Date published: 2025-9-8
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COX5b Antibody (C-5) - Western Blotting - Image 414579
抗COX5b抗体（C-5）：sc-374416。Western blot分析HeLa（A）、K-562（B）和HCT-116（C）细胞裂解液中COX5b的表达。(C)。检测试剂：m-IgG Fc BP-HRP：sc-525409。
抗COX5b抗体（C-5）：sc-374416。Western blot分析HeLa（A）、K-562（B）和HCT-116（C）细胞裂解液中COX5b的表达。(C)。检测试剂：m-IgG Fc BP-HRP：sc-525409。

COX5b 抗体 (C-5): sc-374416

4.3(9)
寫評論提問

说明书
  • COX5b 抗体 C-5 是小鼠单克隆 IgG1 (kappa轻链)，COX5b抗体，规格为200 µg/ml
  • 免疫原氨基酸序列35-129位于human物种的COX5b的C-terminus
  • COX5b 抗体 (C-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat, human 来源的 COX5b
  • 抗COX5b抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取COX5b (C-5): sc-374416的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是COX5b Antibody (C-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与COX5b Antibody (C-5) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Cytochrome c Oxidase抗体，包括COX15, COX17, COX2, COX4, COX4I2, COX4NB, COX5a, COX5b, COX6b1和COX6c

COX5b抗体（C-5）是一种IgG1 κ小鼠单克隆COX5b抗体（也称为COX5B抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人的COX5b蛋白。COX5b抗体（C-5）既可以是非偶联的抗COX5b抗体形式，也可以是多种偶联形式的抗COX5b抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞色素c氧化酶（COX）家族蛋白作为呼吸链中的最终电子供体，驱动质子梯度穿过线粒体内膜，最终导致水的产生。哺乳动物的COX脱辅基酶是二聚体，每个单体由13个亚基组成，其中一些是线粒体的，一些是核的。COX5位于线粒体内膜上，是氧化酶家族中含血红素A的链，可将一分子氧和四分子氢转化为两分子水。COX5存在两种同工型，即COX5a和COX5b。当细胞内氧水平较高时，随着细胞呼吸速率的增加，COX5a（需氧同工型）的转录被上调。相反，当氧水平较低时，COX5b（缺氧同工型）的转录增加，并发挥作用使COX脱辅基酶的周转率最大化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COX5b 抗体 (C-5) 参考文献:

  1. 从常氧过渡到缺氧对酵母细胞色素 c 氧化酶和线粒体呼吸链的影响：缺氧基因诱导的意义。  |  David, PS. and Poyton, RO. 2005. Biochim Biophys Acta. 1709: 169-80. PMID: 16084486
  2. 鉴定皮肤鳞状细胞癌中的失调基因。  |  Dang, C., et al. 2006. Oncol Rep. 16: 513-9. PMID: 16865251
  3. 拟南芥中微核糖核酸对铜平衡的调控  |  Yamasaki, H., et al. 2007. J Biol Chem. 282: 16369-78. PMID: 17405879
  4. HIF-1 可调节细胞色素氧化酶亚基，优化缺氧细胞的呼吸效率。  |  Fukuda, R., et al. 2007. Cell. 129: 111-22. PMID: 17418790
  5. 细针穿刺结合实时 PCR：研究骨骼肌适应性功能变化的无痛方法。  |  Guescini, M., et al. 2007. Nutr Metab Cardiovasc Dis. 17: 383-93. PMID: 17482439
  6. 氧气和血红素对酵母 COX5 基因的反向调节。  |  Hodge, MR., et al. 1989. Mol Cell Biol. 9: 1958-64. PMID: 2546055
  7. 酵母细胞色素 c 氧化酶亚基 V 的异构体会影响双核反应中心，并改变与酵母细胞色素 c 异构体相互作用的动力学。  |  Allen, LA., et al. 1995. J Biol Chem. 270: 110-8. PMID: 7814361
  8. 人类细胞色素 c 氧化酶亚基 VB 启动子的系统发育足迹。  |  Bachman, NJ., et al. 1996. Arch Biochem Biophys. 333: 152-62. PMID: 8806766
  9. 细胞色素 c 氧化酶中氧调节同工酶的结构/功能。  |  Burke, PV. and Poyton, RO. 1998. J Exp Biol. 201: 1163-75. PMID: 9510528

订购信息

产品名称产品编号规格价格数量收藏夹

COX5b 抗体 (C-5)

sc-374416
200 µg/ml
RMB2377.00

COX5b (C-5): m-IgG Fc BP-HRP 套装

sc-529739
200 µg Ab; 10 µg BP
RMB2662.00

COX5b (C-5): m-IgGκ BP-HRP 套装

sc-522731
200 µg Ab, 40 µg BP
RMB2662.00

COX5b 抗体 (C-5) AC

sc-374416 AC
500 µg/ml, 25% agarose
RMB3129.00

COX5b 抗体 (C-5) HRP

sc-374416 HRP
200 µg/ml
RMB2377.00

COX5b 抗体 (C-5) FITC

sc-374416 FITC
200 µg/ml
RMB2482.00

COX5b 抗体 (C-5) PE

sc-374416 PE
200 µg/ml
RMB2580.00

COX5b 抗体 (C-5) Alexa Fluor® 488

sc-374416 AF488
200 µg/ml
RMB2685.00

COX5b 抗体 (C-5) Alexa Fluor® 546

sc-374416 AF546
200 µg/ml
RMB2685.00

COX5b 抗体 (C-5) Alexa Fluor® 594

sc-374416 AF594
200 µg/ml
RMB2685.00

COX5b 抗体 (C-5) Alexa Fluor® 647

sc-374416 AF647
200 µg/ml
RMB2685.00

COX5b 抗体 (C-5) Alexa Fluor® 680

sc-374416 AF680
200 µg/ml
RMB2685.00

COX5b 抗体 (C-5) Alexa Fluor® 790

sc-374416 AF790
200 µg/ml
RMB2685.00

Can COX5b (C-5): sc-374416 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, COX5b (C-5): sc-374416 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from excellent antibodyi got very strong bands. i used it i wb in dilution 1:250. i got this result in mouse tumor tissues.
Date published: 2018-05-11
Rated 3 out of 5 by from so much bandsGot the sample and tried it out. The results shows many band but also bands at the right position.
Date published: 2017-12-21
Rated 5 out of 5 by from Excellent antibody for WBThis antibody works very well in WB. A strong and clean signal at the predicted M.W on the WB (1:1000 dilution, Lung cancer cell line).
Date published: 2017-08-09
Rated 5 out of 5 by from Great mitochondrial marker antibody for IFAttached is an image of HeLa cells stained with this mAb (red) and the now discontinued polyclonal antibody against Tom20 (green).
Date published: 2017-06-27
Rated 1 out of 5 by from COX5b antibody does not work well for western blotThis antibody does not a give a strong signal in western blotting of mitochondrial enriched fractions
Date published: 2017-06-27
Rated 5 out of 5 by from WBright size and clear band has been detected in human lysate by WB.
Date published: 2017-04-19
Rated 5 out of 5 by from Large amount of positive signal in WB inLarge amount of positive signal in WB in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lung cancer tissue. -SCBT QC
Date published: 2015-03-24
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COX5b 抗体 (C-5) is rated 4.3 out of 5 by 9.
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