Date published: 2025-9-7
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CKR-5 Antibody (D-6) - Western Blotting - Image 66884
CKR-5 抗体 (D-6): sc-17833. 293T 全细胞裂解液中 CKR-5 表达的 Western 印迹分析.
CKR-5 抗体 (D-6): sc-17833. 293T 全细胞裂解液中 CKR-5 表达的 Western 印迹分析.

CKR-5 抗体 (D-6): sc-17833

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说明书
  • CKR-5 抗体 D-6 是小鼠单克隆 IgG1 κ，CKR-5抗体, 在17篇文献中引用，规格为200 µg/ml
  • 免疫human物种的C-C chemokine receptor gene 5 (CKR-5)的氨基酸66-250
  • CKR-5 抗体 (D-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CKR-5
  • 抗CKR-5抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CKR-5 (D-6): sc-17833的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CKR-5 Antibody (D-6) for WB applications. 的首选辅助检测试剂，这些试剂现与CKR-5 Antibody (D-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外C-C Chemokine Receptor抗体，包括CKR-10, CKR-2, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-7, CKR-9和Chemokine Receptor D6

CKR-5抗体（D-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的CKR-5蛋白。CKR-5 (D-6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。CKR-5蛋白，也称为CCR5，是C-C趋化因子受体家族的一员，通过介导T细胞和单核细胞向炎症部位的趋化作用，在免疫反应中发挥关键作用。 CKR-5作为HIV的共同受体，促进病毒进入宿主细胞，特别是巨噬细胞趋化的病毒株。这种相互作用凸显了CKR-5在正常免疫功能和HIV感染病理学中的重要性，使CKR-5成为阻断病毒进入的治疗干预的关键靶点。CKR-5的结构由七个跨膜结构域组成，能够有效传递信号，响应趋化因子的结合，这对于协调免疫反应至关重要。在研究中使用抗CKR-5抗体（D-6）可以深入了解免疫细胞迁移机制和CKR-5在病毒发病机理中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CKR-5 抗体 (D-6) 参考文献:

  1. 克隆人类和小鼠 EBI1，这是一种淋巴特异性 G 蛋白偶联受体，在人类染色体 17q12-q21.2 上编码。  |  Schweickart, VL., et al. 1994. Genomics. 23: 643-50. PMID: 7851893
  2. HIV-1 进入辅助因子：七跨膜 G 蛋白偶联受体的功能性 cDNA 克隆。  |  Feng, Y., et al. 1996. Science. 272: 872-7. PMID: 8629022
  3. 鉴定 HIV-1 原始分离株的主要共受体。  |  Deng, H., et al. 1996. Nature. 381: 661-6. PMID: 8649511
  4. HIV-1 进入 CD4+ 细胞是由趋化因子受体 CC-CKR-5 介导的。  |  Dragic, T., et al. 1996. Nature. 381: 667-73. PMID: 8649512
  5. CC CKR5：RANTES, MIP-1alpha, MIP-1beta 受体，是巨噬细胞趋向性 HIV-1 的融合辅助因子。  |  Alkhatib, G., et al. 1996. Science. 272: 1955-8. PMID: 8658171
  6. 乙型促凝血因子受体 CCR3 和 CCR5 有助于初级 HIV-1 分离物的感染。  |  Choe, H., et al. 1996. Cell. 85: 1135-48. PMID: 8674119
  7. 一种使用 fusin 和 β-趋化因子受体 CKR-5, CKR-3 和 CKR-2b 作为融合辅助因子的双向初代 HIV-1 分离物。  |  Doranz, BJ., et al. 1996. Cell. 85: 1149-58. PMID: 8674120
  8. CKR-5缺失杂合子的艾滋病进展较慢。  |  Rowe, PM. 1996. Lancet. 348: 947. PMID: 8843818
  9. 1 型人类免疫缺陷病毒的巨噬细胞趋向性和 CC-CKR5 核心受体的利用。  |  Cheng-Mayer, C., et al. 1997. J Virol. 71: 1657-61. PMID: 8995695
  10. CKR-5基因缺失的杂合子导致一组艾滋病毒血清反应阳性者无艾滋病生存期延长，CD4 T细胞下降速度减慢。  |  Eugen-Olsen, J., et al. 1997. AIDS. 11: 305-10. PMID: 9147421
  11. CD34+ 造血祖细胞中人体免疫缺陷病毒 1 型核心受体 CXCR-4 （fusin、LESTR）和 CKR-5 的表达。  |  Deichmann, M., et al. 1997. Blood. 89: 3522-8. PMID: 9160656
  12. 鉴定新型淋巴细胞定向 CC 趋化因子 LARC 的特异性受体 CCR6。  |  Baba, M., et al. 1997. J Biol Chem. 272: 14893-8. PMID: 9169459

订购信息

产品名称产品编号规格价格数量收藏夹

CKR-5 抗体 (D-6)

sc-17833
200 µg/ml
RMB2377.00

CKR-5 (D-6): m-IgG Fc BP-HRP 套装

sc-526577
200 µg Ab; 10 µg BP
RMB2662.00

CKR-5 (D-6): m-IgG1 BP-HRP 套装

sc-531950
200 µg Ab; 20 µg BP
RMB2662.00

CKR-5 抗体 (D-6) AC

sc-17833 AC
500 µg/ml, 25% agarose
RMB3129.00

CKR-5 抗体 (D-6) HRP

sc-17833 HRP
200 µg/ml
RMB2377.00

CKR-5 抗体 (D-6) FITC

sc-17833 FITC
200 µg/ml
RMB2482.00

CKR-5 抗体 (D-6) PE

sc-17833 PE
200 µg/ml
RMB2580.00

CKR-5 抗体 (D-6) Alexa Fluor® 488

sc-17833 AF488
200 µg/ml
RMB2685.00

CKR-5 抗体 (D-6) Alexa Fluor® 546

sc-17833 AF546
200 µg/ml
RMB2685.00

CKR-5 抗体 (D-6) Alexa Fluor® 594

sc-17833 AF594
200 µg/ml
RMB2685.00

CKR-5 抗体 (D-6) Alexa Fluor® 647

sc-17833 AF647
200 µg/ml
RMB2685.00

CKR-5 抗体 (D-6) Alexa Fluor® 680

sc-17833 AF680
200 µg/ml
RMB2685.00

CKR-5 抗体 (D-6) Alexa Fluor® 790

sc-17833 AF790
200 µg/ml
RMB2685.00

Can it be used for immunohistochemistry?

Asked by: boboo
Thanks for your question. CKR-5 Antibody (D-6) is recommended for detection of CKR-5 of mouse, rat and human origin by IHC on frozen tissue. 感谢您的提问。 CKR-5 抗体(D-6) 推荐检测人，大鼠和小鼠来源的冰冻切片的免疫组化实验。
Answered by: Yanru Tao
Date published: 2023-11-20

What are the exact conditions used to obtain the Western blot data shown on your website for CKR-5 (D-6): sc-17833 monoclonal antibody?

Asked by: cjMara
Thank you for your question. We used this primary antibody at a dilution of 1:200 and incubated for one hour at room temperature in Blotto (sc-2333). The complete recommended protocol for Western blotting is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-01-27
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Rated 5 out of 5 by from 非常好用选择抗体的时候，不必对了几个厂家最后选择这会款，用于检测CCR5，大鼠，wB用，效果挺好的，非常满意，推荐购买。
Date published: 2018-06-18
Rated 1 out of 5 by from anti-CKR5 Ab is not working (very non-specific)Initially, I was having trouble determining the right band for CCR5 on the WB from all the other non-specific bands. Therefore, I decided to run true negative control. I used tzm-bl cell (positive for CCR5 expression) and Hela cells (negative control) to chekc any differences in banding pattern and I do not see any difference. There should not be any CCR5 band in Hela cells.
Date published: 2018-04-12
Rated 4 out of 5 by from Clear with exact positionDiluted 1:500 with casein buffer, samples from ACHN cell line, PVDF memberane with 300mA 120min transfering. It is easy to scan with Odyssey system. The CKR5 is located at about 46 kDa, however, there are two non-specific bands above it.
Date published: 2018-01-26
Rated 3 out of 5 by from some non-specific banduse the monoclone antibody with dilution of 1:500, I can detect the CCR5 band successfully, but there are still some non-specific band on the membrane.
Date published: 2018-01-02
Rated 1 out of 5 by from WBThere was a clear band of WB sample. But its molecular weight (~25 kD) looked smaller than predicted molecular weight (46 kD).
Date published: 2017-09-10
Rated 5 out of 5 by from Highly recommended for WBWwell characterized for WB in human, mouse and rat samples.
Date published: 2016-12-19
Rated 5 out of 5 by from very good antibody for our Western blot asvery good antibody for our Western blot as well as for IP
Date published: 2015-10-21
Rated 5 out of 5 by from No issuesNo issues, very good antibody for our Western.
Date published: 2015-08-13
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CKR-5 抗体 (D-6) is rated 3.8 out of 5 by 13.
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