Date published: 2025-9-7

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Coronin 1C 抗体 (D-9): sc-376919

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说明书
  • Coronin 1C 抗体 D-9 是小鼠单克隆 IgM κ,Coronin 1C抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列390-474位于human物种的Coronin 1C的C-terminus
  • 推荐用于 mouse, rat 和 human 来源的Coronin 1C WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Coronin 1C (D-9): sc-376919免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Coronin 1C 抗体 (D-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Coronin 1C抗体(D-9)是一种IgM κ小鼠单克隆Coronin 1C抗体(也被称作CORO1C抗体或Coronin 3抗体),它通过WB、IP、IF、IHC(P)和ELISA检测方法检测小鼠、大鼠和人类来源的Coronin 1C蛋白。Coronin 1C抗体(D-9)可以作为非偶联形式的抗Coronin 1C抗体使用。Coronins是一类包含WD重复序列的、与肌动蛋白结合的蛋白质家族,它们定位于亚膜区域,并调节细胞运动和细胞骨架重排。Coronin 1A(CORO1A、CLIPINA、CLABP、TACO、p57)可以形成卷曲螺旋介导的同源三聚体复合物,影响早期吞噬体的形成。Coronin 1B(CORO1B)在丝氨酸2处的PKC依赖性磷酸化通过与Arp2/3复合物的相互作用调节成纤维细胞的前沿动态和细胞运动。Coronin 1C(CORO1C、Coronin 3、HCRNN4)在分化的Neuro-2a细胞、PC-12细胞和原代少突胶质细胞中丰富,在这些细胞中,它被认为会影响神经元的形态发生和迁移。Coronin 2A(CORO2A、CLIPINB、IR10、WDR2)是大约1.5-2百万道尔顿的N-CoR(核受体共抑制因子)复合物(由10-12个蛋白质组成)的组成部分,该复合物招募HDACs以生成抑制性染色质。Coronin 7(CORO7、CRN7)定位于高尔基体膜,并影响细胞内膜隔室和囊泡运输的组织。Coronin 2B(CORO2B、CLIPINC)和Coronin 6(CORO6)与此家族的其他成员相似,因为它们具有保守的基本N端基序和聚集在一个或两个核心结构域中的3-10个WD重复序列。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Coronin 1C 抗体 (D-9) 参考文献:

  1. Coronin 定域于前缘,参与脊椎动物细胞的细胞扩散和薄层基质扩展。  |  Mishima, M. and Nishida, E. 1999. J Cell Sci. 112 (Pt 17): 2833-42. PMID: 10444378
  2. 无处不在的哺乳动物冠状病毒 3 的聚合, F-肌动蛋白相互作用和膜缔合作用是由其羧基末端介导的。  |  Spoerl, Z., et al. 2002. J Biol Chem. 277: 48858-67. PMID: 12377779
  3. 人类 N-CoR 复合物的纯化和功能特征:HDAC3, TBL1 和 TBLR1 的作用。  |  Yoon, HG., et al. 2003. EMBO J. 22: 1336-46. PMID: 12628926
  4. 哺乳动物 POD-1 的同源物 Coronin 7 定位于高尔基体。  |  Rybakin, V., et al. 2004. FEBS Lett. 573: 161-7. PMID: 15327992
  5. 白细胞质膜通过盘绕线圈介导的三聚冠状蛋白 1 分子与肌动蛋白细胞骨架缔合作用。  |  Gatfield, J., et al. 2005. Mol Biol Cell. 16: 2786-98. PMID: 15800061
  6. 冠状病毒素 3 及其在小鼠大脑形态发生中的作用  |  Hasse, A., et al. 2005. Eur J Neurosci. 21: 1155-68. PMID: 15813925
  7. 吞噬体的形成需要 Coronin-1 的功能。  |  Yan, M., et al. 2005. Mol Biol Cell. 16: 3077-87. PMID: 15829569
  8. Coronin 蛋白是细胞骨架和膜贩运的多功能调节器。  |  Rybakin, V. and Clemen, CS. 2005. Bioessays. 27: 625-32. PMID: 15892111
  9. 蛋白激酶 C 对冠状病毒素 1B 的磷酸化调节其与 Arp2/3 的相互作用和细胞运动。  |  Cai, L., et al. 2005. J Biol Chem. 280: 31913-23. PMID: 16027158
  10. Coronin-1C是一种新型肝细胞癌侵袭性进展生物标志物,通过蛋白质组学分析和临床验证确定。  |  Wu, L., et al. 2010. J Exp Clin Cancer Res. 29: 17. PMID: 20181269
  11. 冠蛋白1C通过调节MT1-MMP的运输和侵袭足的功能,促进三阴性乳腺癌的侵袭性。  |  Castagnino, A., et al. 2018. Oncogene. 37: 6425-6441. PMID: 30065298
  12. 冠蛋白1C受多种microRNA调节,促进胰腺导管腺癌癌细胞的侵袭性。  |  Fukuda, K., et al. 2023. Genes (Basel). 14: PMID: 37239355

订购信息

产品名称产品编号规格价格数量收藏夹

Coronin 1C 抗体 (D-9)

sc-376919
200 µg/ml
RMB2377.00

I would like to ask whether this antibody has been test for Immunofluorescence and validated on knock-down/knock-out cell lines.

Asked by: vidot93
Thank you for your question. Coronin 1C Antibody (D-9): sc-376919 has been tested in a variety of different assays ans species. It is recommended for detection of Coronin 1C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, so it is suitable for immunofluorescence experiments.
Answered by: Tech Support Europe
Date published: 2020-07-15

I am using Coronin 1C (D-9): sc-376919 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good for WBGood signal for western blot analysis (CRC cell lines)
Date published: 2022-09-06
Rated 5 out of 5 by from Great Ab for WBUsed a 1:300 dilution for WB with 1- SUM159, 2- Caco-2, and 3- 293-T cell lysates. Detected a clean, strong band.
Date published: 2018-01-30
Rated 4 out of 5 by from WB for mouse brain was workedWB for mouse brain was worked. Signal intensity was normal.
Date published: 2017-12-09
Rated 1 out of 5 by from NOT WORKINGI used this antibody for WB. It seems like it's not working at all. I used 1:1000, 1:500, 1:100 there is no bands although I exposed using strong ECL. I used Hela, HEK293 and MDA-MB-231 which known to express coronin 1c, but there is nothing... this ab basically not working at all for WB
Date published: 2017-08-14
Rated 5 out of 5 by from Produced great immunoperoxidase membraneProduced great immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-02-14
Rated 5 out of 5 by from Produced positive Western Blot data of CoroninProduced positive Western Blot data of Coronin 1C expression in HeLa nuclear extract and T24 whole cell lysate . -SCBT QC
Date published: 2014-08-23
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Coronin 1C 抗体 (D-9) is rated 4.0 out of 5 by 6.
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