Date published: 2025-9-7
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Clathrin Heavy Chain/CLTC Antibody (TD.1) - Western Blotting - Image 353302
Clathrin HC 抗体 (TD. 1): sc-12734. 对 Raji (A), SH-SY5Y (B), Hep G2 (C), EOC 20 (D), PC-12 (E) 和 C6 (F) 全细胞裂解液中 Clathrin HC 表达的 Western 印迹分析.
Clathrin HC 抗体 (TD. 1): sc-12734. 对 Raji (A), SH-SY5Y (B), Hep G2 (C), EOC 20 (D), PC-12 (E) 和 C6 (F) 全细胞裂解液中 Clathrin HC 表达的 Western 印迹分析.

Clathrin Heavy Chain/CLTC 抗体 (TD.1): sc-12734

4.5(26)
寫評論提問

说明书
  • Clathrin Heavy Chain/CLTC 抗体 TD.1 是小鼠单克隆 IgG1 κ，Clathrin Heavy Chain/CLTC抗体, 在89篇文献中引用，规格为200 µg/ml
  • 针对Clathrin HC的human起源的N-terminus的
  • Clathrin Heavy Chain/CLTC 抗体 (TD.1) 推荐用于WB, IP, IF 和 IHC(P)，检测broad species 来源的 Clathrin HC
  • 抗Clathrin Heavy Chain/CLTC抗体（TD.1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Clathrin Heavy Chain/CLTC (TD.1): sc-12734的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Clathrin Heavy Chain/CLTC 抗体 (TD.1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Clathrin Heavy Chain/CLTC 抗体 (TD.1) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

额外Clathrin抗体，包括Clathrin LC

Clathrin HC抗体（TD.1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测多种物种来源的Clathrin HC蛋白。抗Clathrin HC抗体（TD.1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Clathrin在细胞内运输中起着至关重要的作用，特别是在受体介导的内吞作用中，Clathrin有助于将受体蛋白吞入Clathrin包被的囊泡中。Clathrin主要位于细胞质中，在那里Clathrin组装成三叶形结构，由三个重链和三个轻链组成。这种独特的结构对于细胞内运输蛋白质和脂质的囊泡的形成至关重要，凸显了Clathrin在维持细胞内稳态和通讯中的重要性。Clathrin重链的近端包含一个轻链结合位点，这对于三叶形组装和随后形成包被Clathrin的囊泡至关重要。Clathrin HC (TD.1) 抗体使研究人员能够有效地研究由Clathrin介导的内吞作用的动力学及其在各种细胞过程中的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Clathrin Heavy Chain/CLTC 抗体 (TD.1) 参考文献:

  1. Clathrin Heavy Chain Subunits Coordinate Endo- and Exocytic Traffic and Affect Stomatal Movement.  |  Larson, ER., et al. 2017. Plant Physiol. 175: 708-720. PMID: 28830938
  2. 包膜囊泡的结构和组装。  |  Pearse, BM. and Crowther, RA. 1987. Annu Rev Biophys Biophys Chem. 16: 49-68. PMID: 2885011
  3. Clathrin 和包被囊泡。  |  Pearse, BM. 1987. EMBO J. 6: 2507-12. PMID: 2890519
  4. Clathrin 重链敲除影响 CXCR4 信号传导和翻译后修饰  |  DeNies, MS., et al. 2019. Front Cell Dev Biol. 7: 77. PMID: 31139626
  5. 在T606位点被磷酸化后，Clathrin heavy chain在正常的细胞分裂中发挥作用。  |  Yabuno, Y., et al. 2019. Cell Cycle. 18: 1976-1994. PMID: 31272276
  6. Clathrin heavy chain的替代性剪接有助于从包被小坑向斑块的转变。  |  Moulay, G., et al. 2020. J Cell Biol. 219: PMID: 32642759
  7. 人类凝集素轻链的结构。三种哺乳动物轻链多态性的一致性。  |  Jackson, AP. and Parham, P. 1988. J Biol Chem. 263: 16688-95. PMID: 3267234
  8. Clathrin 重链：分子克隆和完整的一级结构。  |  Kirchhausen, T., et al. 1987. Proc Natl Acad Sci U S A. 84: 8805-9. PMID: 3480512
  9. 低复杂度RGG-motif包含蛋白Scd6和Psp2作为抑制剂，抑制了clathrin heavy chain的缺陷。  |  Garg, M., et al. 2022. Biochim Biophys Acta Mol Cell Res. 1869: 119327. PMID: 35901970
  10. 利用重组的三疱疹病毒枢纽确定凝集素组装和三聚化的调控。  |  Liu, SH., et al. 1995. Cell. 83: 257-67. PMID: 7585943
  11. 溶酶体膜蛋白的胞内运输。  |  Hunziker, W. and Geuze, HJ. 1996. Bioessays. 18: 379-89. PMID: 8639161
  12. 内吞和分子分拣。  |  Mellman, I. 1996. Annu Rev Cell Dev Biol. 12: 575-625. PMID: 8970738

订购信息

产品名称产品编号规格价格数量收藏夹

Clathrin Heavy Chain/CLTC 抗体 (TD.1)

sc-12734
200 µg/ml
RMB2377.00

Clathrin Heavy Chain/CLTC (TD.1): m-IgG Fc BP-HRP 套装

sc-528253
200 µg Ab; 10 µg BP
RMB2662.00

Clathrin Heavy Chain/CLTC (TD.1): m-IgGκ BP-HRP 套装

sc-520583
200 µg Ab, 40 µg BP
RMB2662.00

Clathrin Heavy Chain/CLTC (TD.1): m-IgG1 BP-HRP 套装

sc-542842
200 µg Ab; 20 µg BP
RMB2662.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) AC

sc-12734 AC
500 µg/ml, 25% agarose
RMB3129.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) HRP

sc-12734 HRP
200 µg/ml
RMB2377.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) FITC

sc-12734 FITC
200 µg/ml
RMB2482.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) PE

sc-12734 PE
200 µg/ml
RMB2580.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) Alexa Fluor® 488

sc-12734 AF488
200 µg/ml
RMB2685.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) Alexa Fluor® 546

sc-12734 AF546
200 µg/ml
RMB2685.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) Alexa Fluor® 594

sc-12734 AF594
200 µg/ml
RMB2685.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) Alexa Fluor® 647

sc-12734 AF647
200 µg/ml
RMB2685.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) Alexa Fluor® 680

sc-12734 AF680
200 µg/ml
RMB2685.00

Clathrin Heavy Chain/CLTC 抗体 (TD.1) Alexa Fluor® 790

sc-12734 AF790
200 µg/ml
RMB2685.00

Hello, I was hoping to get more information on which isoform and amino acid region of CLTC this antibody was made against?

Asked by: CLTC epitope
Thank you for your question. Clathrin Heavy Chain/CLTC Antibody (TD.1): sc-12734 raised against the N-terminus of Clathrin HC of human origin. No epitope mapping has been performed for this antibody as of yet.
Answered by: Tech Support Europe
Date published: 2025-09-01

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Clathrin HC Antibody (TD.1): sc-12734?

Asked by: sweetums
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-12

Can Clathrin HC Antibody (TD.1): sc-12734 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: sweetums
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Clathrin HC Antibody (TD.1): sc-12734 monoclonal antibody is currently available conjugated to HRP (sc-12734 HRP), phycoerythrin (sc-12734 PE), fluorescein (sc-12734 FITC), Alexa Fluor® 488 (sc-12734 AF488), and Alexa Fluor® 647 (sc-12734 AF647).
Answered by: Technical Support 13
Date published: 2017-01-12
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Rated 5 out of 5 by from GOOD FOR WBGood Western Blotting signal in Hela whole cell lysate
Date published: 2025-06-11
Rated 5 out of 5 by from きれいにバンドがでました約1ヶ月前にサンプル品をもらい、製品も購入しました。写真はサンプル品でのものです。sup画分できれいにバンドが出ました。ノンスペは殆どありませんでした。
Date published: 2023-06-29
Rated 5 out of 5 by from Immunoblotting of Clathrin heavy chainWe have tested the anti-Clathrin antibody (sc-12734) from Santa Cruz Biotechnology by immunoblotting. We have obtained excellent results in various human and mouse cell lines.
Date published: 2022-09-08
Rated 5 out of 5 by from Excellent for immunofluorescenceI tested this antibody for IF in methanol-fixed VeroE6 and Huh-7 cells. Dil: 1/200, secondary: antimouse Alexa488 (Invitrogen A21202). Excellent clathrin staining. Attached image was collected on VeroE6 cells by confocal Zeiss 800 (scale bar: 10 um).
Date published: 2020-11-26
Rated 4 out of 5 by from Work Well for IFI tried the anti Clathrin antibody for IF in FRTL-5 cells (rat cell line) and MDCK cells (dog cell line) and it worked perfectly fine!
Date published: 2018-02-23
Rated 5 out of 5 by from Works very well for western blotConfirmed strong knockdown of Clathrin by siRNA by western
Date published: 2018-02-10
Rated 4 out of 5 by from Worked for IHCI used it for IHC on mouse retina frozen section (10 um) at 1/100 dilution. I did not test it by western blot .
Date published: 2017-11-06
Rated 3 out of 5 by from Not suitable for colocalization with light chainWe asked this CHC antibody from Santa, we tested it by IF in MDCK cells, it decorated the cells with clearly fluorescent spots, unfortunately, we failed to get a highly colocalized picture with CLCa (a light chain of clathrin complex)
Date published: 2017-10-07
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Clathrin Heavy Chain/CLTC 抗体 (TD.1) is rated 4.5 out of 5 by 27.
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