Date published: 2025-9-7

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cyclin T1 抗体 (E-3): sc-271348

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说明书
  • cyclin T1 抗体 E-3 是小鼠单克隆 IgG2b κ,cyclin T1抗体, 在23篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的cyclin T1的C-terminus附近的氨基酸671-708之间
  • cyclin T1 抗体 (E-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 cyclin T1
  • 抗cyclin T1抗体(E-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-271348 X, 200 µg/0.1 ml)
  • 关于如何获取cyclin T1 (E-3): sc-271348免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是cyclin T1 Antibody (E-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与cyclin T1 Antibody (E-3) 打包提供(请参阅下面的订购信息)。

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関連項目

cyclin T1抗体(E-3)是一种IgG2b κ小鼠单克隆cyclin T1抗体(也称为Cyclin-T抗体或CCNT1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人类的cyclin T1蛋白。cyclin T1抗体(E-3)既可作为非共轭抗cyclin T1抗体形式,也可作为多种共轭形式的抗cyclin T1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Cyclin T1被鉴定为Cdk9的伴侣,Cdk9是RNA聚合酶II(RNAPII)的转录延伸因子。Cyclin T1与HIV-1 Tat蛋白的反式激活域相互作用。Tat与cyclin T1的相互作用增强了Tat对病毒TAR RNA茎环结构的亲和力,这表明Tat可以通过与TAR的协同结合来招募cyclin T1/cdk9至RNAPII。人类阳性转录延伸因子b(P-TEFb)由细胞周期蛋白依赖性激酶cdk9和细胞周期蛋白T组成。Cdk9可以以相互排斥的方式与cyclin T1或cyclin T2配对。cyclin T2的两种形式T2a和T2b是由于选择性剪接而产生的。人类cyclin T1可以促进Tat与TAR的结合,但cyclins T2a或T2b却不能。cyclin T2与Cdk9结合,但不与Tat结合,且cyclin T2可以抑制cyclin T1介导的Tat活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin T1 抗体 (E-3) 参考文献:

  1. 人类细胞周期蛋白 T1 可促进 Tat 和 TAR 之间的相互作用以及人类免疫缺陷病毒的复制,但细胞周期蛋白 T2a 或 T2b 却不能。  |  Wimmer, J., et al. 1999. Virology. 255: 182-9. PMID: 10049833
  2. 与 CDK9 相关的细胞周期蛋白 T1 和 T2 对 HIV-1 Tat 的活性具有相反的作用。  |  Napolitano, G., et al. 1999. AIDS. 13: 1453-9. PMID: 10465067
  3. 前列素对细胞周期蛋白 T1/P-TEFb 功能和原代静息 CD4+ T 细胞基因表达谱的影响  |  Sung, TL. and Rice, AP. 2006. Retrovirology. 3: 66. PMID: 17014716
  4. PRMT6 对人类免疫缺陷病毒 1 型 Tat 蛋白的精氨酸甲基化会对 Tat 与细胞周期蛋白 T1 和 Tat 转录激活区的相互作用产生负面影响。  |  Xie, B., et al. 2007. J Virol. 81: 4226-34. PMID: 17267505
  5. 含有 I-mfa 结构域的发育调节因子与 T 细胞周期蛋白和 Tat 相互作用并调节转录。  |  Wang, Q., et al. 2007. J Mol Biol. 367: 630-46. PMID: 17289077
  6. 将抑制 CDK9-Cyclin T1 蛋白-蛋白相互作用作为抗击三阴性乳腺癌的新方法。  |  Cheng, SS., et al. 2022. Acta Pharm Sin B. 12: 1390-1405. PMID: 35530158
  7. 发现CDK9-Cyclin T1复合物的小分子降解剂,用于靶向前列腺癌的转录成瘾。  |  Li, J., et al. 2022. J Med Chem. 65: 11034-11057. PMID: 35925880
  8. 发现基于HyT的CDK9-Cyclin T1复合物降解剂。  |  Lin, R., et al. 2023. Chem Biodivers. 20: e202300769. PMID: 37349855
  9. 慢病毒 Tat 蛋白与细胞蛋白激酶 TAK 特异性结合,TAK 可使 RNA 聚合酶 II 大亚基的羧基末端结构域过度磷酸化:Tat 辅因子的候选者。  |  Herrmann, CH. and Rice, AP. 1995. J Virol. 69: 1612-20. PMID: 7853496
  10. TAK是一种与HIV Tat相关的激酶,属于细胞周期蛋白依赖性蛋白激酶家族的成员,外周血淋巴细胞的活化和原核细胞系的分化都会诱导TAK。  |  Yang, X., et al. 1997. Proc Natl Acad Sci U S A. 94: 12331-6. PMID: 9356449
  11. 一种新型 CDK9 相关 C 型细胞周期蛋白与 HIV-1 Tat 直接相互作用,并介导其与 TAR RNA 的高亲和力, 环特异性结合。  |  Wei, P., et al. 1998. Cell. 92: 451-62. PMID: 9491887
  12. 鉴定人类 P-TEFb 的多个细胞周期蛋白亚基。  |  Peng, J., et al. 1998. Genes Dev. 12: 755-62. PMID: 9499409

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin T1 抗体 (E-3)

sc-271348
200 µg/ml
RMB2377.00

cyclin T1 (E-3): m-IgGκ BP-HRP 套装

sc-521918
200 µg Ab, 40 µg BP
RMB2662.00

cyclin T1 (E-3): m-IgG2b BP-HRP 套装

sc-548985
200 µg Ab; 10 µg BP
RMB2662.00

cyclin T1 抗体 (E-3) X

sc-271348 X
200 µg/0.1 ml
RMB2377.00

cyclin T1 抗体 (E-3) AC

sc-271348 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin T1 抗体 (E-3) HRP

sc-271348 HRP
200 µg/ml
RMB2377.00

cyclin T1 抗体 (E-3) FITC

sc-271348 FITC
200 µg/ml
RMB2482.00

cyclin T1 抗体 (E-3) PE

sc-271348 PE
200 µg/ml
RMB2580.00

cyclin T1 抗体 (E-3) Alexa Fluor® 488

sc-271348 AF488
200 µg/ml
RMB2685.00

cyclin T1 抗体 (E-3) Alexa Fluor® 546

sc-271348 AF546
200 µg/ml
RMB2685.00

cyclin T1 抗体 (E-3) Alexa Fluor® 594

sc-271348 AF594
200 µg/ml
RMB2685.00

cyclin T1 抗体 (E-3) Alexa Fluor® 647

sc-271348 AF647
200 µg/ml
RMB2685.00

cyclin T1 抗体 (E-3) Alexa Fluor® 680

sc-271348 AF680
200 µg/ml
RMB2685.00

cyclin T1 抗体 (E-3) Alexa Fluor® 790

sc-271348 AF790
200 µg/ml
RMB2685.00

cyclin T1 (E-3) 中和勝肽

sc-271348 P
100 µg/0.5 ml
RMB511.00

I am looking for cyclin T1 antibody for IF assay. Host must be mouse. which one do you recommend?

Asked by: lilyk
Thank you for your question. Here is a link for a recommended antibody : https://www.scbt.com/p/cyclin-t1-antibody-c-6
Answered by: BlakeJ
Date published: 2023-07-11

What application is the blocking peptide sc-271348 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good productIt is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 4 out of 5 by from Single band in western blotUsed at 1:500 to detect endogenous protein in 30ug nuclear extract from HEK293T.
Date published: 2018-08-02
Rated 5 out of 5 by from Great for WBThis antibody is good for Western blot with human cell nuclear extract.
Date published: 2018-01-13
Rated 5 out of 5 by from Great for western blot and work for IP as wellGreat for western blot and work for IP as well. We used it for MCF-7 cell lysate.
Date published: 2017-11-30
Rated 5 out of 5 by from a fantastic antibody for WBThis antibody generates a clear and very strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from clean bandThis antibody works well in western bloting experiments.The band is clean although the antibody titer is not high enough.
Date published: 2017-08-18
Rated 5 out of 5 by from Beautiful signalthis antibody works very well for western blotting; really clean Cyclin T1 band; I used it on protein extracts from different types of human cells (like HeLa, HCT116,etc). looks great.
Date published: 2017-04-21
Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, strong band detected in K-562 and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-01-09
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cyclin T1抗体 (E-3) is rated 4.9 out of 5 by 8.
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