Date published: 2025-9-6
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Cytokeratin 1 Antibody (4D12B3) - Western Blotting - Image 367493
细胞角蛋白 1 抗体 (4D12B3): sc-65999. 细胞角蛋白 1 在 HeLa (A), SW480 (B) 和 A-431 (C) 全细胞裂解液及小鼠胃组织提取物 (D) 中表达的 Western 印迹分析.
细胞角蛋白 1 抗体 (4D12B3): sc-65999. 细胞角蛋白 1 在 HeLa (A), SW480 (B) 和 A-431 (C) 全细胞裂解液及小鼠胃组织提取物 (D) 中表达的 Western 印迹分析.

Cytokeratin 1 抗体 (4D12B3): sc-65999

3.7(6)
寫評論提問

说明书
  • Cytokeratin 1抗体(4D12B3)是小鼠单克隆IgG1 κ, 在15篇文献中引用，规格为200 µg/ml
  • 针对纯化的截短重组human的Cytokeratin 1
  • 抗-Cytokeratin 1 抗体 (4D12B3) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Cytokeratin 1
  • 抗Cytokeratin 1抗体（4D12B3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 1 (4D12B3): sc-65999的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Cytokeratin 1 抗体 (4D12B3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cytokeratin 1 抗体 (4D12B3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cytokeratin 抗体 包括 Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17和Cytokeratin 18

细胞角蛋白 1 抗体 (4D12B3) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和免疫组织化学等应用检测小鼠、大鼠和人体样本中的细胞角蛋白 1。抗细胞角蛋白 1 抗体 (4D12B3) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。细胞角蛋白 1 是细胞角蛋白家族的重要成员，由对维持上皮细胞结构完整性至关重要的中间丝蛋白组成。这些蛋白主要位于角质形成细胞的细胞质中，形成一个网络，在压力下为细胞提供机械支持和弹性。细胞角蛋白 1 在表皮中起着至关重要的作用，是角质形成细胞细胞骨架的重要组成部分，有助于皮肤屏障功能的形成。细胞角蛋白 1 是组织分化的关键标志物，有助于鉴定各种恶性肿瘤，包括上皮样血管内皮细胞瘤、血管肉瘤、分裂瘤、上皮样肉瘤和滑膜肉瘤。人类细胞角蛋白 1 基因位于染色体 12q13.13，该基因突变可导致丝状关联异常，从而导致表皮溶解性角化过度症等疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 1 抗体 (4D12B3)

sc-65999
200 µg/ml
RMB2377.00

Cytokeratin 1 (4D12B3): m-IgG Fc BP-HRP 套装

sc-526971
200 µg Ab; 10 µg BP
RMB2662.00

Cytokeratin 1 (4D12B3): m-IgG1 BP-HRP 套装

sc-532344
200 µg Ab; 20 µg BP
RMB2662.00

Cytokeratin 1 抗体 (4D12B3) AC

sc-65999 AC
500 µg/ml, 25% agarose
RMB3129.00

Cytokeratin 1 抗体 (4D12B3) HRP

sc-65999 HRP
200 µg/ml
RMB2377.00

Cytokeratin 1 抗体 (4D12B3) FITC

sc-65999 FITC
200 µg/ml
RMB2482.00

Cytokeratin 1 抗体 (4D12B3) PE

sc-65999 PE
200 µg/ml
RMB2580.00

Cytokeratin 1 抗体 (4D12B3) Alexa Fluor® 488

sc-65999 AF488
200 µg/ml
RMB2685.00

Cytokeratin 1 抗体 (4D12B3) Alexa Fluor® 546

sc-65999 AF546
200 µg/ml
RMB2685.00

Cytokeratin 1 抗体 (4D12B3) Alexa Fluor® 594

sc-65999 AF594
200 µg/ml
RMB2685.00

Cytokeratin 1 抗体 (4D12B3) Alexa Fluor® 647

sc-65999 AF647
200 µg/ml
RMB2685.00

Cytokeratin 1 抗体 (4D12B3) Alexa Fluor® 680

sc-65999 AF680
200 µg/ml
RMB2685.00

Cytokeratin 1 抗体 (4D12B3) Alexa Fluor® 790

sc-65999 AF790
200 µg/ml
RMB2685.00

Mab 4D12B3 anti-cytokeratin antibody is reared to "...a truncated portion of cytokeratin 1..." What is this epitope? Does it include the following sequence" G143VPCPPGGIQEVTINQSLLQ162? Please advise.

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-11-10

I do not see the answer to my question. What is the epitope region of the following Mab to CK1? Mab 4D12B3 anti-cytokeratin antibody is reared to "...a truncated portion of cytokeratin 1..." What is this epitope? Does it include the following sequence"

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-11-10

Can Cytokeratin 1 (4D12B3): sc-65999 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, Cytokeratin 1 (4D12B3): sc-65999 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Great, Versatile AntibodyThis antibody worked great for our lab on FFPE canine tissues using a variety of conditions. Retrieval worked equally well using both EDTA pH9 and citrate pH6 buffers, and signal was still quite strong at a 1:500 dilution, though somewhere between 1:250 and 1:500 would probably be optimal.
Date published: 2021-02-10
Rated 1 out of 5 by from Didn't work well for IHCThis antibody didn't work well on formalin fixed, paraffin-embedded mouse skin tissue.
Date published: 2017-08-10
Rated 4 out of 5 by from ParaffinParaffin-embedded IHC publication using human keratinocytes. -SCBT Publication Review
Date published: 2015-03-18
Rated 4 out of 5 by from WB publication using HaCaT cell line (keratinocytes)WB publication using HaCaT cell line (keratinocytes) -SCBT Publication Review
Date published: 2015-03-13
Rated 4 out of 5 by from Signal via immunoperoxidase staining of formalinSignal via immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing cytoplasmic staining of squamous epithelial cells. -SCBT QC
Date published: 2015-02-22
Rated 4 out of 5 by from Positive western blot analysis of truncatedPositive western blot analysis of truncated human recombinant Cytokeratin 1 protein. -SCBT QC
Date published: 2015-01-29
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Cytokeratin 1 抗体 (4D12B3) is rated 3.7 out of 5 by 6.
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