Date published: 2025-9-8
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CtBP Antibody (E-12) - Western Blotting - Image 354375
CtBP 抗体 (E-12): sc-17759. 在 SH-SY5Y (A), Neuro-2A (B), Sol8 (C), 3T3-L1 (D), C6 (E) 和 NRK (F) 全细胞裂解液中 CtBP 表达的 Western 印迹分析.
CtBP 抗体 (E-12): sc-17759. 在 SH-SY5Y (A), Neuro-2A (B), Sol8 (C), 3T3-L1 (D), C6 (E) 和 NRK (F) 全细胞裂解液中 CtBP 表达的 Western 印迹分析.

CtBP 抗体 (E-12): sc-17759

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说明书
  • CtBP 抗体 E-12 是小鼠单克隆 IgG1 κ，CtBP抗体, 在42篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CtBP1的氨基酸1-440
  • CtBP 抗体 (E-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CtBP1 and CtBP2
  • 抗CtBP抗体（E-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CtBP (E-12): sc-17759的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CtBP 抗体 (E-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CtBP 抗体 (E-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CtBP 抗体包括 和 CtBP1

CtBP抗体（E-12）是一种鼠单克隆IgG1 kappa轻链抗体，可通过应用如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的CtBP蛋白。CtBP (E-12) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。CtBP1 单克隆抗体 (E-12) 可识别一种关键的细胞磷蛋白，该蛋白可作为转录共抑制因子，调节基因表达并维持细胞内环境稳定。CtBP1 (E-12) 抗体靶向δEF1（一种锌指-同源结构域蛋白）的相互作用蛋白，在骨骼和T细胞发育过程中增强转录沉默。CtBP1与CtIP形成复合物，后者是一种与乳腺癌基因BRCA1的BRCT重复序列结合的蛋白，影响BRCA1的活性。CtBP1抑制p21启动子的转激活，而p21启动子是细胞周期和DNA损伤反应的关键调节因子，这表明其在细胞应激反应中的重要性，以及潜在的癌症生物学意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CtBP 抗体 (E-12) 参考文献:

  1. CtIP 与参与 p21 转录调控的 BRCA1 的 BRCT 重复序列的结合在 DNA 损伤时被破坏。  |  Li, S., et al. 1999. J Biol Chem. 274: 11334-8. PMID: 10196224
  2. 确定 CtBP1 和 CtBP2 为锌指-homeodomain 因子 deltaEF1 的核心加压因子。  |  Furusawa, T., et al. 1999. Mol Cell Biol. 19: 8581-90. PMID: 10567582
  3. 一种新型核心抑制因子 BCoR-L1 通过与 CtBP 的相互作用抑制转录。  |  Pagan, JK., et al. 2007. J Biol Chem. 282: 15248-57. PMID: 17379597
  4. CtBP--新兴的致癌基因和新型小分子药物靶点：加深对其致癌作用的理解并确定治疗抑制剂。  |  Dcona, MM., et al. 2017. Cancer Biol Ther. 18: 379-391. PMID: 28532298
  5. 抑制 CtBP 复合物和 FBXO11 可增强 MHC II 类表达和抗癌免疫反应。  |  Chan, KL., et al. 2022. Cancer Cell. 40: 1190-1206.e9. PMID: 36179686
  6. CtBP二聚体状态的代谢调节会影响DNA损伤修复基因的抑制作用和卵巢癌对铂的敏感性。  |  Li, J., et al. 2023. Int J Biol Sci. 19: 2081-2096. PMID: 37151877
  7. 腺病毒 E1A 蛋白中的 CtBP 结合域控制着 CR1 依赖性反式激活。  |  Sollerbrant, K., et al. 1996. Nucleic Acids Res. 24: 2578-84. PMID: 8692699
  8. 一种与腺病毒 E1A（CtBP）C 端区域结合的细胞蛋白与一种新型细胞蛋白之间的相互作用被 E1A 通过一个保守的 PLDLS 基序破坏。  |  Schaeper, U., et al. 1998. J Biol Chem. 273: 8549-52. PMID: 9535825
  9. 抑制剂 deltaEF1 的两种作用机制：与激活剂竞争结合位点和主动抑制。  |  Sekido, R., et al. 1997. Genes Cells. 2: 771-83. PMID: 9544704
  10. mCtBP2 的克隆和特征描述，mCtBP2 是一种与碱性克鲁珀尔样因子及其他哺乳动物转录调节因子相关联的共抑制因子。  |  Turner, J. and Crossley, M. 1998. EMBO J. 17: 5129-40. PMID: 9724649
  11. BRCA1 的 C 端（BRCT）结构域在体内与 CtIP 相互作用，CtIP 是一种参与 CtBP 转录抑制途径的蛋白质。  |  Yu, X., et al. 1998. J Biol Chem. 273: 25388-92. PMID: 9738006
  12. 羧基末端 BRCA1 交互蛋白的特征。  |  Wong, AK., et al. 1998. Oncogene. 17: 2279-85. PMID: 9811458

订购信息

产品名称产品编号规格价格数量收藏夹

CtBP 抗体 (E-12)

sc-17759
200 µg/ml
RMB2377.00

CtBP (E-12): m-IgGκ BP-HRP 套装

sc-520639
200 µg Ab, 40 µg BP
RMB2662.00

CtBP (E-12): m-IgG1 BP-HRP 套装

sc-533468
200 µg Ab; 20 µg BP
RMB2662.00

CtBP 抗体 (E-12) AC

sc-17759 AC
500 µg/ml, 25% agarose
RMB3129.00

CtBP 抗体 (E-12) HRP

sc-17759 HRP
200 µg/ml
RMB2377.00

CtBP 抗体 (E-12) FITC

sc-17759 FITC
200 µg/ml
RMB2482.00

CtBP 抗体 (E-12) PE

sc-17759 PE
200 µg/ml
RMB2580.00

CtBP 抗体 (E-12) Alexa Fluor® 488

sc-17759 AF488
200 µg/ml
RMB2685.00

CtBP 抗体 (E-12) Alexa Fluor® 546

sc-17759 AF546
200 µg/ml
RMB2685.00

CtBP 抗体 (E-12) Alexa Fluor® 594

sc-17759 AF594
200 µg/ml
RMB2685.00

CtBP 抗体 (E-12) Alexa Fluor® 647

sc-17759 AF647
200 µg/ml
RMB2685.00

CtBP 抗体 (E-12) Alexa Fluor® 680

sc-17759 AF680
200 µg/ml
RMB2685.00

CtBP 抗体 (E-12) Alexa Fluor® 790

sc-17759 AF790
200 µg/ml
RMB2685.00

I am interested in this antibody. How could I get 10ug to try?

Asked by: Shilin
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-15

Can CtBP (E-12): sc-17759 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CtBP (E-12): sc-17759 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from CtBP: sc-17759 works in WB and is specificImage shows analysis of CtBP level in nonclonally selected population of LN229 cells upon CRISPR-Cas9-mediated KO CtBP using two different gRNAs. CtBP Antibody (E-12): sc-17759 detects a band of approximately 48 kDa (corresponding to molecular weight of CtBP) and is specific.
Date published: 2020-07-31
Rated 1 out of 5 by from It did not work for ChIP-seqIt did not work for ChIP-seq using 0.1% SDS in the lysis buffer. Same protocol worked well with another CTBP1 monoclonal ab from a different company.
Date published: 2019-11-22
Rated 5 out of 5 by from anti-CtBP Antibody.The antibody gives a nice and clean band for human CtBP in the human colorectal cancer cell line SW480
Date published: 2018-10-17
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of CtBP expression in SH-SY5Y, Neuro-2A, Sol8, 3T3-L1, C6 and NRK whole cell lysates.
Date published: 2017-01-19
Rated 5 out of 5 by from good experience with CtBP (E-12)I had good experience with sc-17759 (E-12). It is good for Western, IHC and co-IP. Based on product manual, this antibody (sc-17759) recognizes both CtBP1 and CtBP2. I did the CtBP1 and CtBP2 constructs and found this antibody (E-12) has a much higher affinity with CtBP1 than that with CtBP2, although both CtBP1 and CtBP2 are able to be recognized.
Date published: 2016-11-28
Rated 5 out of 5 by from Published for IPPublished for IP, WB and IF in human HEK and THP-1 cells. -SCBT Publication Review
Date published: 2015-06-30
Rated 5 out of 5 by from Strong pulldown in IP in human 293TStrong pulldown in IP in human 293T, HCT116, SW480, COLO320. -SCBT Publication Review
Date published: 2015-06-13
Rated 5 out of 5 by from Good specificity in WB in mouse transfectedGood specificity in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-20
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CtBP 抗体 (E-12) is rated 4.7 out of 5 by 12.
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