Date published: 2025-9-6
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Crk-L Antibody (B-1) - Western Blotting - Image 294405
Crk-L 抗体 (B-1): sc-365092. HeLa (A), A-431 (B) 和 NIH/3T3 (C) 全细胞裂解液中 Crk-L 表达的 Western 印迹分析.
Crk-L 抗体 (B-1): sc-365092. HeLa (A), A-431 (B) 和 NIH/3T3 (C) 全细胞裂解液中 Crk-L 表达的 Western 印迹分析.

Crk-L 抗体 (B-1): sc-365092

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说明书
  • Crk-L 抗体 B-1 是小鼠单克隆 IgG1 κ，Crk-L抗体, 在7篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Crk-L的氨基酸181-242
  • Crk-L 抗体 (B-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Crk-L
  • 抗Crk-L抗体（B-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Crk-L (B-1): sc-365092的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Crk-L Antibody (B-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Crk-L Antibody (B-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Crk抗体，包括Crk I, Crk II和Crk-L

Crk-L抗体（B-1）是一种IgG1 κ小鼠单克隆Crk-L抗体（也称为CRK样原癌基因抗体、适配体蛋白抗体、V-Crk禽肉瘤病毒CT10癌基因同源样抗体或Crk样蛋白抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Crk-L蛋白。Crk-L抗体（B-1）既有非偶联形式的抗Crk-L抗体，也有多种偶联形式的抗Crk-L抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SH2和SH3（Src同源）结构域最初被确定为具有酪氨酸激酶活性的非受体蛋白内的关键功能结构域。该家族中最早被确定的成员之一Crk是一种转化特异性蛋白，可诱导细胞磷酸酪氨酸水平升高，但其本身缺乏酪氨酸激酶活性。第二种蛋白质Nck仅由三个SH3结构域和一个SH2结构域组成，而GRB2则包含一个SH2结构域，其两侧各有一个SH3结构域。此类蛋白质的一个成员Crk-L由位于22号染色体、11号带、慢性髓性白血病断点区域着丝粒的基因编码。Crk-L编码一个含有1个SH2和2个SH3结构域的303个氨基酸的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Crk-L 抗体 (B-1) 参考文献:

  1. 含SH2和SH3结构域的蛋白质GRB2将受体酪氨酸激酶与ras信号传导联系起来。  |  Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
  2. Nck 对多种受体, 肉豆蔻酸缩醛和环 AMP 的磷酸化反应。  |  Park, D. and Rhee, SG. 1992. Mol Cell Biol. 12: 5816-23. PMID: 1333046
  3. SH2 和 SH3 结构域：从结构到功能。  |  Pawson, T. and Gish, GD. 1992. Cell. 71: 359-62. PMID: 1423600
  4. 酪氨酸磷酸化SOCS3与Nck和Crk-L适配器蛋白相互作用，并调节Nck的激活。  |  Sitko, JC., et al. 2004. J Biol Chem. 279: 37662-9. PMID: 15173187
  5. 受体和 Ras 之间的缺失环节。  |  Williams, LT. 1992. Curr Biol. 2: 601-3. PMID: 15336035
  6. 人类B淋巴瘤细胞表面EB病毒/C3d受体（gp140、CR2、CD21）的激活会引发Cbl酪氨酸磷酸化，使其与p85亚基、Crk-L和Syk结合，并与Vav分离。  |  Lottin-Divoux, S., et al. 2006. Cell Signal. 18: 1219-25. PMID: 16289966
  7. CD34与Crk-L的SH3结构域相互作用的3D结构预测。  |  Gangenahalli, GU., et al. 2005. Stem Cells Dev. 14: 470-7. PMID: 16305332
  8. SH2 和 SH3 结构域：控制细胞质信号蛋白相互作用的元件。  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
  9. Nck，一种黑色素瘤 cDNA，编码一种由 src 同源单元 SH2 和 SH3 组成的细胞质蛋白。  |  Lehmann, JM., et al. 1990. Nucleic Acids Res. 18: 1048. PMID: 2107526
  10. 免疫反应中的适配蛋白Crk。  |  Liu, D. 2014. Immunol Cell Biol. 92: 80-9. PMID: 24165979
  11. 与磷脂酶 C 结构相似的新型病毒癌基因  |  Mayer, BJ., et al. 1988. Nature. 332: 272-5. PMID: 2450282
  12. 人类 crk 样基因 CRKL 的分离和染色体定位。  |  ten Hoeve, J., et al. 1993. Oncogene. 8: 2469-74. PMID: 8361759

订购信息

产品名称产品编号规格价格数量收藏夹

Crk-L 抗体 (B-1)

sc-365092
200 µg/ml
RMB2377.00

Crk-L (B-1): m-IgG Fc BP-HRP 套装

sc-529308
200 µg Ab; 10 µg BP
RMB2662.00

Crk-L (B-1): m-IgGκ BP-HRP 套装

sc-522135
200 µg Ab, 40 µg BP
RMB2662.00

Crk-L (B-1): m-IgG1 BP-HRP 套装

sc-543444
200 µg Ab; 20 µg BP
RMB2662.00

Crk-L 抗体 (B-1) AC

sc-365092 AC
500 µg/ml, 25% agarose
RMB3129.00

Crk-L 抗体 (B-1) HRP

sc-365092 HRP
200 µg/ml
RMB2377.00

Crk-L 抗体 (B-1) FITC

sc-365092 FITC
200 µg/ml
RMB2482.00

Crk-L 抗体 (B-1) PE

sc-365092 PE
200 µg/ml
RMB2580.00

Crk-L 抗体 (B-1) Alexa Fluor® 488

sc-365092 AF488
200 µg/ml
RMB2685.00

Crk-L 抗体 (B-1) Alexa Fluor® 546

sc-365092 AF546
200 µg/ml
RMB2685.00

Crk-L 抗体 (B-1) Alexa Fluor® 594

sc-365092 AF594
200 µg/ml
RMB2685.00

Crk-L 抗体 (B-1) Alexa Fluor® 647

sc-365092 AF647
200 µg/ml
RMB2685.00

Crk-L 抗体 (B-1) Alexa Fluor® 680

sc-365092 AF680
200 µg/ml
RMB2685.00

Crk-L 抗体 (B-1) Alexa Fluor® 790

sc-365092 AF790
200 µg/ml
RMB2685.00

1) What is the cost of the AF647-conjugated antibody "sc-365092 AF647"? 2) Will this Crk-L antibody also stain Crk II?

Asked by: jmjerome
Thank you for your question. We are in the process of updating the pricing and ordering information for our conjugates on the website and this information should be available soon. The cost for sc-365092 AF647 is $314 for 200 µg in 1 ml. This monoclonal antibody is specific for Crk-L and is not expected to cross-react with Crk II.
Answered by: Technical Support
Date published: 2017-12-02

I want to know if the sc-365092: Crk-L (B-1) mouse monoclonal antibody can detect the phosphorylated Crkl? I know that anti-CRKL antibody (C-20, Santa Cruz) can detects the phosphorylated and unphosphorylated protein fractions,but it was off market.

Asked by: yanhua
Thank you for your question. Yes, this antibody would be able to detect both phosphorylated and non-phosphorylated Crk-L. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-23

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-365092: Crk-L (B-1) mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Good for WBWorks well in Western Blot with a variety of cell lysates at a 1:100 dilution with 50 ug of protein.
Date published: 2017-02-06
Rated 4 out of 5 by from Very good antibody for Western blottingThis product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a relatively potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It clearly detected an appropriately sized ~36 kDa target protein in these immunoblots, which is only slightly higher than the predicted molecular mass of 33.8 kDa for Crk-L.
Date published: 2016-10-13
Rated 5 out of 5 by from Nice Western Blot data of CrkNice Western Blot data of Crk-L expression in HeLa, A-431 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Great Western Blot data of CrkGreat Western Blot data of Crk-L expression in non-transfected 293T, human Crk-L transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2014-01-06
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Crk-L 抗体 (B-1) is rated 4.8 out of 5 by 4.
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