Date published: 2025-9-6
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COL1A Antibody (COL-1) - Immunofluorescence - Image 145258
COL1A 抗体 (COL-1): sc-59772. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜和细胞质定位.
COL1A Antibody (COL-1) - Immunofluorescence - Image 145258
COL1A 抗体 (COL-1): sc-59772. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜和细胞质定位.
COL1A 抗体 (COL-1): sc-59772. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜和细胞质定位.

COL1A 抗体 (COL-1): sc-59772

4.5(13)
寫評論提問

说明书
  • COL1A 抗体 COL-1 是小鼠单克隆 IgG1，COL1A抗体, 在222篇文献中引用，规格为100 µl ascites
  • 针对全长天然bovine I型胶原蛋白的抗体
  • 推荐用于通过WB（非还原）、IP、IF、IHC（冷冻）和ELISA检测native Collagen Type I、mouse, rat和human来源的native Collagen Type I; 也和以下物种反应，包括： 和 bovine, porcine, feline and canine; 可能与 connective tissue fibers in acetone-fixed or unfixed frozen sections产生交联反应; 不会与 Collagen Types II, III, IV, V, VI, VII, IX, X and XI产生交联反应
  • 目前，我们还没有完成COL1A 抗体 (COL-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案

関連項目

此外 Collagen 抗体 包括 COL10A1, COL12A1, COL14A1, COL17A1, COL18A1, COL1A, COL1A1, COL1A2, COL23A1和COL2A1

COL1A抗体（COL-1）是一种IgG1小鼠单克隆COL1A抗体（也称为I型胶原蛋白α1链抗体、I型胶原蛋白前α1链抗体、α-1 I型胶原蛋白抗体、软骨、骨骼、肌腱、皮肤相关结构蛋白抗体，或COL1A1基因产物特异性抗体），它可通过WB、IP、IF和ELISA技术检测小鼠、大鼠和人源的COL1A蛋白。COL1A抗体（COL-1）有非偶联抗COL1A抗体形式。COL基因产物（胶原蛋白）的大家族由多种链类型组成，包括形成纤维的间质胶原蛋白（I型、II型、III型和V型）和基底膜胶原蛋白（IV型），每种类型都包含多种异构体。胶原蛋白是具有高拉伸强度的纤维状细胞外基质蛋白，是结缔组织（如肌腱和软骨）的主要成分。所有的胶原蛋白都包含一个三螺旋结构域，并经常表现出横向自我关联，以形成复杂的结缔组织。一些胶原蛋白还在细胞粘附中发挥作用，这对于维持正常的组织结构和功能至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COL1A 抗体 (COL-1) 参考文献:

  1. 胶原蛋白和骨骼强度  |  Boskey, AL., et al. 1999. J Bone Miner Res. 14: 330-5. PMID: 10027897
  2. 评论文章：血小板与胶原蛋白的相互作用：膜受体和细胞内信号途径。  |  Alberio, L. and Dale, GL. 1999. Eur J Clin Invest. 29: 1066-76. PMID: 10583456
  3. 1044 名瑞典老年妇女的 1 型胶原蛋白 (COL1A 1) Sp1 结合位点多态性与股骨颈骨矿物质密度和腕部骨折的关系。  |  Gerdhem, P., et al. 2004. Calcif Tissue Int. 74: 264-9. PMID: 14595528
  4. 大鼠α1Ⅰ型胶原蛋白基因远端启动子区域的转化生长因子-β激活因子。  |  Ritzenthaler, JD., et al. 1991. Biochem J. 280 (Pt 1): 157-62. PMID: 1741743
  5. 甲状腺乳头状癌中α-1 I型胶原表达的预后潜力。  |  Huang, C., et al. 2019. Biochem Biophys Res Commun. 515: 125-132. PMID: 31128912
  6. Ⅰ型胶原蛋白α1链的预后预测、免疫微环境和耐药性价值：从胃肠道癌症到泛癌症分析。  |  Liu, Y., et al. 2021. Front Mol Biosci. 8: 692120. PMID: 34395525
  7. 成骨不全小鼠的Col1A-2突变通过改变细胞基质组织导致长骨脆弱。  |  André, G., et al. 2023. Int J Mol Sci. 24: PMID: 38069332
  8. 细胞粘附到胶原基质上。  |  McCarthy, JB., et al. 1996. Biopolymers. 40: 371-81. PMID: 8765608
  9. 鼻腔对 II 型胶原蛋白显性和亚显性表位的耐受性以及对胶原蛋白诱发的关节炎的保护作用。  |  Staines, NA., et al. 1997. Biochem Soc Trans. 25: 661-4. PMID: 9191176
  10. 无脊椎动物的多功能胶原。  |  Engel, J. 1997. Science. 277: 1785-6. PMID: 9324767
  11. 胶原蛋白诱发的关节炎，一种自身免疫的动物模型。  |  Myers, LK., et al. 1997. Life Sci. 61: 1861-78. PMID: 9364191
  12. 软骨胶原：综述其结构, 组织以及在动物实验性关节炎和人类风湿病发病机制中的作用。  |  Cremer, MA., et al. 1998. J Mol Med (Berl). 76: 275-88. PMID: 9535561

订购信息

产品名称产品编号规格价格数量收藏夹

COL1A 抗体 (COL-1)

sc-59772
100 µl ascites
$316.00

Hi, can you suggest a protocol of western blot for detecting this native collagen from porcine samples?

Asked by: danK
Thank you for your question. You can find our recommended western blot protocol under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2021-02-03

Hello, is there an SDS document for this product? Our Health & Safety department requires one for all products we use.

Asked by: J Thorpe
Thank you for your question. We do not provide SDS documents for our antibody reagents. SDSs are only provided for chemicals. Please reach out to your local distributor, they will be able to further assist you.
Answered by: Tech Service
Date published: 2020-01-28

What is the dilution used for immunohistochemistry (P)? Thank you

Asked by: marcelnani
Thank you for your question. We recommend a starting dilution of 1:50 for staining paraffin embedded sections with this antibody.
Answered by: Tech Service
Date published: 2021-06-04

For WB, does this antibody only recognizes native proteins? No SDS and reducing reagents in loading buffer?

Asked by: qinggoushi
Thank you for your question. This antibody, COL1A, sc-59772, will only recognize the native form, so yes, do not add SDS or reducing reagents to the loading buffer.
Answered by: Tech Service
Date published: 2019-11-13

If there any recommandation of secondary antibody for IF?

Asked by: lucky7
Thank you for your question. COL1A Antibody (COL-1) is a mouse monoclonal IgG1. We recommend using as a secondary antibody for IF, our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to a fluorescent probe such as fluorescein (FITC), m-IgGκ BP-FITC: sc-516140.
Answered by: Technical Support
Date published: 2019-04-13

Will this antibody stain for secreted fibrillar collagen-1? I'm unsure whether full-length native collagen type-1 means its specific for assembled collagen-1 fibrils. Will it also stain for pro-collagen chains? or only assembled fibrils? Thanks!

Asked by: Benn
Thank you for your question. This COL1A antibody recognises the native (helical) form of collagen type I.
Answered by: Tech Service
Date published: 2019-03-26

Hi, Can you supply conjugated version (either Alexa or PE) of this COL1A Antibody (COL-1): sc-59772? Thanks

Asked by: Harinath Sale
Thank you for your question. No, this antibody is provided in an ascites format and therefore is not available as a conjugated antibody. Please contact our Technical Service for recommended alternatives.
Answered by: Technical Support
Date published: 2018-12-13

Can I use this antibody for IHC-P on human skin tissue?

Asked by: moyaP
Thank you for your question. This antibody is recommended for IHC(P), or immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, and it is also suitable for detection of COL1A in human tissues. We have not yet tested the antibody in human skin tissue, however there are numerous publications citing use of this antibody for IF/IHC in human skin tissue.
Answered by: Technical Support
Date published: 2018-11-22
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Rated 5 out of 5 by from 出来结果不错我是上学时候用的抗体，很不错，现在工作了继续实验，又定了点抗体，价格可以，实验也满意，非常不错，赞。。
Date published: 2024-12-25
Rated 1 out of 5 by from no good for MEFI bought few weeks ago. I did western blot as soon as I got it. I used this antibody by dilution 1:500. There was no specific collagen band. I only tried MEF lysate. I'm not sure this antibody works for mouse collagen. If anybody knows antibodies for mouse collagen, please give me any information. Thanks,
Date published: 2018-05-11
Rated 5 out of 5 by from goodI tried this antibody in WB and got a very clear result
Date published: 2017-09-22
Rated 5 out of 5 by from for WBThis COL1A antibody by dilution 1:1000, WB results are good.
Date published: 2017-05-11
Rated 5 out of 5 by from specifically stains collagen IThis antibody specifically stains collagen I, which is an extra cellular matrix protein. As a result, the cell boundary of cardiomyocytes was clearly stained.
Date published: 2017-01-20
Rated 3 out of 5 by from Col 1a antibodyI bought few month ago and did western blot. I got some time good signal but these day I don't detect the signal. I have no idea why signal is suddenly disappear.
Date published: 2016-12-27
Rated 5 out of 5 by from Very good native detectionBest for native detection, and is specific for only COL1A
Date published: 2016-12-21
Rated 4 out of 5 by from No issues in our WesternNo issues in our Western. Good signal.
Date published: 2015-08-20
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COL1A 抗体 (COL-1) is rated 4.5 out of 5 by 13.
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