Date published: 2025-9-7

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cyclin D2 抗体 (34B1-3): sc-452

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说明书
  • cyclin D2 抗体 34B1-3 是大鼠单克隆 IgG2a, 在85篇文献中引用,提供 200 µg/ml
  • 针对重组cyclin D2蛋白的抗原性氨基酸
  • 抗-cyclin D2 抗体 (34B1-3) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 cyclin D2 and, to a lesser extent, cyclin D1; 不会与 cyclin D3产生交联反应
  • 抗cyclin D2抗体(34B1-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取cyclin D2 (34B1-3): sc-452免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成cyclin D2 抗体 (34B1-3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Cyclin D2抗体(34B1-3)是一种鼠单克隆IgG2a抗体,用于检测细胞周期调节中起关键作用的蛋白cyclin D2。Cyclin D2,也称为Ccnd2、cD2、Vin1和Vin-1,通过与细胞周期蛋白依赖性激酶(Cdk4和Cdk6)形成复合物,在控制从G1期到S期的过渡中起着至关重要的作用,随后,细胞周期蛋白依赖性激酶(Cdk4和Cdk6)磷酸化视网膜母细胞瘤(Rb)蛋白,导致激活E2F转录因子,这对于DNA合成和细胞增殖至关重要。该功能对于正常细胞生长和分裂至关重要,而细胞周期蛋白D2的失调与多种癌症有关,因此细胞周期蛋白D2成为癌症研究和治疗开发的重要靶标。Cyclin D2 (34B1-3) 单克隆抗体针对重组的 cyclin D2 蛋白,在小鼠、大鼠和人类物种中表现出强大的反应性,可用于 Western Blotting (WB)、Immunoprecipitation (IP)、Immunofluorescence (IF) 和 Immunohistochemistry (IHCP) 等应用。Cyclin D2 (34B1-3) 单克隆抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物,为各种研究应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin D2 抗体 (34B1-3) 参考文献:

  1. 人类卵巢中假基因细胞周期蛋白D2 mRNA的表达可能是卵巢功能随衰老而下降的新标志。  |  Choi, D., et al. 2001. J Assist Reprod Genet. 18: 110-3. PMID: 11285977
  2. 细胞周期蛋白D2和p27(Kip1)在细胞周期蛋白D2转基因小鼠乳腺发育过程中的功能分析。  |  Kong, G., et al. 2002. Oncogene. 21: 7214-25. PMID: 12370811
  3. 细胞周期蛋白 D2 控制着 B 细胞祖细胞的数量。  |  Mohamedali, A., et al. 2003. J Leukoc Biol. 74: 1139-43. PMID: 14612431
  4. p75NTR通过非受体机制增强PC12细胞肿瘤生长,该机制涉及细胞周期蛋白D2的下调。  |  Fritz, MD., et al. 2006. Exp Cell Res. 312: 3287-97. PMID: 16887120
  5. 细胞周期蛋白D2和细胞周期蛋白D3在小鼠皮肤癌发生中起相反的作用。  |  Rojas, P., et al. 2007. Oncogene. 26: 1723-30. PMID: 16983339
  6. 胰腺β细胞中细胞周期蛋白D2的稳定性受调控  |  He, LM., et al. 2009. Mol Endocrinol. 23: 1865-75. PMID: 19628581
  7. 细胞周期蛋白D2足以驱动β细胞的自我更新和再生。  |  Tschen, SI., et al. 2017. Cell Cycle. 16: 2183-2191. PMID: 28763258
  8. 慢性B细胞恶性肿瘤中细胞周期蛋白D2的过度表达。  |  Delmer, A., et al. 1995. Blood. 85: 2870-6. PMID: 7742549
  9. 在人类乳腺上皮细胞中,细胞周期蛋白 D2 优先于 Cdk4 激活 Cdk2。  |  Sweeney, KJ., et al. 1997. Oncogene. 14: 1329-40. PMID: 9178893
  10. 激素诱导的颗粒细胞增殖和分化:细胞周期调节因子细胞周期蛋白D2和p27Kip1的协调平衡。  |  Robker, RL. and Richards, JS. 1998. Mol Endocrinol. 12: 924-40. PMID: 9658398

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin D2 抗体 (34B1-3)

sc-452
200 µg/ml
RMB2377.00

cyclin D2 抗体 (34B1-3) AC

sc-452 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin D2 抗体 (34B1-3) HRP

sc-452 HRP
200 µg/ml
RMB2377.00

cyclin D2 抗体 (34B1-3) FITC

sc-452 FITC
200 µg/ml
RMB2482.00

cyclin D2 抗体 (34B1-3) PE

sc-452 PE
200 µg/ml
RMB2580.00

cyclin D2 抗体 (34B1-3) Alexa Fluor® 488

sc-452 AF488
200 µg/ml
RMB2685.00

cyclin D2 抗体 (34B1-3) Alexa Fluor® 546

sc-452 AF546
200 µg/ml
RMB2685.00

cyclin D2 抗体 (34B1-3) Alexa Fluor® 594

sc-452 AF594
200 µg/ml
RMB2685.00

cyclin D2 抗体 (34B1-3) Alexa Fluor® 647

sc-452 AF647
200 µg/ml
RMB2685.00

cyclin D2 抗体 (34B1-3) Alexa Fluor® 680

sc-452 AF680
200 µg/ml
RMB2685.00

cyclin D2 抗体 (34B1-3) Alexa Fluor® 790

sc-452 AF790
200 µg/ml
RMB2685.00

cyclin D2 抗体 (34B1-3) TRITC

sc-452 TRITC
200 µg/ml
RMB2407.00

Can cyclin D2 (34B1-3): sc-452 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin D2 (34B1-3): sc-452 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Detects cyclin D2Detects human cyclin D2 in transfected COS cells by immunoblot.
Date published: 2019-05-14
Rated 1 out of 5 by from Didnot work for WBDid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 4 out of 5 by from Use this antibody routinely in WB in human cellsUse this antibody routinely in WB in human cells.
Date published: 2015-07-29
Rated 4 out of 5 by from no commentno comment
Date published: 2015-04-29
Rated 5 out of 5 by from Works for WB in human A549 and mouse NIHWorks for WB in human A549 and mouse NIH 3T3 samples. -SCBT Publication Review
Date published: 2015-02-12
Rated 5 out of 5 by from Works very nicely for IF on HeLa samplesWorks very nicely for IF on HeLa samples. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Great antibody for WB and IP with human heartGreat antibody for WB and IP with human heart samples. -SCBT Publication Review
Date published: 2015-01-08
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cyclin D2 抗体 (34B1-3) is rated 4.3 out of 5 by 9.
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