Date published: 2025-9-6
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COL2A1 Antibody (M2139) - Western Blotting - Image 14010
COL2A1 (M2193): sc-52658. CCD-1064Sk 全细胞裂解液中 COL2A1 表达的 Western 印迹分析.
COL2A1 Antibody (M2139) - Western Blotting - Image 14010
COL2A1 (M2193): sc-52658. CCD-1064Sk 全细胞裂解液中 COL2A1 表达的 Western 印迹分析.
COL2A1 Antibody (M2139) - Western Blotting - Image 137874
COL2A1 抗体 (M2139): sc-52658. HISM (A) 和 CCD-1064Sk (B) 全细胞裂解液中 COL2A1 表达的 Western 印迹分析.
COL2A1 Antibody (M2139) - Western Blotting - Image 51726
COL2A1 抗体 (M2139): sc-52658. 对 HISM 全细胞裂解液中 COL2A1 的表达进行 Western 印迹分析.
COL2A1 (M2193): sc-52658. CCD-1064Sk 全细胞裂解液中 COL2A1 表达的 Western 印迹分析.

COL2A1 抗体 (M2139): sc-52658

4.8(17)
寫評論提問

说明书
  • COL2A1 抗体 M2139 是小鼠单克隆 IgG2b κ，COL2A1抗体, 在172篇文献中引用，规格为100 µg/ml
  • 针对mouse起源的胶原蛋白II J1表位（三重螺旋位置551-564）
  • 推荐用于 mouse, rat， human 和 Arabadopsis 来源的Collagen Type II WB, IP, IF 和 IHC(P)检测; 也和以下物种反应，包括： bovine
  • 关于如何获取COL2A1 (M2139): sc-52658的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 COL2A1 抗体 (M2139) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与COL2A1 抗体 (M2139) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Collagen 抗体 包括 COL10A1, COL12A1, COL14A1, COL17A1, COL18A1, COL1A, COL1A1, COL1A2, COL23A1和COL2A1

COL2A1 抗体 (M2139) 是一种 IgG2b κ 小鼠单克隆 COL2A1 抗体（也称为胶原 II 型 alpha 1 链抗体），可通过 WB、IP、IF 和 IHC(P) 检测小鼠、大鼠、人类和拟南芥的胶原 II 型蛋白。COL2A1 抗体（M2139）为非结合型抗 COL2A1 抗体。广泛的 COL 基因产物（胶原）家族由几种链类型组成，包括纤维形成的间质胶原（I、II、III 和 V 型）和基底膜胶原（IV 型），每种类型都包含多种同工酶。胶原是纤维状细胞外基质蛋白，具有很高的抗张强度，是肌腱和软骨等结缔组织的主要成分。所有胶原蛋白都含有一个三重螺旋结构域，经常出现横向自结合，以形成复杂的结缔组织。几种胶原还在细胞粘附中发挥作用，对维持正常的组织结构和功能非常重要。在软骨中，Ⅱ型胶原蛋白是纤维的主体。用 II 型胶原蛋白致敏会诱发大鼠、小鼠和高等灵长类动物的侵蚀性多关节炎，这种关节炎类似于类风湿性关节炎和复发性多软骨炎。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COL2A1 抗体 (M2139) 参考文献:

  1. Col2a1系谱追踪显示膝关节半月板的细胞起源复杂。  |  Hyde, G., et al. 2008. J Anat. 213: 531-8. PMID: 19014360
  2. COL2A1相关的脊椎骨骺发育不良的骨骼退化发生在骨关节炎之前。  |  Rolvien, T., et al. 2020. Osteoarthritis Cartilage. 28: 334-343. PMID: 31958497
  3. COL2A1的新变体可导致罕见的先天性脊柱骨骺发育不良。  |  Zheng, WB., et al. 2020. Mol Genet Genomic Med. 8: e1139. PMID: 31972903
  4. 一个早发高度近视/单眼Stickler综合征家族中的新型深内含子COL2A1突变。  |  Sun, W., et al. 2020. Ophthalmic Physiol Opt. 40: 281-288. PMID: 32196734
  5. COL2A1是黑色素瘤肿瘤再填充细胞的新生物标志物。  |  Talluri, B., et al. 2020. Biomedicines. 8: PMID: 32962144
  6. 外显子组测序显示，在一个患有多种骨骺发育不良症的大家族中，USP9X和COL2A1发生了突变。  |  Luo, ZJ., et al. 2022. Bone. 163: 116508. PMID: 35907616
  7. COL2A1剪接变体的分子复杂性及其在表型严重程度中的意义。  |  Viakhireva, I., et al. 2024. Bone. 181: 117013. PMID: 38246255
  8. 细胞粘附到胶原基质上。  |  McCarthy, JB., et al. 1996. Biopolymers. 40: 371-81. PMID: 8765608
  9. 鼻腔对 II 型胶原蛋白显性和亚显性表位的耐受性以及对胶原蛋白诱发的关节炎的保护作用。  |  Staines, NA., et al. 1997. Biochem Soc Trans. 25: 661-4. PMID: 9191176
  10. 无脊椎动物的多功能胶原。  |  Engel, J. 1997. Science. 277: 1785-6. PMID: 9324767
  11. 胶原蛋白诱发的关节炎，一种自身免疫的动物模型。  |  Myers, LK., et al. 1997. Life Sci. 61: 1861-78. PMID: 9364191
  12. 软骨胶原：综述其结构, 组织以及在动物实验性关节炎和人类风湿病发病机制中的作用。  |  Cremer, MA., et al. 1998. J Mol Med (Berl). 76: 275-88. PMID: 9535561

订购信息

产品名称产品编号规格价格数量收藏夹

COL2A1 抗体 (M2139)

sc-52658
100 µg/ml
RMB2377.00

COL2A1 (M2139): m-IgG Fc BP-HRP 套装

sc-538958
100 µg Ab; 10 µg BP
RMB2662.00

COL2A1 (M2139): m-IgG2b BP-HRP 套装

sc-549724
100 µg Ab; 10 µg BP
RMB2662.00

对兔子来源的细胞具有亲和性吗？

Asked by: toofoog
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-12-02

I would like to detection of collagen type 2 in western blot assay. I tried dilution 1:500 in 5% skinmilk. and 2nd antibody used sc-2005 product. but It does not detection. How can I do? my sample orign is human pellet.

Asked by: vtml
Thank you for your question. We recommend starting with a 1:100 primary antibody dilution for Western blot. For further assistance, please contact Technical Service.
Answered by: Technical Support
Date published: 2019-01-18

Is trial pack available for this antibody ?

Asked by: Ghosh
Thank you for the question. COL2A1 Antibody (M2139) is a mouse monoclonal mouse IgG2b κ (kappa light chain) provided at 100 µg/ml. We currently do offer Sample sizes for this product. Please request this with your supplier. However, you can also purchase the product with our 1 year full product warranty if you are planning to use this product in any of the warranted applications and warranted species: recommended for detection of Collagen Type II of mouse, rat, human and a origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine.
Answered by: Technical Support Europe
Date published: 2017-12-03

I would like to do some IF on my mouse samples. Do you have any suggestions on how this can be successful using a mouse monoclonal primary?

Asked by: Cweed
We recommend using a a directly conjugated primary antibodies. Please contact our special orders department for pricing and availability of sc-52658 conjugates.
Answered by: Technical Support 10
Date published: 2017-01-12

I can find the donkey anti-mouse secondary that I am accustomed to using. What do you recommend as a replacement?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-30
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Rated 1 out of 5 by from ATDC5では検出できずATDC5はマウス由来の前駆軟骨細胞株としてしられています。今回定法に従い分化誘導をかけ、回収した細胞に対してM2139を試用しました。遺伝子の発現誘導はPCRで検出されましたが、目的サイズにバンドは検出されませんでした。原因の一つとしては二次抗体が推奨のものでは無かったことが考えられます。
Date published: 2025-04-08
Rated 5 out of 5 by from good antibodyworked well on mouse FFPE sections 1:100 dilution Antigen retrieval: Proteinase K (20ug/mL), 37°C for 20 minutes
Date published: 2024-02-12
Rated 5 out of 5 by from well function in WB, IF and IHCGood results when tested COL2A1 in western blot of whole cell extracts or IF, IHC from rat articular chondrocytes.
Date published: 2023-12-22
Rated 5 out of 5 by from Works well for WBIt works well for WB by the samples. And am trying IF recently.
Date published: 2023-10-18
Rated 4 out of 5 by from It works for flow cytometry and microscopy!While the antibody has some background compared with unstained cells at 1:50 (probably helped by the facts that it is an IgG2a antibody and not an IgG1 antibody and we worked with myeloid cells), it showed staining in microscopy (with DAPI as counterstain) and a shift in intensity by flow cytometry for target-expressing cells compared to non-expressing cells! We used goat anti-mouse A488 as secondary antibody. Happy we gave this antibody a try.
Date published: 2022-10-27
Rated 4 out of 5 by from buono in immuno e wbHo utilizzato questo anticorpo sia in immunofluorescenza (diluizione usata 1:100), che in wb (usato 1:1000) su midollo spinale di topo e sono rimasta molto soddisfatta del risultato ottenuto
Date published: 2020-10-06
Rated 4 out of 5 by from 凍結切片で免疫染色可能無料サンプルをご提供いただき、凍結切片の免疫染色に用いました。条件検討の結果、染色像が得られました。
Date published: 2020-09-16
Rated 5 out of 5 by from Good antibody for ICCGood antibody for ICC! Application: ICC Sample: rat chondrocytes
Date published: 2020-08-25
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COL2A1 抗体 (M2139) is rated 4.6 out of 5 by 18.
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