Date published: 2025-9-7
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cyclin D1 Antibody (72-13G) - Western Blotting - Image 379654
细胞周期蛋白 D1 抗体 (72-13G): sc-450. RAW 264. 7 (A), J774. A1 (B), TK-1 (C) 和 A-10 (D) 全细胞裂解液中细胞周期蛋白 D1 表达的 Western 印迹分析.
细胞周期蛋白 D1 抗体 (72-13G): sc-450. RAW 264. 7 (A), J774. A1 (B), TK-1 (C) 和 A-10 (D) 全细胞裂解液中细胞周期蛋白 D1 表达的 Western 印迹分析.

cyclin D1 抗体 (72-13G): sc-450

4.2(20)
寫評論提問

说明书
  • cyclin D1抗体(72-13G)是小鼠单克隆IgG1 κ, 在516篇文献中引用，规格为200 µg/ml
  • 针对mouse来源的重组融合蛋白的抗原决定簇
  • 抗-cyclin D1 抗体 (72-13G) 推荐用于 WB, IP, IF, IHC(P) 和 KA，检测mouse, rat 和human 来源的 cyclin D1; 可能与 cyclin D2 or cyclin D3 encoded proteins产生交联反应
  • 抗cyclin D1抗体（72-13G）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cyclin D1 (72-13G): sc-450的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 cyclin D1 抗体 (72-13G) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cyclin D1 抗体 (72-13G) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Cyclin抗体，包括cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2和cyclin D3

Cyclin D1抗体（72-13G）是一种鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和激酶分析等方法检测鼠、鼠和人源的Cyclin D1蛋白。Cyclin D1 (72-13G) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。细胞周期蛋白D1在调节细胞周期中起着关键作用，特别是在从G1期到S期的过渡期，这一时期对于细胞增殖和生长至关重要。细胞周期蛋白D1通过与细胞周期依赖性激酶（CDKs）形成复合物发挥作用，特别是CDK4和CDK6，使视网膜母细胞瘤蛋白（Rb）磷酸化。Rb磷酸化导致E2F转录因子的释放，而E2F转录因子对于细胞周期进展和DNA合成所需的基因转录至关重要。细胞周期蛋白D1的失调与多种癌症有关，因为其过度表达会导致细胞分裂失控，因此细胞周期蛋白D1成为癌症研究和治疗开发的重要研究对象。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin D1 抗体 (72-13G)

sc-450
200 µg/ml
$316.00

cyclin D1 (72-13G): m-IgG Fc BP-HRP 套装

sc-526459
200 µg Ab; 10 µg BP
$354.00

cyclin D1 (72-13G): m-IgG1 BP-HRP 套装

sc-531832
200 µg Ab; 20 µg BP
$354.00

cyclin D1 抗体 (72-13G) AC

sc-450 AC
500 µg/ml, 25% agarose
$416.00

cyclin D1 抗体 (72-13G) HRP

sc-450 HRP
200 µg/ml
$316.00

cyclin D1 抗体 (72-13G) FITC

sc-450 FITC
200 µg/ml
$330.00

cyclin D1 抗体 (72-13G) PE

sc-450 PE
200 µg/ml
$343.00

cyclin D1 抗体 (72-13G) Alexa Fluor® 488

sc-450 AF488
200 µg/ml
$357.00

cyclin D1 抗体 (72-13G) Alexa Fluor® 546

sc-450 AF546
200 µg/ml
$357.00

cyclin D1 抗体 (72-13G) Alexa Fluor® 594

sc-450 AF594
200 µg/ml
$357.00

cyclin D1 抗体 (72-13G) Alexa Fluor® 647

sc-450 AF647
200 µg/ml
$357.00

cyclin D1 抗体 (72-13G) Alexa Fluor® 680

sc-450 AF680
200 µg/ml
$357.00

cyclin D1 抗体 (72-13G) Alexa Fluor® 790

sc-450 AF790
200 µg/ml
$357.00

Hello, I am wondering about the epitope for the sc-450 antibody. thank you. Masha

Asked by: Masha Brooun
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-04-11

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
While this antibody is recommended for IHC, best results of mouse monoclonal antibody staining on mouse tissue, come from directly conjugated antibodies. See cyclin D1 (A-12): sc-8396 for cyclin D1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Tech Service 5
Date published: 2017-01-05
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Rated 3 out of 5 by from Cyclin D1 AntibodyGood WB results obtained, but not very clean and the expression of the band is weak at a dilution 1: 500
Date published: 2021-12-15
Rated 1 out of 5 by from NO BANDSUnfortunately, I did not get any bands in LNCaP cell line even at 1:100 dilution.
Date published: 2020-11-05
Rated 5 out of 5 by from Pulito e dall'ottimo funzionamentoHo acquistato questo articolo quasi un anno fa ed utilizzato in diversi saggi di Western Blot ha sempre dato un'ottima resa.
Date published: 2020-10-23
Rated 2 out of 5 by from Weak Western detection in rat INS-1 lysates60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a faint band of ~38 kD. Recommend using sc-20044 instead. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 1 out of 5 by from no band in my membraneno band in my membrane. the service after sold of santa cruz is more perfect than other compay suh as abcam and CST which there are a lot of requirment if you want to return the product.
Date published: 2018-04-22
Rated 4 out of 5 by from a good antibody for WBThis antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). although the intensity is not very strong. For WB, I would recommend other products such as sc-8396 or sc-20044.
Date published: 2017-09-18
Rated 3 out of 5 by from this antibody can be used in WB.I use it in WB . SK-N-SH cell lysis used。 10% gel，electrophoresis： 30 mA,90 min。 transfer membrane ：300 ma，45min。5% milk block，1：:100 antibody overnight。1X TTBS wash 3 times ，second antibody 1:2000 ，1 h ， temperature。then 1X TTBS wash 3 times。 15 min can appearance bands sometimes。but there is a specific band。
Date published: 2017-08-31
Rated 5 out of 5 by from work wellcyclin D1 work well for wb. It suitable for detect endogenous cyclin D1 in primary murine macrophages as it should according to datasheet by WB. It got satisfied band.
Date published: 2017-08-19
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cyclin D1 抗体 (72-13G) is rated 4.2 out of 5 by 20.
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