Date published: 2025-9-6
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COL7A1 Antibody (4D2) - Immunofluorescence - Image 12158
COL7A1 抗体 (4D2): sc-33710. 正常人角膜冰冻切片的免疫荧光染色, 显示基底膜染色.
COL7A1 Antibody (4D2) - Immunofluorescence - Image 12158
COL7A1 抗体 (4D2): sc-33710. 正常人角膜冰冻切片的免疫荧光染色, 显示基底膜染色.
COL7A1 Antibody (4D2) - Immunohistochemistry - Image 37597
COL7A1 抗体 (4D2): sc-33710. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色, 低倍 (A) 和高倍 (B) 放大后显示肾小球和肾小管细胞的胞浆染色. 瑞典人类蛋白质图谱 (HPA) 计划友情提供.
COL7A1 抗体 (4D2): sc-33710. 正常人角膜冰冻切片的免疫荧光染色, 显示基底膜染色.

COL7A1 抗体 (4D2): sc-33710

5.0(5)
寫評論提問

说明书
  • COL7A1 抗体 4D2 是小鼠单克隆 IgG1 κ，COL7A1抗体, 在23篇文献中引用，规格为200 µg/ml
  • 针对正常human皮肤SDS不溶性蛋白
  • COL7A1 抗体 (4D2) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 Collagen Type VII
  • 抗COL7A1抗体（4D2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取COL7A1 (4D2): sc-33710的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 COL7A1 抗体 (4D2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与COL7A1 抗体 (4D2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Collagen 抗体 包括 COL10A1, COL12A1, COL14A1, COL17A1, COL18A1, COL1A, COL1A1, COL1A2, COL23A1和COL2A1

COL7A1抗体（4D2）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测人源COL7A1蛋白。抗COL7A1抗体（4D2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。COL7A1，也称为VII型胶原蛋白，在形成连接表皮和真皮的锚定纤维方面起着至关重要的作用，为皮肤提供结构完整性。COL7A1主要位于基底膜区，在那里COL7A1对于维持皮肤的稳定性和抗机械应力的弹性至关重要。COL7A1基因突变可导致营养不良性表皮松解症，这是一种严重的皮肤水泡性疾病，凸显了COL7A1在皮肤健康和疾病中的重要性。了解COL7A1的功能和定位对于制定与COL7A1功能障碍相关的疾病的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COL7A1 抗体 (4D2) 参考文献:

  1. 胶原蛋白和骨骼强度  |  Boskey, AL., et al. 1999. J Bone Miner Res. 14: 330-5. PMID: 10027897
  2. 评论文章：血小板与胶原蛋白的相互作用：膜受体和细胞内信号途径。  |  Alberio, L. and Dale, GL. 1999. Eur J Clin Invest. 29: 1066-76. PMID: 10583456
  3. ABE8e腺嘌呤碱基编辑器精确高效地纠正了COL7A1的反复出现的无义突变。  |  Sheriff, A., et al. 2022. Sci Rep. 12: 19643. PMID: 36385635
  4. 通过 RDEB 衍生皮肤等效物中的 RNA 转接剪接编辑 COL7A1。  |  Liemberger, B., et al. 2023. Int J Mol Sci. 24: PMID: 36901775
  5. 胞嘧啶脱氨酶碱基编辑可恢复营养不良性表皮松解症人类： 小鼠皮肤模型。  |  Naso, G., et al. 2023. JID Innov. 3: 100191. PMID: 37213713
  6. COL7A1表达可改善透明细胞肾细胞癌患者分期预测。  |  Koca, D., et al. 2023. Cancers (Basel). 15: PMID: 37345040
  7. 通过逐步分馏，高支化聚（β-氨基酯）介导的难以转染的皮肤细胞中COL7A1和TGM1基因表达增强。  |  Pan, C., et al. 2024. J Control Release. 370: 82-94. PMID: 38643938
  8. COL7A1/PI3K/AKT轴调控胆管癌的进展。  |  Ma, Y., et al. 2024. Heliyon. 10: e37361. PMID: 39309925
  9. 细胞粘附到胶原基质上。  |  McCarthy, JB., et al. 1996. Biopolymers. 40: 371-81. PMID: 8765608
  10. 无脊椎动物的多功能胶原。  |  Engel, J. 1997. Science. 277: 1785-6. PMID: 9324767
  11. 软骨胶原：综述其结构, 组织以及在动物实验性关节炎和人类风湿病发病机制中的作用。  |  Cremer, MA., et al. 1998. J Mol Med (Berl). 76: 275-88. PMID: 9535561

订购信息

产品名称产品编号规格价格数量收藏夹

COL7A1 抗体 (4D2)

sc-33710
200 µg/ml
RMB2377.00

COL7A1 (4D2): m-IgG Fc BP-HRP 套装

sc-526709
200 µg Ab; 10 µg BP
RMB2662.00

COL7A1 (4D2): m-IgG1 BP-HRP 套装

sc-532082
200 µg Ab; 20 µg BP
RMB2662.00

COL7A1 抗体 (4D2) AC

sc-33710 AC
500 µg/ml, 25% agarose
RMB3129.00

COL7A1 抗体 (4D2) HRP

sc-33710 HRP
200 µg/ml
RMB2377.00

COL7A1 抗体 (4D2) FITC

sc-33710 FITC
200 µg/ml
RMB2482.00

COL7A1 抗体 (4D2) PE

sc-33710 PE
200 µg/ml
RMB2580.00

COL7A1 抗体 (4D2) Alexa Fluor® 488

sc-33710 AF488
200 µg/ml
RMB2685.00

COL7A1 抗体 (4D2) Alexa Fluor® 546

sc-33710 AF546
200 µg/ml
RMB2685.00

COL7A1 抗体 (4D2) Alexa Fluor® 594

sc-33710 AF594
200 µg/ml
RMB2685.00

COL7A1 抗体 (4D2) Alexa Fluor® 647

sc-33710 AF647
200 µg/ml
RMB2685.00

COL7A1 抗体 (4D2) Alexa Fluor® 680

sc-33710 AF680
200 µg/ml
RMB2685.00

COL7A1 抗体 (4D2) Alexa Fluor® 790

sc-33710 AF790
200 µg/ml
RMB2685.00

Has the exact binding site of this antibody within collagen 7 been characterized yet?

Asked by: Josy123
Thank you for your question. No epitope mapping has been performed for this antibody as of yet. Its exact binding site is not known at this point.
Answered by: Tech Support Europe
Date published: 2022-02-05

Do you know if this antibody (COL7A1 Antibody (4D2): sc-33710) reacts with mouse, or only human? We are searching for an anti-COL7A1 antibody that will NOT recognized mouse collagen VII, only human, and is conjugated. Thanks for the help.

Asked by: Dorothy
Thank you for your question. We recommend sc-33710:COL7A1 (4D2) for use with human samples. The exact binding site of sc-33710:COL7A1 (4D2) has not been characterized and, therefore, unable to determine homolgy with mouse COL7A1. We offer sc-33710:COL7A1 (4D2) conjugated to HRP and a variety of fluorochromes. If you are interested, we are providing complimentary samples of the unconjugated version of this antibody for a limited time. Please contact technical service for further information.
Answered by: Santa Cruz TS
Date published: 2017-05-19

Can COL7A1 (4D2): sc-33710 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, COL7A1 (4D2): sc-33710 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from strong staining in human skinWe use this antibody to mark the basement membrane in human skin cryosections. The antibody works very well and we consistenly get great IF images.
Date published: 2022-03-21
Rated 5 out of 5 by from Strong IF stainingWe used this antibody for IF staining and were able to produce very sharp images showing membrane localization
Date published: 2017-01-25
Rated 5 out of 5 by from IHC on paraffin embedded sections of humanIHC on paraffin embedded sections of human skin (dilution 1/50) - Published data
Date published: 2015-08-07
Rated 5 out of 5 by from Antibody works great for IHC paraffinAntibody works great for IHC paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-20
Rated 5 out of 5 by from Worked well for immunofluorescence stainingWorked well for immunofluorescence staining of normal human cornea frozen section. -SCBT QC
Date published: 2015-01-11
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COL7A1 抗体 (4D2) is rated 5.0 out of 5 by 5.
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