumer
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Can Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2017-01-10
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_47697, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 96ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from super clean stain on skin!looks great by IF on a FFPE human skin biopsy, very clean stain
Date published: 2024-01-10
Rated 5 out of 5 by from works very well in histology alone or in coIFSerial sections from human scalp biopsies and mouse ears embedded in OCT or human scalp biopsies embedded in formalin‐fixed paraffin were processed for immunofluorescence as follow: Frozen sections were fixed with neutral buffered 4% paraformaldehyde (PFA) for 15 min at room temperature (RT) and washed three times with phosphate‐buffered saline (PBS). Paraffin sections were processed for their deparaffinization and antigen retrieval. Primary antibody specific for Keratin 15 at 1:400 dilution was incubated overnight (O/N) at 4°C. Sections were then washed three times with PBS and incubated with secondary antibody conjugated to the appropriate fluorophore(s) (for example, Alexa Fluor 647 dilution 1:200) for 1 h at RT. Sections were then washed three times with PBS, counterstained with DAPI, mounted with IF-grate antifade mounting medium and analyzed by fluorescence or confocal microscopy.
Date published: 2019-12-04
Rated 4 out of 5 by from good antibodythe antibody was tested for flow cytometry in mammary cells. worked well.
Date published: 2017-11-23
Rated 5 out of 5 by from Outstanding IHC stainingGreat product for IHC staining applications; also nice WB
Date published: 2017-01-14
Rated 5 out of 5 by from Great IHC(P) dataThe Cytokeratin 15 Antibody (LHK15): sc-47697 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has been cited over 10 times for further verification.
Date published: 2017-01-10
Rated 5 out of 5 by from Excellent images obtained in IF with humanExcellent images obtained in IF with human placenta -SCBT Publication Review
Date published: 2015-03-07
Rated 5 out of 5 by from Works well in IHC with paraffinWorks well in IHC with paraffin-embedded human fetal corneal cells -SCBT Publication Review
Date published: 2015-03-06
Rated 3 out of 5 by from Good single band with some backgroundGood single band with some background -SCBT QC
Date published: 2015-02-15
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_47697, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
Cytokeratin 15 抗体 (LHK15) is rated 4.6 out of 5 by 8.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_47697, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 139ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。