Date published: 2025-9-7
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cyclin D1 Antibody (A-12) - Western Blotting - Image 350757
cyclin D1 抗体 (A-12): sc-8396. U-87 MG (A), SH-SY5Y (B), HCT-116 (C) 和 SP2/0 (D) 全细胞裂解液中细胞周期蛋白 D1 表达的 Western 印迹分析.
cyclin D1 抗体 (A-12): sc-8396. U-87 MG (A), SH-SY5Y (B), HCT-116 (C) 和 SP2/0 (D) 全细胞裂解液中细胞周期蛋白 D1 表达的 Western 印迹分析.

cyclin D1 抗体 (A-12): sc-8396

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寫評論提問

说明书
  • cyclin D1 抗体 A-12 是小鼠单克隆 IgG2b κ，cyclin D1抗体, 在732篇文献中引用，规格为200 µg/ml
  • 免疫1-295氨基酸，对应于human来源cyclin D1全长
  • cyclin D1 抗体 (A-12) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 cyclin D1; 可能与 cyclin D2产生交联反应; 不会与 cyclin D3产生交联反应
  • 抗cyclin D1抗体（A-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取cyclin D1 (A-12): sc-8396的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是cyclin D1 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与cyclin D1 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cyclin 抗体 包括 cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2和cyclin D3

Cyclin D1抗体（A-12）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类来源的Cyclin D1，包括蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学、流式细胞术（FCM）和酶联免疫吸附测定。Cyclin D1 (A-12) 抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cyclin D1 在细胞周期调节中起着关键作用，特别是在 G1 期，此时 cyclin D1 对于从 G1 期到 S 期的过渡至关重要。这种转变对于细胞增殖至关重要，因为细胞需要为DNA合成做好准备。细胞周期蛋白D1的失调与多种癌症有关，会导致细胞分裂失控。细胞周期蛋白D1结合并激活细胞周期蛋白依赖性激酶（CDK），促进视网膜母细胞瘤蛋白的磷酸化，从而释放E2F转录因子，驱动DNA复制所需基因的表达。了解细胞周期蛋白D1的功能对于阐明肿瘤发生机制和开发靶向癌症疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin D1 抗体 (A-12) 参考文献:

  1. ras活性和细胞周期蛋白D1表达：小鼠皮肤肿瘤发生的重要机制  |  Rodriguez-Puebla, ML., et al. 1999. Mol Carcinog. 24: 1-6. PMID: 10029404
  2. 乳腺癌前期和早期乳腺癌中的细胞周期蛋白 D1：对预防和治疗的影响。  |  Zhou, Q., et al. 2001. Cancer Lett. 162: 3-17. PMID: 11121857
  3. 细胞周期蛋白 D1：雄激素受体共抑制因子活性的机制和结果。  |  Petre, CE., et al. 2002. J Biol Chem. 277: 2207-15. PMID: 11714699
  4. 细胞周期控制和癌症中的Cyclin A。  |  Yam, CH., et al. 2002. Cell Mol Life Sci. 59: 1317-26. PMID: 12363035
  5. Cyclin D1抑制过氧化物酶体增殖激活受体γ的表达和转录。  |  Wang, C., et al. 2003. Mol Cell Biol. 23: 6159-73. PMID: 12917338
  6. 子宫内膜癌中的细胞周期蛋白 D1 基因（CCND1）突变。  |  Moreno-Bueno, G., et al. 2003. Oncogene. 22: 6115-8. PMID: 12955092
  7. 细胞周期蛋白 D1 诱导细胞迁移需要 p27(KIP1)。  |  Li, Z., et al. 2006. Cancer Res. 66: 9986-94. PMID: 17047061
  8. 细胞周期蛋白 D1 调节肝脏雌激素和雄激素代谢  |  Mullany, LK., et al. 2010. Am J Physiol Gastrointest Liver Physiol. 298: G884-95. PMID: 20338923
  9. 细胞周期蛋白 D1 失活可延长增殖并改变出生后小鼠视网膜的组织发生。  |  Das, G., et al. 2012. Dev Dyn. 241: 941-52. PMID: 22434780
  10. 小淋巴细胞淋巴瘤/慢性淋巴细胞白血病增殖中心的细胞周期蛋白 D1 过表达。  |  Teixeira Mendes, LS., et al. 2017. J Clin Pathol. 70: 899-902. PMID: 28408436
  11. 鉴定人类细胞有丝分裂中的细胞周期蛋白A/细胞周期蛋白依赖性激酶1底物。  |  Dumitru, AMG. and Compton, DA. 2022. Methods Mol Biol. 2415: 175-182. PMID: 34972954
  12. 细胞周期蛋白 D1（CCND1、PRAD1）在人类癌细胞中的过表达机制：等位基因特异性表达分析。  |  Hosokawa, Y. and Arnold, A. 1998. Genes Chromosomes Cancer. 22: 66-71. PMID: 9591636

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin D1 抗体 (A-12)

sc-8396
200 µg/ml
RMB2377.00

cyclin D1 (A-12): m-IgG2b BP-HRP 套装

sc-548643
200 µg Ab; 10 µg BP
RMB2662.00

cyclin D1 抗体 (A-12) AC

sc-8396 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin D1 抗体 (A-12) HRP

sc-8396 HRP
200 µg/ml
RMB2377.00

cyclin D1 抗体 (A-12) FITC

sc-8396 FITC
200 µg/ml
RMB2482.00

cyclin D1 抗体 (A-12) PE

sc-8396 PE
200 µg/ml
RMB2580.00

cyclin D1 抗体 (A-12) Alexa Fluor® 488

sc-8396 AF488
200 µg/ml
RMB2685.00

cyclin D1 抗体 (A-12) Alexa Fluor® 546

sc-8396 AF546
200 µg/ml
RMB2685.00

cyclin D1 抗体 (A-12) Alexa Fluor® 594

sc-8396 AF594
200 µg/ml
RMB2685.00

cyclin D1 抗体 (A-12) Alexa Fluor® 647

sc-8396 AF647
200 µg/ml
RMB2685.00

cyclin D1 抗体 (A-12) Alexa Fluor® 680

sc-8396 AF680
200 µg/ml
RMB2685.00

cyclin D1 抗体 (A-12) Alexa Fluor® 790

sc-8396 AF790
200 µg/ml
RMB2685.00

cyclin D1 抗体 (A-12) TRITC

sc-8396 TRITC
200 µg/ml
RMB2407.00

DILUTION FOR IHC ?

Asked by: MIMO
Thank you for your question. Dilution recommendations can be found in the antibody's datasheet, which is available for download at the top of this page. For IHC (including paraffin-embedded sections) we recommend a starting dilution of 1:50, with a dilution range between 1:50-1:500.
Answered by: Tech Support Europe
Date published: 2023-05-22

Can I know this antibody epitope binding site? sc-8396

Asked by: CyclinD1
Thank you for your question. We can only show related immunogenic information, it is raised against amino acids 1-295 representing full length cyclin D1 of human origin.
Answered by: Ling Chen
Date published: 2022-06-14

I am looking for a CDK1 antibody suitable to detect a C-terminal GFP CDK1 fusion protein on WB, where CDK1 is truncated resulting in a loss of the last 5 amino acids of CDK1. Which antibody would you recommend? Thanks!

Asked by: Sebastian Scheich
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-02-04

What is the recommended secondary detection reagent for cyclin D1 Antibody (A-12) in Western blot applications?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-29
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Rated 5 out of 5 by from 表现出色我们使用了这款Cyclin D1抗体进行Western Blot和免疫荧光实验，效果非常出色。在Western Blot实验中，抗体表现出高特异性，信号清晰，背景低，能够准确检测到目标蛋白。在免疫荧光实验中，抗体的染色效果非常稳定，标记非常清晰，细胞定位结果一致。
Date published: 2024-12-19
Rated 4 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-19
Rated 5 out of 5 by from Western BlotWork very nicely for WB in MCF-7 cells treated for 24 hours with estrogen.
Date published: 2023-02-28
Rated 5 out of 5 by from Perfect bands in WBI have been using this antibody for a quite some time now. It gives a very clear band at the correct molecular weight when tested in human and murine cell lysates.
Date published: 2022-12-02
Rated 4 out of 5 by from WORKS WELL FOR WBIt is good for western blot.(cell lysates :K562....)
Date published: 2022-08-15
Rated 4 out of 5 by from Ciclin D1 antibodyWorks excellent in western blot for human Cyclin D1 detection
Date published: 2021-06-02
Rated 4 out of 5 by from Works for WBIt works good for detecting rat liver protein by western blot
Date published: 2021-01-18
Rated 5 out of 5 by from 功能很出色之前用过别的家的抗体不行，换了这家的cyclin D1，结果一下子就出来了.很给力，希望更多人受益。
Date published: 2021-01-05
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cyclin D1 抗体 (A-12) is rated 4.3 out of 5 by 73.
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