Date published: 2025-9-8
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coilin Antibody (F-7) - Western Blotting - Image 46141
coilin 抗体 (F-7): sc-55594. 未转染: sc-117752 (A) 和小鼠 coilin 转染: sc-125155 (B) 293T 全细胞裂解液和 MOLT-4 核提取物 (C) 中 coilin 表达的 Western 印迹分析.
coilin 抗体 (F-7): sc-55594. 未转染: sc-117752 (A) 和小鼠 coilin 转染: sc-125155 (B) 293T 全细胞裂解液和 MOLT-4 核提取物 (C) 中 coilin 表达的 Western 印迹分析.

coilin 抗体 (F-7): sc-55594

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说明书
  • coilin抗体(F-7)是小鼠单克隆IgG1 λ, 在20篇文献中引用，规格为200 µg/ml
  • 针对氨基酸277-576，在coilin的human起源的C-terminus处进行映射
  • 抗-coilin 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 coilin
  • 抗coilin抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取coilin (F-7): sc-55594的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 coilin 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与coilin 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Coilin抗体（F-7）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的coilin。抗Coilin抗体（F-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Coilin是一种重要的蛋白质，主要位于细胞核中，特别是位于卡哈尔体中，卡哈尔体是RNA转录和处理所必需的小核细胞器。Coilin在这些细胞器中的存在对于其形成和功能至关重要，因为Coilin有助于小核糖核蛋白（snRNPs）和生存运动神经元（SMN）复合蛋白的招募，而这些蛋白对于RNA剪接和基因调控至关重要。Coilin的自我结合能力和核仁定位信号凸显了其在维持卡雅体结构完整性方面的重要性。此外，Coilin的C端在决定细胞内卡雅体的数量方面发挥着调节作用，凸显了Coilin在细胞组织和功能中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

coilin 抗体 (F-7) 参考文献:

  1. 卡哈尔体：最初的 100 年。  |  Gall, JG. 2000. Annu Rev Cell Dev Biol. 16: 273-300. PMID: 11031238
  2. coilin的自我结合揭示了核体定位的一个共同主题。  |  Hebert, MD. and Matera, AG. 2000. Mol Biol Cell. 11: 4159-71. PMID: 11102515
  3. coilin基因敲除小鼠体内残留的Cajal体无法招募Sm snRNPs和脊髓性肌萎缩症基因产物SMN。  |  Tucker, KE., et al. 2001. J Cell Biol. 154: 293-307. PMID: 11470819
  4. Coilin 在 Cajal 体和脊髓性肌肉萎缩症蛋白 SMN 之间架起了桥梁。  |  Hebert, MD., et al. 2001. Genes Dev. 15: 2720-9. PMID: 11641277
  5. 植入前小鼠胚胎中功能性核仁的逐步组装涉及卡贾尔（盘绕）体。  |  Zatsepina, O., et al. 2003. Dev Biol. 253: 66-83. PMID: 12490198
  6. 一种新型核盘旋体蛋白的人类自身抗体：p80-coilin 的免疫学特征和 cDNA 克隆。  |  Andrade, LE., et al. 1991. J Exp Med. 173: 1407-19. PMID: 2033369
  7. 核仁定位的coilin受其低甲基化状态调节。  |  Tapia, O., et al. 2010. Chromosoma. 119: 527-40. PMID: 20449600
  8. Coilin与Ku蛋白相互作用，抑制体外非同源DNA末端连接。  |  Velma, V., et al. 2010. FEBS Lett. 584: 4735-9. PMID: 21070772
  9. Coilin 水平调节细胞周期进展和依托泊苷处理 U2OS 细胞中的 γH2AX 水平。  |  Velma, V., et al. 2012. FEBS Lett. 586: 3404-9. PMID: 22986342
  10. Coilin 迅速被招募到 UVA 诱导的 DNA 损伤中，γ-辐射影响 Cajal 体的局部运动。  |  Bártová, E., et al. 2014. Nucleus. 5: 460-8. PMID: 24859326
  11. 人类 p80-coilin 基因的结构, 表达和染色体定位。  |  Chan, EK., et al. 1994. Nucleic Acids Res. 22: 4462-9. PMID: 7971277
  12. Cajal体的数量由coilin C-端介导控制。  |  Shpargel, KB., et al. 2003. J Cell Sci. 116: 303-12. PMID: 12482916

订购信息

产品名称产品编号规格价格数量收藏夹

coilin 抗体 (F-7)

sc-55594
200 µg/ml
RMB2377.00

coilin (F-7): m-IgG1 BP-HRP 套装

sc-531646
200 µg Ab; 20 µg BP
RMB2662.00

coilin 抗体 (F-7) AC

sc-55594 AC
500 µg/ml, 25% agarose
RMB3129.00

coilin 抗体 (F-7) HRP

sc-55594 HRP
200 µg/ml
RMB2377.00

coilin 抗体 (F-7) FITC

sc-55594 FITC
200 µg/ml
RMB2482.00

coilin 抗体 (F-7) PE

sc-55594 PE
200 µg/ml
RMB2580.00

coilin 抗体 (F-7) Alexa Fluor® 488

sc-55594 AF488
200 µg/ml
RMB2685.00

coilin 抗体 (F-7) Alexa Fluor® 546

sc-55594 AF546
200 µg/ml
RMB2685.00

coilin 抗体 (F-7) Alexa Fluor® 594

sc-55594 AF594
200 µg/ml
RMB2685.00

coilin 抗体 (F-7) Alexa Fluor® 647

sc-55594 AF647
200 µg/ml
RMB2685.00

coilin 抗体 (F-7) Alexa Fluor® 680

sc-55594 AF680
200 µg/ml
RMB2685.00

coilin 抗体 (F-7) Alexa Fluor® 790

sc-55594 AF790
200 µg/ml
RMB2685.00

What dilution of this antibody do you recommend for IF? U2OS cells! Thanks!

Asked by: Singh
Thank you for your question. We recommend a starting dilution of 1:50-1:500. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-05-24

Any chance of ordering carrier-free versions of this antibody?

Asked by: apmagalhaes
Thank you for your question. Please get in touch with our technical service team so we can look into your requirement.
Answered by: Tech Support Europe
Date published: 2023-11-27

I am using coilin (F-7): sc-55594 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

Hello, I wanted to ask if there would be any recommendations on using triton to fix the cells?

Asked by: ps95
Thank you for your inquiry. If you are using formalin to fix your cells, then we do recommend using Triton to permeabilize your cells. After fixation, wash in 3 changes of PBS for 5 minutes each. Then, add a solution of .01% Triton X-100 in PBS and incubate for 3 minutes. Wash in 3 changes of PBS for 5 minutes each. Then proceed with blocking. A link to our recommended protocol for immunofluorescence cell staining is provided for your refernece. https://www.scbt.com/scbt/resources/protocols/immunoflourescence-cell-staining
Answered by: Technical Support
Date published: 2016-11-08
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Rated 4 out of 5 by from Immunofluorescence stainingThis antibody worked well in immunofluorescent staining of fixed HeLa cells
Date published: 2024-11-08
Rated 5 out of 5 by from HCT116細胞での免疫染色とウェスタンブロットできました。HCT116細胞の免疫染色とウェスタンブロットできました。免疫染色に関しては、HeLa細胞とMCF7細胞でOKでした。なお、ウェスタンブロットは、Pdeltaよりも非特異的なバンドが少ない印象でした。
Date published: 2019-04-17
Rated 5 out of 5 by from strong band seen in WBThis antibody detect the target coilin in HeLa cell lysate, with 80KD specific band. It work well in western blot at 1:1000.
Date published: 2017-11-13
Rated 4 out of 5 by from Marche bien en IFAnticorps marche super bien, testé au 1:100e en IF sur cellules fixées au PFA3% 15min
Date published: 2017-11-07
Rated 5 out of 5 by from specific bandsThis antibody detect the target coilin in human liver tumor tissue extracts .It shows specific bands at 80kda.I suggest controled the dilution between 1:400 to 1:1000.Futher diluted rate such as 1:1500 shows very weak signal.
Date published: 2017-09-09
Rated 5 out of 5 by from used for WBThis antibody titer is very high, it suitable for WB detection of coilin protein, .1:800 dilued the antibody can got strong signal,very cost-effective antibody.
Date published: 2017-07-22
Rated 5 out of 5 by from excellent antibody for IFThis antibody works very well for IF (a very strong signal even when used at 1:750 dilution). It also works for WB at 1:500 dilution (I suspect the signal would be stronger at lower dilutions).
Date published: 2017-07-05
Rated 5 out of 5 by from strong band seenGreat band obtained in WB on human intervertebral disc specimers,very strong band seen.
Date published: 2017-05-24
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coilin 抗体 (F-7) is rated 4.9 out of 5 by 14.
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