Date published: 2025-9-7
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Clusterin-α Antibody (B-5) - Western Blotting - Image 378859
Clusterin-α 抗体 (B-5): sc-5289. SK-BR-3 (A), Caco-2 (B), PANC-1 (C) 和 A549 (D) 全细胞裂解液中 Clusterin-α 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Clusterin-α 抗体 (B-5): sc-5289. SK-BR-3 (A), Caco-2 (B), PANC-1 (C) 和 A549 (D) 全细胞裂解液中 Clusterin-α 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Clusterin-α 抗体 (B-5): sc-5289

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说明书
  • Clusterin-alpha 抗体 B-5 是小鼠单克隆 IgG1 κ，Clusterin-α抗体, 在52篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Clusterin-α的C-terminus的氨基酸432-449之间
  • Clusterin-alpha 抗体 (B-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Clusterin-α
  • 抗Clusterin-alpha抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Clusterin-α (B-5): sc-5289的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Clusterin-α 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Clusterin-α 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Clusterin 抗体

Clusterin-α 抗体（B-5）是一种小鼠单克隆 IgG1 kappa 轻链抗体，专门针对人类 Clusterin-α，可识别 C 末端 432-449 氨基酸之间的表位。Clusterin-α 单克隆抗体（B-5）可用于免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋组织免疫组织化学（IHCP）和酶联免疫吸附（ELISA）。Clusterin-α，也称为载脂蛋白J（ApoJ），是一种多功能分泌性糖蛋白，参与多种重要的生理过程，包括免疫调节、细胞粘附、脂质运输、组织重塑、膜回收和细胞间相互作用。Clusterin-α作为补体裂解抑制剂，对于保护细胞免受补体介导的损伤至关重要，从而维持免疫稳态。此外，Clusterin-α与多种蛋白质相互作用，影响细胞凋亡、神经保护和淀粉样β肽聚集等过程，而淀粉样β肽与阿尔茨海默病的发病机制有关。Clusterin-α的异二聚体结构由α和β亚基通过二硫键连接而成，对于稳定性和多功能性至关重要。翻译后修饰，包括糖基化和蛋白水解，对于Clusterin-α的正常折叠、分泌和活性至关重要，会影响其在不同细胞环境中的相互作用和功能结果。在包括癌症在内的多种病理条件下，Clusterin-α的表达水平会升高，其中Clusterin-α与肿瘤进展和转移有关；在神经退行性疾病中，Clusterin-α可能发挥保护或病理作用，具体取决于环境。通过特异性靶向Clusterin-α的C端区域，Clusterin-α (B-5)单克隆抗体有助于对不同生物系统中的表达、定位和相互作用进行详细研究，从而为健康和疾病中的角色提供宝贵的见解。Clusterin-α小鼠单克隆抗体（B-5）推荐用于研究免疫调节、癌症生物学、神经退行性疾病和治疗策略的开发。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Clusterin-α 抗体 (B-5) 参考文献:

  1. 载脂蛋白 J/clusterin 可预防衰老引起的渐进性肾小球病变。  |  Rosenberg, ME., et al. 2002. Mol Cell Biol. 22: 1893-902. PMID: 11865066
  2. 细胞死亡蛋白--核集束蛋白的合成与功能分析。  |  Leskov, KS., et al. 2003. J Biol Chem. 278: 11590-600. PMID: 12551933
  3. 作为小鼠和人类肠道肿瘤生物标志物的群集素  |  Chen, X., et al. 2003. Proc Natl Acad Sci U S A. 100: 9530-5. PMID: 12886021
  4. 人类结肠肿瘤发生过程中不同群集素异构体的调节。  |  Pucci, S., et al. 2004. Oncogene. 23: 2298-304. PMID: 14755245
  5. 压力诱导缺血后大鼠视网膜 Müller 细胞中簇蛋白的表达。  |  Gwon, JS., et al. 2004. Glia. 47: 35-45. PMID: 15139011
  6. 人类卵巢癌中细胞质群集素的上调表达。  |  Xie, D., et al. 2005. Cancer. 103: 277-83. PMID: 15578711
  7. 胰岛素样生长因子-1受体/Src/MAPK/Egr-1信号传导的延迟激活可调控簇蛋白的表达，而簇蛋白是一种促生存因子。  |  Criswell, T., et al. 2005. J Biol Chem. 280: 14212-21. PMID: 15689620
  8. Clusterin 通过与活化的 Bax 相互作用来抑制细胞凋亡。  |  Zhang, H., et al. 2005. Nat Cell Biol. 7: 909-15. PMID: 16113678
  9. 载脂蛋白 J：结构和组织分布。  |  de Silva, HV., et al. 1990. Biochemistry. 29: 5380-9. PMID: 1974459

订购信息

产品名称产品编号规格价格数量收藏夹

Clusterin-α 抗体 (B-5)

sc-5289
200 µg/ml
RMB2377.00

Clusterin-α (B-5): m-IgG Fc BP-HRP 套装

sc-528146
200 µg Ab; 10 µg BP
RMB2662.00

Clusterin-α (B-5): m-IgGκ BP-HRP 套装

sc-520453
200 µg Ab, 40 µg BP
RMB2662.00

Clusterin-α (B-5): m-IgG1 BP-HRP 套装

sc-542787
200 µg Ab; 20 µg BP
RMB2662.00

Clusterin-α 抗体 (B-5) AC

sc-5289 AC
500 µg/ml, 25% agarose
RMB3129.00

Clusterin-α 抗体 (B-5) HRP

sc-5289 HRP
200 µg/ml
RMB2377.00

Clusterin-α 抗体 (B-5) FITC

sc-5289 FITC
200 µg/ml
RMB2482.00

Clusterin-α 抗体 (B-5) PE

sc-5289 PE
200 µg/ml
RMB2580.00

Clusterin-α 抗体 (B-5) Alexa Fluor® 488

sc-5289 AF488
200 µg/ml
RMB2685.00

Clusterin-α 抗体 (B-5) Alexa Fluor® 546

sc-5289 AF546
200 µg/ml
RMB2685.00

Clusterin-α 抗体 (B-5) Alexa Fluor® 594

sc-5289 AF594
200 µg/ml
RMB2685.00

Clusterin-α 抗体 (B-5) Alexa Fluor® 647

sc-5289 AF647
200 µg/ml
RMB2685.00

Clusterin-α 抗体 (B-5) Alexa Fluor® 680

sc-5289 AF680
200 µg/ml
RMB2685.00

Clusterin-α 抗体 (B-5) Alexa Fluor® 790

sc-5289 AF790
200 µg/ml
RMB2685.00

Clusterin-α (B-5) 中和勝肽

sc-5289 P
100 µg/0.5 ml
RMB511.00

whether this antibody could be used for mouse tissues.

Asked by: Anonymous
Thank you for your question. Clusterin-alpha Antibody (B-5) is only recommended for detection of Clusterin-α of human origin by WB, IP, IF, IHC(P) and ELISA. It is not suitable for cell or tissue samples from mouse.
Answered by: Tech Support Europe
Date published: 2021-01-27

The protein band detected as clusterin-alpha percursor corresponds to the percursor containing both the N-signal and alpha and beta sequences right?

Asked by: jvsilva
Thank you for your question. Clusterin-α Antibody (B-5): sc-5289 recognizes amino acids 432-449, which correspond to Clusterin alpha chain. As such, the antibody detects any form of Clusterin containing this sequence.
Answered by: Technical Support Europe
Date published: 2020-10-16

I want to ask why many clusterin antibody are clusterin-alpha chain antibody? not alpha and beta chain together, or only beta chain? Does it really can identify clusterin protein? Don't know how to choose the suitable clusterin antibody.

Asked by: Rachelwong
Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, α and β, are associated through disulfide bonds. The β subunit (also called ApoJα) corresponds to residues 23-227. The α subunit (also called ApoJβ) corresponds to residues 228-449. Based on epitope mapping of the antibody, it is possible to make antibodies that are specific to either the alpha or beta chain. If the epitope has not been mapped, then the antibodies may be recommended for detection of Clusterin protein.
Answered by: Technical Support
Date published: 2018-12-11

hello, i want to detect to nuclear clusterin as IHC-P and WB. So can i use Clusterin-α Antibody (B-5): sc-5289 ? Because i see H-330 doesent product anymore.

Asked by: histolog
Thank you for your question. Yes, our mouse monoclonal antibody, Clusterin-α (B-5): sc-5289, is recommended for detection of Clusterin-α of human origin by WB, IP, IF, IHC(P) and ELISA. The rabbit polyclonal (H-330) antibody has been discontinued, and this is the suggested replacement. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department to request a sample. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-30

How should the blocking peptide sc-5289 P be used?

Asked by: jenni
Thank you for your question. The blocking peptide is intended to be used as a negative control by preadsorbing the Clusterin-α (B-5): sc-5289 with it prior to application. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 13
Date published: 2017-01-20

How can Clusterin-α (B-5): sc-5289 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Clusterin-α (B-5): sc-5289 monoclonal antibody is currently available conjugated to phycoerythrin (sc-5289 PE), fluorescein (sc-5289 FITC), Alexa Fluor® 488 (sc-5289 AF488) or Alexa Fluor® 647 (sc-5289 AF647)
Answered by: Technical Support 9
Date published: 2017-01-16
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Rated 5 out of 5 by from Workします。ラットの心臓のバンドがきれいに出ました。きれいなバンドが出たので、実験が進みます。大変助かりました。
Date published: 2024-01-06
Rated 4 out of 5 by from WB for human cell lineWhole cell lysate from HeLa cells and ARPE-19 cells. 1/1000 dilution in 3% SM, O/N at 4 degree. Clear bands were detected.
Date published: 2022-12-21
Rated 4 out of 5 by from Good specificityWorked great for a sample that had a very small protein concentration
Date published: 2019-08-21
Rated 5 out of 5 by from good stainingGood nuclear signal in immunostaining of embryonic cardiomyocytes (mouse).
Date published: 2017-02-25
Rated 5 out of 5 by from Great Clusterin-alpha specificityGreat antibody with known specificity to Clusterin-alpha through siRNA knockdown data. Strong staining in IHC and robust bands in WB.
Date published: 2017-01-30
Rated 5 out of 5 by from Great WB and IHCOutstanding product; take a look at IHC staining data; as good as it gets !!
Date published: 2017-01-26
Rated 4 out of 5 by from Positive detection of both the precursorPositive detection of both the precursor and mature forms of the Clusterin- protein by WB using SK-BR-3 whole cell lysate. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-29
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Clusterin-α 抗体 (B-5) is rated 4.8 out of 5 by 12.
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