Date published: 2025-9-11
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Cya A Antibody (3D1) - Western Blotting - Image 6508
Cya A 抗体 (3D1): sc-13582. 正品腺苷酸环化酶毒素的 Western 印迹分析.
Cya A Antibody (3D1) - Western Blotting - Image 6508
Cya A 抗体 (3D1): sc-13582. 正品腺苷酸环化酶毒素的 Western 印迹分析.
Cya A 抗体 (3D1): sc-13582. 正品腺苷酸环化酶毒素的 Western 印迹分析.

Cya A 抗体 (3D1): sc-13582

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说明书
  • Cya A 抗体 3D1 是小鼠单克隆 IgG1 κ，Cya A抗体, 在74篇文献中引用，规格为200 µg/ml
  • 培养自Bordetella pertussis腺苷酸环化酶毒素来源的1-400氨基酸
  • Cya A 抗体 (3D1) 推荐用于WBIP，检测B. pertussis 来源的 Cya A
  • 抗Cya A抗体（3D1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cya A (3D1): sc-13582的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Cya A Antibody (3D1) for WB applications. 的首选辅助检测试剂，这些试剂现与Cya A Antibody (3D1) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

Cya A抗体（3D1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测百日咳杆菌来源的Cya A蛋白。Cya A (3D1)抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Cya A 是一种蛋白质，也称为腺苷酸环化酶毒素，在百日咳的发病机理中起着关键作用，它通过提高宿主细胞中的细胞内环状腺苷酸（cAMP）水平，导致细胞毒性效应。这种升高会破坏正常的细胞信号传导通路，而该通路对于细菌逃避宿主免疫反应的能力至关重要。Cya A基因是一个更大基因座的一部分，该基因座编码多种转运和激活所需的蛋白质，包括将原毒素转化为活性形式所必需的Cya C。此外，Cya A与αMβ2整合素（CD11b/CD18）相互作用，后者主要存在于免疫细胞（如中性粒细胞和巨噬细胞）上，从而促进细菌进入这些细胞并破坏先天免疫反应。了解Cya A的功能和相互作用对于制定针对百日咳杆菌感染的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cya A 抗体 (3D1) 参考文献:

  1. 腺苷酸环化酶毒素的中和抗体可促进人类中性粒细胞对百日咳博德特氏菌的吞噬作用。  |  Weingart, CL., et al. 2000. Infect Immun. 68: 7152-5. PMID: 11083845
  2. 百日咳杆菌的腺苷酸环化酶毒素通过α（M）β（2）整合素（CD11b/CD18）与靶细胞结合。  |  Guermonprez, P., et al. 2001. J Exp Med. 193: 1035-44. PMID: 11342588
  3. 溶血不需要百日咳杆菌腺苷酸环化酶毒素的酶活性。  |  Ehrmann, IE., et al. 1992. FEBS Lett. 304: 51-6. PMID: 1319923
  4. 消除百日咳杆菌双功能 cyaA 毒素的腺苷酸环化酶或溶血素功能的靶向突变可消除毒性。  |  Gross, MK., et al. 1992. Proc Natl Acad Sci U S A. 89: 4898-902. PMID: 1594590
  5. 在重建的大肠杆菌系统中高水平合成百日咳杆菌的活性腺苷酸环化酶毒素。  |  Sebo, P., et al. 1991. Gene. 104: 19-24. PMID: 1916273
  6. 腺苷酸环化酶毒素促进整合素和浮岛成分的内吞作用，并降低巨噬细胞的粘附能力。  |  Martín, C., et al. 2011. PLoS One. 6: e17383. PMID: 21383852
  7. 腺苷酸环化酶毒素介导的百日咳毒素S1亚基在哺乳动物细胞中的传递。  |  Iwaki, M. and Konda, T. 2016. Pathog Dis. 74: ftv110. PMID: 26607401
  8. 腺苷酸环化酶毒素的磷脂酶A活性介导其腺苷酸环化酶结构域的易位。  |  González-Bullón, D., et al. 2017. Proc Natl Acad Sci U S A. 114: E6784-E6793. PMID: 28760979
  9. 博德特氏菌腺苷酸环化酶毒素的膜渗透作用涉及可调节大小的孔。  |  González-Bullón, D., et al. 2019. Biomolecules. 9: PMID: 31083482
  10. 博德特氏菌腺苷酸环化酶毒素抑制单核细胞向巨噬细胞转化并使人肺泡巨噬细胞脱分化为单核细胞样细胞  |  Ahmad, JN., et al. 2019. mBio. 10: PMID: 31551332
  11. 百日咳博德特氏菌腺苷酸环化酶毒素（CyaA）在脂质双分子层膜中的膜活性和通道形成。  |  Knapp, O. and Benz, R. 2020. Toxins (Basel). 12: PMID: 32164365
  12. 百日咳杆菌腺苷酸环化酶毒素：在大肠杆菌中分别合成的 proCyaA 和 CyaC 蛋白在体外产生活性毒素。  |  Westrop, GD., et al. 1996. Gene. 180: 91-9. PMID: 8973351

订购信息

产品名称产品编号规格价格数量收藏夹

Cya A 抗体 (3D1)

sc-13582
200 µg/ml
$316.00

Cya A (3D1): m-IgG Fc BP-HRP 套装

sc-526562
200 µg Ab; 10 µg BP
$354.00

Cya A (3D1): m-IgG1 BP-HRP 套装

sc-531935
200 µg Ab; 20 µg BP
$354.00

Cya A 抗体 (3D1) AC

sc-13582 AC
500 µg/ml, 25% agarose
$416.00

Cya A 抗体 (3D1) HRP

sc-13582 HRP
200 µg/ml
$316.00

Cya A 抗体 (3D1) FITC

sc-13582 FITC
200 µg/ml
$330.00

Cya A 抗体 (3D1) PE

sc-13582 PE
200 µg/ml
$343.00

Cya A 抗体 (3D1) Alexa Fluor® 488

sc-13582 AF488
200 µg/ml
$357.00

Cya A 抗体 (3D1) Alexa Fluor® 546

sc-13582 AF546
200 µg/ml
$357.00

Cya A 抗体 (3D1) Alexa Fluor® 594

sc-13582 AF594
200 µg/ml
$357.00

Cya A 抗体 (3D1) Alexa Fluor® 647

sc-13582 AF647
200 µg/ml
$357.00

Cya A 抗体 (3D1) Alexa Fluor® 680

sc-13582 AF680
200 µg/ml
$357.00

Cya A 抗体 (3D1) Alexa Fluor® 790

sc-13582 AF790
200 µg/ml
$357.00

I have this antibody (old) Lot E1716. I performed a Bacterial two-hybrid assay. Can I use this to detect T18 fragment? I saw the upper message: Clear detection at 25 kDa. But nothing is about T18 fragment.

Asked by: Dr Deepak Anand
Thank you for your question. According to the following publication on PubMed (PMID: 16948859) this clone binds to the distal portion – amino acids 373–399 – of fragment T18.
Answered by: Technical Support
Date published: 2022-01-06

Which Cya A antibody do you recommend for use in Western blot?

Asked by: cjMara
Although we offer a few different clones for Cya A, the 3D1 clone is the most popular and has been featured in over 15 recent publications.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from Clear detection at 25 kdaanti-3D1 (sc-13582) and anti-HRP (sc-2005). You can ignore the 2nd lane and last lanes; prestained ladder
Date published: 2017-02-07
Rated 5 out of 5 by from Great product!!Very nice wester blot data; strong signal with low background
Date published: 2017-01-26
Rated 5 out of 5 by from Great antibody for WesternsThis antibody is great for WB and always shows strong signal with little background.
Date published: 2017-01-10
Rated 4 out of 5 by from Published data for WB in plant Psy61 andPublished data for WB in plant Psy61 and Psy61 cells. -SCBT Publication Review
Date published: 2015-04-06
Rated 4 out of 5 by from Published data for WB in human INT 407 cellsPublished data for WB in human INT 407 cells. -SCBT Publication Review
Date published: 2015-03-22
Rated 4 out of 5 by from Published data for WB in human THP1 cellsPublished data for WB in human THP1 cells. -SCBT Publication Review
Date published: 2015-03-18
Rated 4 out of 5 by from Published data for WB in YPublished data for WB in Y. enterocolitica. -SCBT Publication Review
Date published: 2015-02-21
Rated 4 out of 5 by from Published data for WB in LPublished data for WB in L. pneumophila JR32 and E. coli MC1022. -SCBT Publication Review
Date published: 2015-01-04
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Cya A 抗体 (3D1) is rated 4.3 out of 5 by 9.
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