Date published: 2025-9-7

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cyclin D3 抗体 (D-7): sc-6283

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说明书
  • cyclin D3 抗体 D-7 是小鼠单克隆 IgG1 κ,cyclin D3抗体, 在97篇文献中引用,规格为200 µg/ml
  • 免疫1-292氨基酸,对应于human来源cyclin D3全长
  • cyclin D3 抗体 (D-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 cyclin D3
  • 抗cyclin D3抗体(D-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取cyclin D3 (D-7): sc-6283免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 cyclin D3 抗体 (D-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cyclin D3 抗体 (D-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Cyclin D3抗体(D-7)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和酶联免疫吸附等方法检测小鼠、大鼠和人源的Cyclin D3。Cyclin D3 (D-7) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cyclin D3 在细胞周期调节中起着关键作用,特别是在 G1 期,cyclin D3 对于向 S 期过渡至关重要。这种转变对于DNA复制和细胞分裂至关重要,因此细胞周期蛋白D3在细胞增殖和生长中扮演着关键角色。细胞周期蛋白D3失调与多种癌症有关,因为细胞周期蛋白D3会导致细胞分裂失控。细胞周期蛋白D3与细胞周期蛋白依赖性激酶(CDK)相互作用,促进靶蛋白的磷酸化,这是细胞周期进展所必需的。了解细胞周期蛋白D3的功能和调节机制对于制定治疗异常细胞周期控制癌症的策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin D3 抗体 (D-7) 参考文献:

  1. 从鼠红细胞白血病细胞中克隆出一种 D 型细胞周期蛋白。  |  Kiyokawa, H., et al. 1992. Proc Natl Acad Sci U S A. 89: 2444-7. PMID: 1372445
  2. 克隆和鉴定人类细胞周期蛋白 D3,这是一种与 PRAD1/细胞周期蛋白 D1 原癌基因序列密切相关的 cDNA。  |  Motokura, T., et al. 1992. J Biol Chem. 267: 20412-5. PMID: 1383201
  3. 人类细胞周期蛋白 D 基因的基因组结构, 染色体定位和独立表达。  |  Inaba, T., et al. 1992. Genomics. 13: 565-74. PMID: 1386335
  4. 编码人类 D 型细胞周期蛋白的 CCND 基因的分子克隆和染色体图谱。  |  Xiong, Y., et al. 1992. Genomics. 13: 575-84. PMID: 1386336
  5. 人二倍体成纤维细胞中 D 型细胞周期蛋白基因的生长调控表达。  |  Won, KA., et al. 1992. Proc Natl Acad Sci U S A. 89: 9910-4. PMID: 1409718
  6. 人类 D 型细胞周期蛋白  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
  7. 真核生物的细胞周期控制:cdc2 激活的分子机制。  |  Draetta, G. 1990. Trends Biochem Sci. 15: 378-83. PMID: 2147521
  8. 在ErbB2诱导的乳腺肿瘤发生和进展过程中,细胞周期蛋白-D3可补偿细胞周期蛋白-D1的缺失。  |  Zhang, Q., et al. 2011. Cancer Res. 71: 7513-24. PMID: 22037875
  9. 人胰岛细胞中细胞周期蛋白-D1和细胞周期蛋白-D3的亚细胞分布因胰腺捐赠者的状况而异。  |  Taniguchi, K., et al. 2015. Diabetologia. 58: 2056-63. PMID: 26055066
  10. 细胞周期蛋白-D3缺乏抑制皮肤肿瘤的发展,但不影响正常的角质形成细胞增殖。  |  Lee, SH., et al. 2017. Oncol Lett. 14: 2723-2734. PMID: 28927034
  11. 视网膜母细胞瘤蛋白与哺乳动物 D 型细胞周期蛋白的功能相互作用  |  Ewen, ME., et al. 1993. Cell. 73: 487-97. PMID: 8343202
  12. 视网膜母细胞瘤蛋白与人类 D 细胞周期蛋白的物理相互作用。  |  Dowdy, SF., et al. 1993. Cell. 73: 499-511. PMID: 8490963

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin D3 抗体 (D-7)

sc-6283
200 µg/ml
RMB2377.00

cyclin D3 (D-7): m-IgG Fc BP-HRP 套装

sc-528167
200 µg Ab; 10 µg BP
RMB2662.00

cyclin D3 (D-7): m-IgGκ BP-HRP 套装

sc-520476
200 µg Ab, 40 µg BP
RMB2662.00

cyclin D3 抗体 (D-7) AC

sc-6283 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin D3 抗体 (D-7) HRP

sc-6283 HRP
200 µg/ml
RMB2377.00

cyclin D3 抗体 (D-7) FITC

sc-6283 FITC
200 µg/ml
RMB2482.00

cyclin D3 抗体 (D-7) PE

sc-6283 PE
200 µg/ml
RMB2580.00

cyclin D3 抗体 (D-7) Alexa Fluor® 488

sc-6283 AF488
200 µg/ml
RMB2685.00

cyclin D3 抗体 (D-7) Alexa Fluor® 546

sc-6283 AF546
200 µg/ml
RMB2685.00

cyclin D3 抗体 (D-7) Alexa Fluor® 594

sc-6283 AF594
200 µg/ml
RMB2685.00

cyclin D3 抗体 (D-7) Alexa Fluor® 647

sc-6283 AF647
200 µg/ml
RMB2685.00

cyclin D3 抗体 (D-7) Alexa Fluor® 680

sc-6283 AF680
200 µg/ml
RMB2685.00

cyclin D3 抗体 (D-7) Alexa Fluor® 790

sc-6283 AF790
200 µg/ml
RMB2685.00

cyclin D3 抗体 (D-7) TRITC

sc-6283 TRITC
200 µg/ml
RMB2407.00

Can this mouse monoclonal antibody be used for immunohistochemical staining of mouse tissue?

Asked by: AbPolly
Thank you for your question. Yes, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is available conjugated to HRP, PE, FITC, TRITC, AF488 and AF647.
Answered by: Technical Support 9
Date published: 2017-01-20
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Rated 1 out of 5 by from Non-specific Western detection in rat INS-1 lysateTested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but only non-specific bands were detected. Recommend using sc-56307
Date published: 2019-05-22
Rated 4 out of 5 by from Worked well for WBworked well for WB using human tissue lysates at 1:200
Date published: 2018-03-22
Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 4 out of 5 by from Faint but distinct bands on WBFaint but distinct bands on WB. Used the antibody at 1:1000 dilution.
Date published: 2017-07-03
Rated 3 out of 5 by from not suitable for turbotthere was no band in turbot, primary antibody 1:200, second antibody 1:2000. so i dont think it is suitable for turbot
Date published: 2017-06-20
Rated 5 out of 5 by from suitable for WBWe use this antibody routinely by WB, it always gives very good signal,1:400 dilution .
Date published: 2017-06-03
Rated 5 out of 5 by from Good WB analysisWe detected positive signal in WB using Hela cell,I highly recommend use 1:600 diluiton
Date published: 2017-05-28
Rated 3 out of 5 by from WBWe have got many none specific bands in human macrophage lysate.
Date published: 2017-04-23
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cyclin D3 抗体 (D-7) is rated 4.3 out of 5 by 17.
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