Date published: 2025-9-7

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cyclin A 抗体 (BF683): sc-239

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说明书
  • cyclin A 抗体 BF683 是小鼠单克隆 IgE, 在288篇文献中引用,提供 200 µg/ml
  • 针对全长重组蛋白cyclin A的抗体,来源于human
  • 推荐用于 mouse, rat 和 human 来源的cyclin A WB, IP, IF, FCM, KA 和 ELISA检测
  • 另提供琼脂糖偶联抗体,规格为可用于IP实验;以及藻红蛋白 偶联抗体,规格为 用于IF、IHC(P)和FCM实验
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-239 X, 200 µg/0.1 ml)
  • 看 cyclin A (B-8): sc-271682,可查看有标记的 cyclin A 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取cyclin A (BF683): sc-239免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是cyclin A Antibody (BF683) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与cyclin A Antibody (BF683) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

cyclin A抗体(BF683)是一种IgE小鼠单克隆cyclin A抗体,可通过WB、IP、IF、FCM、KA和ELISA检测小鼠、大鼠和人源cyclin A。cyclin A抗体(BF683)既可作为偶联抗cyclin A抗体形式使用,也可作为非偶联抗cyclin A抗体形式使用。调节蛋白家族(称为细胞周期蛋白)在真核细胞周期调节中的关键作用已得到充分证实。表征最好的细胞周期蛋白复合物是有丝分裂细胞周期蛋白B/Cdc2 p34激酶,这是成熟促进因子的活性成分。细胞周期蛋白A在细胞周期中先于细胞周期蛋白B积累,似乎参与S期的控制,并已显示与细胞周期蛋白依赖性激酶-2(Cdk2)相关联。此外,细胞周期蛋白A已被证实参与细胞转化,并与E1A、转录因子DRTF1和E2F以及视网膜母细胞瘤蛋白p110形成复合物。第二种形式的细胞周期蛋白A被称为细胞周期蛋白A1,因为它与非洲爪蟾细胞周期蛋白A1具有高度序列同源性,在生殖细胞中的表达最高。有人提出,细胞周期蛋白A1可以与Cdk2、p39和Cdc2 p34结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin A 抗体 (BF683) 参考文献:

  1. 细胞周期蛋白 E/cdk2 和细胞周期蛋白 A/cdk2 激酶以不同的时间方式与 p107 和 E2F 结合。  |  Lees, E., et al. 1992. Genes Dev. 6: 1874-85. PMID: 1398067
  2. p34cdc2/p58cyclin A脯氨酸导向蛋白激酶和视网膜母细胞瘤肿瘤易感基因产物的共同纯化:致癌丝氨酸/苏氨酸蛋白激酶与肿瘤抑制蛋白的相互作用。  |  Williams, RT., et al. 1992. Oncogene. 7: 423-32. PMID: 1532245
  3. 细胞周期蛋白 A 和视网膜母细胞瘤基因产物与一个共同的转录因子复合。  |  Bandara, LR., et al. 1991. Nature. 352: 249-51. PMID: 1830372
  4. 肝细胞癌中细胞周期蛋白 A 基因中的乙型肝炎病毒整合。  |  Wang, J., et al. 1990. Nature. 343: 555-7. PMID: 1967822
  5. Cyclin 是爪蟾成熟促进因子的一种成分。  |  Gautier, J., et al. 1990. Cell. 60: 487-94. PMID: 1967981
  6. Cyclin A的乙酰化:一种新的细胞周期调节机制。  |  Mateo, F., et al. 2010. Biochem Soc Trans. 38: 83-6. PMID: 20074040
  7. 人类细胞周期蛋白 A 是腺病毒 E1A 相关蛋白 p60,其行为与细胞周期蛋白 B 不同。  |  Pines, J. and Hunter, T. 1990. Nature. 346: 760-3. PMID: 2143810
  8. Cdc2 蛋白激酶与细胞周期蛋白 A 和 B 复合物:MPF 蛋白水解失活的证据。  |  Draetta, G., et al. 1989. Cell. 56: 829-38. PMID: 2538242
  9. 一种 60 kd 的 cdc2- 相关多肽与腺病毒感染细胞中的 E1A 蛋白复合。  |  Giordano, A., et al. 1989. Cell. 58: 981-90. PMID: 2570639
  10. ORC1-Cyclin A结合的结构基础。  |  Wang, B. and Song, J. 2019. Protein Sci. 28: 1727-1733. PMID: 31309634
  11. 在睾丸和几种白血病细胞系中高表达的第二种人类细胞周期蛋白 A 的特征。  |  Yang, R., et al. 1997. Cancer Res. 57: 913-20. PMID: 9041194
  12. Cyclin A:细胞增殖过程中的功能和表达。  |  Desdouets, C., et al. 1995. Prog Cell Cycle Res. 1: 115-23. PMID: 9552357

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin A 抗体 (BF683)

sc-239
200 µg/ml
RMB2377.00

cyclin A (BF683): m-IgGκ BP-HRP 套装

sc-551812
200 µg Ab; 40 µg BP
RMB2662.00

cyclin A 抗体 (BF683) AC

sc-239 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin A 抗体 (BF683) PE

sc-239 PE
200 µg/ml
RMB2580.00

cyclin A 抗体 (BF683) X

sc-239 X
200 µg/0.1 ml
RMB2377.00

When should the TransCruz reagent sc-293 X be used instead of the standard form sc-293?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 14
Date published: 2017-01-09
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Rated 5 out of 5 by from WB结果很好3年前申请了小样,用过感觉不错,便购买了,1:800稀释用于WB实验,条带无背景,很清晰,强烈推荐!
Date published: 2025-02-15
Rated 1 out of 5 by from No Detection In INS-1 Rat CellsTested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Presence of CcnA protein in this cell line was previously confirmed using discontinued product sc-596. Product has not been personally tested in human or mouse lines.
Date published: 2019-05-22
Rated 4 out of 5 by from The antibody is goodThe antibody was used to examine the protein of cyclin A in rat tissues by western blotting method. The bands were sepcific.
Date published: 2018-11-14
Rated 5 out of 5 by from Great product!!It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 4 out of 5 by from a good antibody for WBThis antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, the signal intensity is not as good as that observed for sc-271682 in our hands.
Date published: 2017-09-18
Rated 4 out of 5 by from Works well!Very specific western blot results; strong clear signal
Date published: 2017-06-07
Rated 5 out of 5 by from WBI repeated the WB several times with this antibody, each time the band was clear
Date published: 2017-06-05
Rated 5 out of 5 by from Best cyclin A antibodyNice FCM data and strong western blot results !!!!
Date published: 2017-01-10
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cyclin A 抗体 (BF683) is rated 4.5 out of 5 by 15.
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