Date published: 2025-9-6
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COL3A1 Antibody (B-10) - Western Blotting - Image 375700
COL3A1 抗体 (B-10): sc-271249. C3H/10T1/2 全细胞裂解液中 COL3A1 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂进行阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
COL3A1 抗体 (B-10): sc-271249. C3H/10T1/2 全细胞裂解液中 COL3A1 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂进行阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

COL3A1 抗体 (B-10): sc-271249

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寫評論提問

说明书
  • COL3A1 抗体 B-10 是小鼠单克隆 IgG1 κ，COL3A1抗体, 在82篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列901-1200位于human物种的Collagen α1 Type III的C-terminus附近
  • COL3A1 抗体 (B-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Collagen α1 Type III
  • 抗COL3A1抗体（B-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取COL3A1 (B-10): sc-271249的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是COL3A1 Antibody (B-10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与COL3A1 Antibody (B-10) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Collagen抗体，包括COL10A1, COL12A1, COL14A1, COL17A1, COL18A1, COL1A, COL1A1, COL1A2, COL23A1, COL2A1, COL3A1, COL4A, COL4A1, COL4A6, COL5A1和COL5A2

COL3A1抗体（B-10）是一种IgG1 κ小鼠单克隆COL3A1抗体（也称为III型胶原蛋白α1链抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的COL3A1蛋白。COL3A1抗体（B-10）既可以是非偶联的抗COL3A1抗体形式，也可以是多种偶联形式的抗COL3A1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。COL基因产物（胶原蛋白）的广泛家族由几种链类型组成，包括纤维形成间质胶原蛋白（I型、II型、III型和V型）和基底膜胶原蛋白（IV型），每种类型包含多种异构体。胶原蛋白是具有高拉伸强度的纤维状细胞外基质蛋白，是结缔组织的主要成分，如肌腱和软骨。所有胶原蛋白都含有一个三螺旋结构域，并经常显示横向自我关联，以形成复杂的结缔组织。几种胶原蛋白还在细胞粘附中发挥作用，对维持正常组织结构和功能至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COL3A1 抗体 (B-10) 参考文献:

  1. 胶原蛋白和骨骼强度  |  Boskey, AL., et al. 1999. J Bone Miner Res. 14: 330-5. PMID: 10027897
  2. 评论文章：血小板与胶原蛋白的相互作用：膜受体和细胞内信号途径。  |  Alberio, L. and Dale, GL. 1999. Eur J Clin Invest. 29: 1066-76. PMID: 10583456
  3. COL3A1基因的变异类型与血管性埃勒斯-当洛斯综合征的表型和严重程度有关。  |  Frank, M., et al. 2015. Eur J Hum Genet. 23: 1657-64. PMID: 25758994
  4. III型胶原蛋白（COL3A1）： 基因和蛋白质结构、组织分布及相关疾病。  |  Kuivaniemi, H. and Tromp, G. 2019. Gene. 707: 151-171. PMID: 31075413
  5. COL3A1/COL5A2缺失患者的表型。  |  Kempers, MJ., et al. 2022. Eur J Med Genet. 65: 104593. PMID: 35964930
  6. COL3A1过表达与肺癌的不良预后和顺铂耐药性有关。  |  Wang, L., et al. 2022. Balkan Med J. 39: 393-400. PMID: 36148899
  7. 对大量和单个细胞测序数据的综合分析显示，EMP1+/COL3A1+成纤维细胞可促进乳腺癌、前列腺癌和肾癌的骨转移过程。  |  Du, H., et al. 2023. Front Immunol. 14: 1313536. PMID: 38187400
  8. COL3A1在调节顺铂耐药性非小细胞肺癌细胞的生长、转移和药物敏感性方面的作用和机制。  |  Ren, J., et al. 2024. Cancer Biol Ther. 25: 2328382. PMID: 38530094
  9. 细胞粘附到胶原基质上。  |  McCarthy, JB., et al. 1996. Biopolymers. 40: 371-81. PMID: 8765608
  10. 无脊椎动物的多功能胶原。  |  Engel, J. 1997. Science. 277: 1785-6. PMID: 9324767
  11. 软骨胶原：综述其结构, 组织以及在动物实验性关节炎和人类风湿病发病机制中的作用。  |  Cremer, MA., et al. 1998. J Mol Med (Berl). 76: 275-88. PMID: 9535561

订购信息

产品名称产品编号规格价格数量收藏夹

COL3A1 抗体 (B-10)

sc-271249
200 µg/ml
RMB2377.00

COL3A1 (B-10): m-IgGκ BP-HRP 套装

sc-521891
200 µg Ab, 40 µg BP
RMB2662.00

COL3A1 抗体 (B-10) AC

sc-271249 AC
500 µg/ml, 25% agarose
RMB3129.00

COL3A1 抗体 (B-10) HRP

sc-271249 HRP
200 µg/ml
RMB2377.00

COL3A1 抗体 (B-10) FITC

sc-271249 FITC
200 µg/ml
RMB2482.00

COL3A1 抗体 (B-10) PE

sc-271249 PE
200 µg/ml
RMB2580.00

COL3A1 抗体 (B-10) Alexa Fluor® 488

sc-271249 AF488
200 µg/ml
RMB2685.00

COL3A1 抗体 (B-10) Alexa Fluor® 546

sc-271249 AF546
200 µg/ml
RMB2685.00

COL3A1 抗体 (B-10) Alexa Fluor® 594

sc-271249 AF594
200 µg/ml
RMB2685.00

COL3A1 抗体 (B-10) Alexa Fluor® 647

sc-271249 AF647
200 µg/ml
RMB2685.00

COL3A1 抗体 (B-10) Alexa Fluor® 680

sc-271249 AF680
200 µg/ml
RMB2685.00

COL3A1 抗体 (B-10) Alexa Fluor® 790

sc-271249 AF790
200 µg/ml
RMB2685.00

Hi! Sorry for bothering you. Using COL3A1 Antibody (B-10): sc-271249 I have detected Collagen type 3 in cancer cells with molecular weight around 70kDa. Usually, Col3a1 is around 100 or higher. Can you please comment on this? Thank you.

Asked by: fdds
Thank you for your questions. For further assistance please call or email technical service. (800)-457-3801 or [email protected]
Answered by: BlakeJ
Date published: 2023-03-16

What is the dilution used for immunohistochemistry? Thank you

Asked by: marcelnani
Thank you for your question. We recommend a starting dilution of 1:50 for IHC applications with this antibody.
Answered by: Tech Service
Date published: 2019-12-03

Does your antibody recognize nature or denature form of human collagen III, under reducing or nonreducing condition?

Asked by: SU33
Thank you for your question. All of the published Western blot data is using this antibody under reducing and denaturing conditions. We have not tested this antibody under non-reducing conditions.
Answered by: Technical Support
Date published: 2019-04-13

I tested the COL3A1 Antibody (B-10): sc-271249 in human fibroblasts. I got a clear band but it was close to 250kD. And I found some other companies' COL3A1 antibodies also recognize a 250kD band. Does anyone have the observation?

Asked by: Zhijie
Thank you for your question. We have representative Western blot data using this specific antibody published on our website. We would recommend searching the primary literature for further information about higher molecular weight forms of COL3A1 that might be observed in Western blot.
Answered by: Technical Support
Date published: 2018-11-19

I am studying COL3A1, but not in human samples. Will this antibody work in other species?

Asked by: cjMara
COL3A1 (B-10): sc-271249 monoclonal antibody is recommended for detection of Collagen α1 Type III of mouse, rat and human origin. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-01-30
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Rated 3 out of 5 by from 条带显示不好WB试验，上样量40ug，多次试验结果，没有明显条带出现，不知道小样的品质与正品有没有差别，看图还是有点伤心的
Date published: 2018-10-11
Rated 5 out of 5 by from Strong bands in western blotStrong bands on human dermal fibroblast cell lysate. Primary was incubated at 1:200 for 1 hour at RT. 5% milk was used as a block. Proteins were transferred on nitrocellulose.
Date published: 2018-04-02
Rated 4 out of 5 by from Col3a1Band at the expected size >100kDa but also a smaller one around 30kDa.
Date published: 2017-12-21
Rated 5 out of 5 by from great for cardio fibroblastsIt is great for WB and staining on cardio fibroblasts.
Date published: 2017-12-14
Rated 5 out of 5 by from Works really well!We used it 1:1000 diluted in 1% milk T-TBS in a western blot (9% Laemmli) for heart lissates. The bands were strong and clear and the molecular weight was about 100kDa. The antibody resulted specific and worked really well.
Date published: 2017-10-26
Rated 5 out of 5 by from ExcellentWe have used this antibody on human skin tissue. It works great on both western blot and IHC. It stains papillae matrix in upper dermis very well.
Date published: 2017-09-20
Rated 5 out of 5 by from good antibodyThis antibody is so good for western blot, it shows a clear band
Date published: 2017-07-25
Rated 5 out of 5 by from COL3A1 (B-10) antibodyI have done the Western blot, the band is clear and good !
Date published: 2017-05-08
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COL3A1 抗体 (B-10) is rated 4.6 out of 5 by 16.
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