Date published: 2025-9-7

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CKAP5 抗体 (H-4): sc-374394

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说明书
  • CKAP5 抗体 H-4 是小鼠单克隆 IgG1 κ,CKAP5抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的CKAP5的N-terminus
  • CKAP5 抗体 (H-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CKAP5
  • 抗CKAP5抗体(H-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CKAP5 (H-4): sc-374394免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是CKAP5 Antibody (H-4) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与CKAP5 Antibody (H-4) 打包提供(请参阅下面的订购信息)。

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関連項目

CKAP5抗体(H-4)是一种IgG1 κ型小鼠单克隆CKAP5抗体(也称为CKAP5抗体),可通过WB、IP、IF和ELISA技术检测小鼠、大鼠和人类来源的CKAP5蛋白。CKAP5抗体(H-4)既有非偶联的抗CKAP5抗体形式,也有多种偶联形式的抗CKAP5抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CKAP5(细胞骨架相关蛋白5),也称为ch-TOG(结肠肝肿瘤过表达基因),是一种由2032个氨基酸组成的蛋白,属于TOG/XMAP215家族,含有九个热重复序列。CKAP5在骨骼肌、脑、心脏、胎盘、肺、肝、肾和胰腺中表达,并在肝癌和结肠肿瘤中过表达。在间期,CKAP5定位于核周细胞质和纺锤极,在纺锤体微管组织中发挥重要作用。具体来说,CKAP5对于缺乏Ark-1的早前期中的额外极形成是必需的,Ark-1是一种有丝分裂中心体激酶,作为纺锤体调节因子在肿瘤中过表达。CKAP5与hnRNP A2(异质性核核糖核蛋白)结合,并与TACC1、TACC3和微管蛋白相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CKAP5 抗体 (H-4) 参考文献:

  1. 哺乳动物有丝分裂提取物中微管花束的形成需要ch-TOGp。  |  Dionne, MA., et al. 2000. J Biol Chem. 275: 12346-52. PMID: 10766876
  2. 转化酸性盘卷1(TACC1)蛋白与ch-TOG和GAS41/NuBI1的相互作用表明人体细胞中存在多种含TACC1的蛋白复合物。  |  Lauffart, B., et al. 2002. Biochem J. 363: 195-200. PMID: 11903063
  3. ch-TOG/XMAP215蛋白对人类体细胞的纺锤极组织至关重要。  |  Gergely, F., et al. 2003. Genes Dev. 17: 336-41. PMID: 12569123
  4. 微管相关蛋白肿瘤过表达基因/细胞骨架相关蛋白5是少突胶质细胞中髓鞘碱性蛋白表达所必需的。  |  Francone, VP., et al. 2007. J Neurosci. 27: 7654-62. PMID: 17634360
  5. 整合素连接激酶定位到中心体并调节有丝分裂纺锤体的组织。  |  Fielding, AB., et al. 2008. J Cell Biol. 180: 681-9. PMID: 18283114
  6. Aurora-A和ch-TOG是控制纺锤极完整性的共同途径。  |  De Luca, M., et al. 2008. Oncogene. 27: 6539-49. PMID: 18663358
  7. 一种在人类肝瘤和结肠肿瘤中过度表达的新基因的 cDNA 特征和表达模式。  |  Charrasse, S., et al. 1995. Eur J Biochem. 234: 406-13. PMID: 8536682
  8. 肿瘤过度表达的 ch-TOG 基因在人脑和狒狒脑中的表达。  |  Charrasse, S., et al. 1996. Neurosci Lett. 212: 119-22. PMID: 8832653
  9. TOGp 蛋白是一种新的人类微管相关蛋白,与爪蟾 XMAP215 同源。  |  Charrasse, S., et al. 1998. J Cell Sci. 111 (Pt 10): 1371-83. PMID: 9570755

订购信息

产品名称产品编号规格价格数量收藏夹

CKAP5 抗体 (H-4)

sc-374394
200 µg/ml
RMB2377.00

CKAP5 (H-4): m-IgG Fc BP-HRP 套装

sc-529734
200 µg Ab; 10 µg BP
RMB2662.00

CKAP5 (H-4): m-IgGκ BP-HRP 套装

sc-522720
200 µg Ab, 40 µg BP
RMB2662.00

CKAP5 抗体 (H-4) AC

sc-374394 AC
500 µg/ml, 25% agarose
RMB3129.00

CKAP5 抗体 (H-4) HRP

sc-374394 HRP
200 µg/ml
RMB2377.00

CKAP5 抗体 (H-4) FITC

sc-374394 FITC
200 µg/ml
RMB2482.00

CKAP5 抗体 (H-4) PE

sc-374394 PE
200 µg/ml
RMB2580.00

CKAP5 抗体 (H-4) Alexa Fluor® 488

sc-374394 AF488
200 µg/ml
RMB2685.00

CKAP5 抗体 (H-4) Alexa Fluor® 546

sc-374394 AF546
200 µg/ml
RMB2685.00

CKAP5 抗体 (H-4) Alexa Fluor® 594

sc-374394 AF594
200 µg/ml
RMB2685.00

CKAP5 抗体 (H-4) Alexa Fluor® 647

sc-374394 AF647
200 µg/ml
RMB2685.00

CKAP5 抗体 (H-4) Alexa Fluor® 680

sc-374394 AF680
200 µg/ml
RMB2685.00

CKAP5 抗体 (H-4) Alexa Fluor® 790

sc-374394 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-374394: CKAP5 (H-4) mouse monoclonal antibody will work?

Asked by: jenniferc
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from what a lovely AntibodyI received a sample of CKAP5 antibody and it was great quality. I used it for IHC/Ab-staining and it worked very well.
Date published: 2017-07-18
Rated 5 out of 5 by from WB publishable data with human HCT116 and RPEWB publishable data with human HCT116 and RPE-1-hTert cells - SCBT Publication Review
Date published: 2015-06-30
Rated 5 out of 5 by from Publishable WB data with human HCT116 andPublishable WB data with human HCT116 and isogenic CHK2-deficient cells - SCBT Publication Review
Date published: 2015-05-07
Rated 5 out of 5 by from Good specificity and signal strength in COLO 320DMGood specificity and signal strength in COLO 320DM, HCT 116 and K-562 whole cell lysates. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from Published with this antibody using 1:1000Published with this antibody using 1:1000 for immunoblot analysis -SCBT Publication Review
Date published: 2014-06-29
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CKAP5 抗体 (H-4) is rated 5.0 out of 5 by 5.
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