Date published: 2025-9-8

021-6093-6350

SCBT Portrait Logo
Seach Input

cyclin B 抗体 (D-1): sc-166210

4.1(9)
寫評論提問

说明书
  • cyclin B 抗体 D-1 是小鼠单克隆 IgG1 κ,cyclin B抗体, 在20篇文献中引用,规格为200 µg/ml
  • 免疫Drosophila melanogaster物种的cyclin B的氨基酸1-300
  • cyclin B 抗体 (D-1) 推荐用于WB, IP, IF 和 ELISA,检测d 来源的 cyclin B
  • 抗cyclin B抗体(D-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cyclin B (D-1): sc-166210免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是cyclin B Antibody (D-1) for WB applications. 的首选辅助检测试剂,这些试剂现与cyclin B Antibody (D-1) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

cyclin B抗体(D-1)是一种IgG1 κ小鼠单克隆cyclin B抗体(也称为CCNB抗体),可通过WB、IP、IF和ELISA检测黑腹果蝇来源的cyclin B蛋白。cyclin B抗体(D-1)既可以作为非偶联的抗cyclin B抗体形式提供,也可以作为多种偶联形式的抗cyclin B抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。黑腹果蝇已被证明是研究高等真核生物共有的发育和细胞过程的有效模型。从超过120兆碱基的常染色质中已阐明了大约13600个基因,它们分布在2号、3号、4号、X和Y染色体上,其中Y染色体主要是异染色质。果蝇的基因可以根据它们所编码的蛋白质类型进行分类,主要有六大类,包括细胞内信号蛋白、跨膜蛋白、RNA结合蛋白、分泌因子、转录调节因子(包括基本螺旋-环-螺旋、含同源结构域、含锌指和染色质相关)或其他功能蛋白。细胞周期蛋白是一个多样化的蛋白质家族,其定义特征是它们结合并激活细胞周期蛋白依赖性激酶(Cdk)家族成员并影响细胞周期控制。果蝇的细胞周期蛋白A和B都调节细胞周期蛋白依赖性激酶cdc2,其中细胞周期蛋白A的表达在前期达到高峰,而细胞周期蛋白B的表达则在中期之前达到高峰。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin B 抗体 (D-1) 参考文献:

  1. 细胞周期蛋白和细胞周期检查点  |  Johnson, DG. and Walker, CL. 1999. Annu Rev Pharmacol Toxicol. 39: 295-312. PMID: 10331086
  2. 黑腹果蝇的基因组序列。  |  Adams, MD., et al. 2000. Science. 287: 2185-95. PMID: 10731132
  3. 抗酶是果蝇生殖细胞中性致死基因的一个靶标,似乎在刺猬的下游起着调节性致死基因和细胞周期蛋白 B 的作用。  |  Vied, C., et al. 2003. Dev Biol. 253: 214-29. PMID: 12645926
  4. 果蝇细胞周期蛋白 B 转录本中的 3' 非翻译序列在卵子发生后期引导后极聚集,并在合胞胚胎中引导核周联合。  |  Dalby, B. and Glover, DM. 1992. Development. 115: 989-97. PMID: 1451670
  5. 细胞周期蛋白 B 水平和 DNA 复制检查点都控制着果蝇早期胚胎的有丝分裂。  |  Ji, JY., et al. 2004. Development. 131: 401-11. PMID: 14681192
  6. 果蝇幼虫大脑中 N 端截短的细胞周期蛋白 B 的表达会导致无丝分裂后期的有丝分裂延迟。  |  Rimmington, G., et al. 1994. J Cell Sci. 107 (Pt 10): 2729-38. PMID: 7876341
  7. DNA 复制的细胞周期控制  |  Fotedar, R. and Fotedar, A. 1995. Prog Cell Cycle Res. 1: 73-89. PMID: 9552354

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin B 抗体 (D-1)

sc-166210
200 µg/ml
RMB2377.00

cyclin B (D-1): m-IgG Fc BP-HRP 套装

sc-527144
200 µg Ab; 10 µg BP
RMB2662.00

cyclin B (D-1): m-IgG1 BP-HRP 套装

sc-532517
200 µg Ab; 20 µg BP
RMB2662.00

cyclin B 抗体 (D-1) AC

sc-166210 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin B 抗体 (D-1) HRP

sc-166210 HRP
200 µg/ml
RMB2377.00

cyclin B 抗体 (D-1) FITC

sc-166210 FITC
200 µg/ml
RMB2482.00

cyclin B 抗体 (D-1) PE

sc-166210 PE
200 µg/ml
RMB2580.00

cyclin B 抗体 (D-1) Alexa Fluor® 488

sc-166210 AF488
200 µg/ml
RMB2685.00

cyclin B 抗体 (D-1) Alexa Fluor® 546

sc-166210 AF546
200 µg/ml
RMB2685.00

cyclin B 抗体 (D-1) Alexa Fluor® 594

sc-166210 AF594
200 µg/ml
RMB2685.00

cyclin B 抗体 (D-1) Alexa Fluor® 647

sc-166210 AF647
200 µg/ml
RMB2685.00

cyclin B 抗体 (D-1) Alexa Fluor® 680

sc-166210 AF680
200 µg/ml
RMB2685.00

cyclin B 抗体 (D-1) Alexa Fluor® 790

sc-166210 AF790
200 µg/ml
RMB2685.00

What blocking solution should I use with cyclin B (D-1): sc-166210 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_166210, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Very specific antibodyThis antibody works well with my project! No non-specific bands.
Date published: 2020-02-24
Rated 4 out of 5 by from Good featuresGood quality antibody for western blot detection of cyclin B in cell lysates with visible difference between proliferating and growth arrested cells
Date published: 2018-03-12
Rated 3 out of 5 by from an OK antibody for WBThis antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), albeit the intensity is somewhat weak
Date published: 2017-09-18
Rated 4 out of 5 by from not suitable for turbotthere is a very light band when i exposed for 25min
Date published: 2017-07-10
Rated 1 out of 5 by from Didnot work for WBNo band was observed in MCF-7 cell lysates. Used the ab at 1:1000 dilution.
Date published: 2017-07-03
Rated 5 out of 5 by from Great featureThis antibody has been tested by WB for HepG2 cells. I used the product and diluted in TBST(1:200),4℃ overnight.
Date published: 2017-06-02
Rated 5 out of 5 by from show good resultIn my Western blot analysis,this cyclinB antibody show good performance.The band is very strong.
Date published: 2016-12-05
Rated 5 out of 5 by from It worked very nice on WB with Drosophila tissuesIt worked very nice on WB with Drosophila tissues.
Date published: 2015-05-19
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_166210, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
cyclin B 抗体 (D-1) is rated 4.1 out of 5 by 9.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_166210, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 120ms
  • REVIEWS, PRODUCT