Date published: 2025-9-8

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CLIP-170 抗体 (F-3): sc-28325

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说明书
  • CLIP-170 抗体 F-3 是小鼠单克隆 IgG2b κ,CLIP-170抗体, 在13篇文献中引用,规格为200 µg/ml
  • 免疫human物种的CLIP-170的氨基酸1128-1427
  • CLIP-170 抗体 (F-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 CLIP-170
  • 抗CLIP-170抗体(F-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CLIP-170 (F-3): sc-28325免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 CLIP-170 抗体 (F-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CLIP-170 抗体 (F-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CLIP-170抗体(F-3)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类CLIP-170蛋白。CLIP-170 (F-3) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。细胞质连接蛋白(CLIP-170)作为加端结合蛋白(+TIP)的一员在细胞动力学中发挥着至关重要的作用,而加端结合蛋白对于微管的稳定和组织至关重要。CLIP-170通过与不断生长的微管正端结合,成为这些动态结构和细胞器膜之间的连接体,从而促进微管的修复并防止灾难的发生,特别是在细胞周边区域。这一功能对于维持细胞完整性和促进细胞内运输至关重要,因为CLIP-170可确保微管在细胞分裂和细胞器移动等细胞过程中保持稳定和功能。此外,荧光标记的CLIP-170可以在微管生长末端以彗星状条纹的形式显现,为活细胞成像研究中的动态行为提供了洞察力。CLIP-170还与微管末端和动点上的动力蛋白和动力蛋白复合物共定位,凸显了CLIP-170在细胞运输和分裂协调中的重要性。此外,CLIP-170在霍奇金和里德-斯特恩伯格(HRS)细胞、单核细胞衍生树突状细胞、白介素4加CD40配体激活的B细胞和Ki-1淋巴瘤中高度表达,这使得CLIP-170成为各种病理状况的重要标志物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CLIP-170 抗体 (F-3) 参考文献:

  1. CLIP-170 可突出显示体内不断生长的微管末端。  |  Perez, F., et al. 1999. Cell. 96: 517-27. PMID: 10052454
  2. 细胞质连接蛋白 CLIP-170 是一种人类自身抗原。  |  Griffith, KJ., et al. 2002. Clin Exp Immunol. 127: 533-8. PMID: 11966772
  3. 霍奇金和里德-斯滕伯格细胞相关自身抗原CLIP-170/restin是树突状细胞的标记,并参与大体小体向细胞骨架的贩运,支持霍奇金和里德-斯滕伯格细胞基于功能的概念。  |  Sahin, U., et al. 2002. Blood. 100: 4139-45. PMID: 12433698
  4. 细胞质连接蛋白可促进体内微管的修复。  |  Komarova, YA., et al. 2002. J Cell Biol. 159: 589-99. PMID: 12446741
  5. CLIP-170 通过不同的机制与动态蛋白复合物和 APC 结合蛋白 EB1 相互作用。  |  Goodson, HV., et al. 2003. Cell Motil Cytoskeleton. 55: 156-73. PMID: 12789661
  6. CLIP-170 将内细胞囊泡与微管连接起来。  |  Pierre, P., et al. 1992. Cell. 70: 887-900. PMID: 1356075
  7. 细胞质连接蛋白 CLIP-170 的最小加端跟踪单元。  |  Gupta, KK., et al. 2009. J Biol Chem. 284: 6735-42. PMID: 19074770
  8. 细胞质连接蛋白 CLIP170 通过靶向 TLR 适配蛋白 TIRAP 对 TLR4 信号进行负调控。  |  Jakka, P., et al. 2018. J Immunol. 200: 704-714. PMID: 29222167
  9. Dynactin 1通过封存宿主辅助因子CLIP170来负向调节HIV-1感染。  |  Shanmugapriya, S., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34686593
  10. 孕烯醇酮-甲醚能增强CLIP170和微管功能,改善脊柱成熟和与CDKL5缺乏有关的海马缺陷。  |  Barbiero, I., et al. 2022. Hum Mol Genet. 31: 2738-2750. PMID: 35348691
  11. CLIP170 通过 TGF-β 通路抑制甲状腺乳头状癌的转移和 EMT。  |  Ma, B., et al. 2024. Med Oncol. 41: 137. PMID: 38705933
  12. 霍奇金病和无性大细胞淋巴瘤中的 Restin。  |  Delabie, J., et al. 1993. Leuk Lymphoma. 12: 21-6. PMID: 8161933

订购信息

产品名称产品编号规格价格数量收藏夹

CLIP-170 抗体 (F-3)

sc-28325
200 µg/ml
RMB2377.00

CLIP-170 (F-3): m-IgG Fc BP-HRP 套装

sc-525520
200 µg Ab; 10 µg BP
RMB2662.00

CLIP-170 (F-3): m-IgG2b BP-HRP 套装

sc-549569
200 µg Ab; 10 µg BP
RMB2662.00

CLIP-170 抗体 (F-3) AC

sc-28325 AC
500 µg/ml, 25% agarose
RMB3129.00

CLIP-170 抗体 (F-3) HRP

sc-28325 HRP
200 µg/ml
RMB2377.00

CLIP-170 抗体 (F-3) FITC

sc-28325 FITC
200 µg/ml
RMB2482.00

CLIP-170 抗体 (F-3) PE

sc-28325 PE
200 µg/ml
RMB2580.00

CLIP-170 抗体 (F-3) Alexa Fluor® 488

sc-28325 AF488
200 µg/ml
RMB2685.00

CLIP-170 抗体 (F-3) Alexa Fluor® 546

sc-28325 AF546
200 µg/ml
RMB2685.00

CLIP-170 抗体 (F-3) Alexa Fluor® 594

sc-28325 AF594
200 µg/ml
RMB2685.00

CLIP-170 抗体 (F-3) Alexa Fluor® 647

sc-28325 AF647
200 µg/ml
RMB2685.00

CLIP-170 抗体 (F-3) Alexa Fluor® 680

sc-28325 AF680
200 µg/ml
RMB2685.00

CLIP-170 抗体 (F-3) Alexa Fluor® 790

sc-28325 AF790
200 µg/ml
RMB2685.00

Can CLIP-170 (F-3): sc-28325 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CLIP-170 (F-3): sc-28325 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent antibody!We have tested this antibody by western blot and it works great!
Date published: 2018-02-22
Rated 4 out of 5 by from worked well in western blotThis antibody worked very well in western blot on mouse osteoclasts extract.
Date published: 2017-10-25
Rated 3 out of 5 by from good antibodyClear band in Hela cells.Used at the dilution of 1:1000.
Date published: 2017-10-24
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of CLIP-170 expression in JAR, HUV-EC-C, HeLa, Hep G2, MCF7 and RAW 264.7 whole cell lysates.
Date published: 2017-01-30
Rated 5 out of 5 by from Worked great in IF in mouse cellsWorked great in IF in mouse cells.
Date published: 2015-09-29
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from CleanClean, clear bands present in western blot analysis of CLIP-170 expression in T2.DR3 and KNRK whole cell lysates and mouse lung tissue extract. -SCBT QC
Date published: 2015-01-12
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CLIP-170 抗体 (F-3) is rated 4.6 out of 5 by 7.
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