Date published: 2025-9-7
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CyPA Antibody (6-YD13) - Western Blotting - Image 368002
CyPA 抗体 (6-YD13): sc-134310. Jurkat (A), A-431 (B) 和 HeLa (C) 全细胞裂解液中 CyPA 表达的 Western 印迹分析.
CyPA 抗体 (6-YD13): sc-134310. Jurkat (A), A-431 (B) 和 HeLa (C) 全细胞裂解液中 CyPA 表达的 Western 印迹分析.

CyPA 抗体 (6-YD13): sc-134310

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寫評論提問

说明书
  • CyPA 抗体 6-YD13 是小鼠单克隆 IgG2a κ，CyPA抗体, 在11篇文献中引用，规格为100 µg/ml
  • 针对全长CyPA，human起源
  • 推荐用于 mouse, rat 和 human 来源的CyPA WB, IP 和 ELISA检测
  • 关于如何获取CyPA (6-YD13): sc-134310的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是CyPA Antibody (6-YD13) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CyPA Antibody (6-YD13) 打包提供（请参阅下面的订购信息）。

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関連項目

额外Cyclophilin抗体，包括Cyclophilin E, Cyclophilin F, Cyclophilin G, CyPA, CyPB和Cyclophilin 40

CyPA Antibody (6-YD13)是一种IgG2a κ小鼠单克隆CyPA抗体(也称为PPIA抗体、肽基脯氨酰顺反异构酶A抗体、环孢素A结合蛋白抗体、亲环素A抗体、轮状酶A抗体、PPIase A抗体、肽基脯氨酰异构酶A (Cyclophilin A)抗体、T细胞亲环素抗体或CYPH抗体)，通过WB、IP和ELISA检测小鼠、大鼠和人源的CyPA蛋白。CyPA抗体(6-YD13)是一种非偶联的抗CyPA抗体。免疫抑制剂环孢素A (CsA)与亲环素和钙调神经磷酸酶形成三分子复合物来抑制钙调神经磷酸酶活性。亲环素是一类广泛存在于胞质内的肽基-脯氨酰顺反异构酶，能够促进XaaPro肽键的异构化和蛋白质的复性。人亲环素A (CyPA)是一种由165个氨基酸组成的细胞内蛋白，是CsA的靶点，由从酵母到人的一个独特基因编码。CyPA以参与T细胞分化和增殖而闻名，并在脑中高表达。CyPA通过与病毒Gag多聚蛋白的衣壳结构域直接相互作用而被整合到1型人类免疫缺陷病毒(HIV-1)的病毒粒子中，对病毒的高效复制至关重要。亲环素B (Cyclophilin B, CyPB)是亲环素家族的一员，具有特异性的N端和c端延伸。与CyPA不同，CyPB有一个信号序列导致其在内质网易位。CyPB在血液或乳汁等生物液体中分泌，并与人淋巴母细胞细胞系Jurkat和人外周血淋巴细胞上的特异性受体结合(9,10)。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CyPA 抗体 (6-YD13) 参考文献:

  1. HIV-1帽状蛋白的成熟诱导构象变化以及在C-末端结构域鉴定出两个与环纤蛋白的高亲和力位点。  |  Endrich, MM., et al. 1999. J Biol Chem. 274: 5326-32. PMID: 10026140
  2. 对新生隐球菌的生长和毒力具有重要作用的两种环嗜蛋白 A 同源物，其功能既有相同之处，也有不同之处。  |  Wang, P., et al. 2001. EMBO Rep. 2: 511-8. PMID: 11415984
  3. 环嗜蛋白 A 表达改变对人类和小鼠神经细胞生长和分化的影响  |  Nahreini, P., et al. 2001. Cell Mol Neurobiol. 21: 65-79. PMID: 11440199
  4. s-cyclophilin 通过独特的 COOH 端序列保留在细胞内，并与钙储存蛋白 calreticulin 共同定位。  |  Arber, S., et al. 1992. J Cell Biol. 116: 113-25. PMID: 1530944
  5. 一种内质网特异性环纤蛋白。  |  Hasel, KW., et al. 1991. Mol Cell Biol. 11: 3484-91. PMID: 1710767
  6. 原位检测环孢素 A：环孢素和环纤蛋白核定位的证据。  |  Le Hir, M., et al. 1995. Lab Invest. 73: 727-33. PMID: 7474947
  7. 十聚体环孢素-环孢菌素晶体复合物的 X 射线结构。  |  Pflügl, G., et al. 1993. Nature. 361: 91-4. PMID: 8421501
  8. 有证据表明，母乳中分离出的环纤蛋白 B 是一种截短形式。  |  Mariller, C., et al. 1996. Biochim Biophys Acta. 1293: 31-8. PMID: 8652625
  9. 环芬蛋白 B 的 N 端参与了与特定 Jurkat T 细胞结合点的相互作用。  |  Mariller, C., et al. 1996. Biochem J. 317 (Pt 2): 571-6. PMID: 8713087
  10. cyclophilin A 与 HIV-1 帽状蛋白结合部位肽复合物的晶体结构。  |  Vajdos, FF., et al. 1997. Protein Sci. 6: 2297-307. PMID: 9385632

订购信息

产品名称产品编号规格价格数量收藏夹

CyPA 抗体 (6-YD13)

sc-134310
100 µg/ml
RMB2504.00

CyPA (6-YD13): m-IgG Fc BP-HRP 套装

sc-537365
100 µg Ab; 10 µg BP
RMB2662.00

CyPA (6-YD13): m-IgGκ BP-HRP 套装

sc-534612
100 µg Ab; 40 µg BP
RMB2662.00

CyPA (6-YD13): m-IgG2a BP-HRP 套装

sc-547993
100 µg Ab; 10 µg BP
RMB2662.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
Thank you for your question We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 1 out of 5 by from Not nearly as good as their old rabbit polyclonal!I did an IP to pull down CypA (using abcam's antibody as its better), and then came back with this 6-YD13 mouse monoclonal antibody (right hand blot on image). This is in HEK cells. The antibody band for CypA is very weak signal and the antibody bands for the light chain and heavy chain from the IP beads is very overpowering- I never saw this with the old CypA antibody. I thought that maybe the IP extract is degraded (unlikely though as I've done this lots of times before using my protocol). So I reprobed the same blot with the old C-14 polyclonal rabbit antibody I had left over and the bands for CypA are clear as day . Both antibodies were used at 1:1000 diluted in 5% BSA PBST. I can't increase the amount of 6-YD13 used as it will also increase the signal of the light/heavy chain and will bleach the CypA signal more.
Date published: 2017-07-17
Rated 5 out of 5 by from Strong signal in WBVery clear band in WB at 1:500 dilution. Several publications available for WB and IF in mouse and human samples.
Date published: 2016-12-21
Rated 5 out of 5 by from powerful toolMade outstanding contribution in my work and paper。
Date published: 2016-11-03
Rated 5 out of 5 by from Robust band in western blot analysis of CyPARobust band in western blot analysis of CyPA expression in K-562 whole cell lysate. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Strong band present in western blot usingStrong band present in western blot using Jurkat whole cell lysate. -SCBT QC
Date published: 2013-10-04
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CyPA 抗体 (6-YD13) is rated 4.2 out of 5 by 5.
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