Date published: 2025-9-8
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CIP2A Antibody (2G10-3B5) - Western Blotting - Image 399130
CIP2A 抗体 (2G10-3B5): sc-80659. K-562 (A), MDA-MB-231 (B), HeLa (C) 和 Jurkat (D) 全细胞裂解液中 CIP2A 表达的 Western 印迹分析. 所用检测试剂: m-IgG2b BP-HRP: sc-542741.
CIP2A 抗体 (2G10-3B5): sc-80659. K-562 (A), MDA-MB-231 (B), HeLa (C) 和 Jurkat (D) 全细胞裂解液中 CIP2A 表达的 Western 印迹分析. 所用检测试剂: m-IgG2b BP-HRP: sc-542741.

CIP2A 抗体 (2G10-3B5): sc-80659

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说明书
  • CIP2A抗体(2G10-3B5)是小鼠单克隆IgG2b κ, 在39篇文献中引用，规格为200 µg/ml
  • 针对C-terminal CIP2A human来源
  • CIP2A 抗体 (2G10-3B5) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 CIP2A
  • 抗CIP2A抗体（2G10-3B5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CIP2A (2G10-3B5): sc-80659的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是CIP2A 抗体 (2G10-3B5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与CIP2A 抗体 (2G10-3B5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CIP2A抗体（2G10-3B5）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测人类CIP2A。CIP2A (2G10-3B5) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。蛋白磷酸酶2A（CIP2A）的癌抑制剂，也称为p90自身抗原或KIAA1524，是一种单通道膜蛋白，通过抑制蛋白磷酸酶2A（PP2A）活性在肿瘤发生中发挥关键作用。PP2A抑制剂的作用非常重要，因为这种重要的肿瘤抑制剂可调节细胞生长和分裂等多种细胞过程。CIP2A通过阻止PP2A对致癌转录因子c-Myc的去磷酸化，稳定c-Myc，从而促进肿瘤形成和恶性细胞增殖。CIP2A与PP2A催化亚基的相互作用对于人类细胞转化进程至关重要，凸显了CIP2A在癌症生物学中的重要性。CIP2A在多种癌症中经常出现过度表达，包括结肠癌、胃癌和头颈部鳞状细胞癌，这凸显了CIP2A作为癌症诊断和预后治疗靶点和生物标志物的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CIP2A 抗体 (2G10-3B5) 参考文献:

  1. 蛋白磷酸酶 2A：与细胞生长和信号传导有关的丝氨酸/苏氨酸磷酸酶高度调节家族。  |  Janssens, V. and Goris, J. 2001. Biochem J. 353: 417-39. PMID: 11171037
  2. 克隆并鉴定癌症中过度表达的 p62 的新型 90 kDa '伴侣 '自身抗原。  |  Soo Hoo, L., et al. 2002. Oncogene. 21: 5006-15. PMID: 12118381
  3. 前列腺癌对一组肿瘤相关抗原中的细胞蛋白 p90 和 p62 的优先体液免疫反应。  |  Shi, FD., et al. 2005. Prostate. 63: 252-8. PMID: 15538718
  4. 蛋白磷酸酶 2A 调节亚基 B56alpha 与 c-myc 缔合作用并负向调节 c-myc 的积累。  |  Arnold, HK. and Sears, RC. 2006. Mol Cell Biol. 26: 2832-44. PMID: 16537924
  5. CIP2A 在人类恶性肿瘤中抑制 PP2A。  |  Junttila, MR., et al. 2007. Cell. 130: 51-62. PMID: 17632056
  6. LINC00665编码的微肽CIP2A-BP可抑制三阴性乳腺癌的进展。  |  Guo, B., et al. 2020. EMBO J. 39: e102190. PMID: 31755573
  7. CIP2A与TopBP1相互作用并驱动基底样乳腺癌的肿瘤发生。  |  Laine, A., et al. 2021. Cancer Res. 81: 4319-4331. PMID: 34145035
  8. CIP2A-TOPBP1 轴保障了染色体的稳定性，是 BRCA 基因突变癌症的合成致死靶点。  |  Adam, S., et al. 2021. Nat Cancer. 2: 1357-1371. PMID: 35121901
  9. CIP2A-TOPBP1 复合物在有丝分裂过程中保护染色体的稳定性。  |  De Marco Zompit, M., et al. 2022. Nat Commun. 13: 4143. PMID: 35842428
  10. m6A修饰介导的lncRNA TP53TG1通过调节CIP2A的稳定性抑制胃癌的进展。  |  Fang, D., et al. 2022. Cancer Sci. 113: 4135-4150. PMID: 36114757
  11. CIP2A缺陷通过抑制树突分支促进小鼠的抑郁样行为。  |  Hu, WT., et al. 2022. EMBO Rep. 23: e54911. PMID: 36305233
  12. CIP2A诱导非小细胞肺癌中PKM2四聚物形成和氧化磷酸化。  |  Liang, LJ., et al. 2024. Cell Discov. 10: 13. PMID: 38321019

订购信息

产品名称产品编号规格价格数量收藏夹

CIP2A 抗体 (2G10-3B5)

sc-80659
200 µg/ml
RMB2377.00

CIP2A (2G10-3B5): m-IgG2b BP-HRP 套装

sc-548661
200 µg Ab; 10 µg BP
RMB2662.00

CIP2A 抗体 (2G10-3B5) AC

sc-80659 AC
500 µg/ml, 25% agarose
RMB3129.00

CIP2A 抗体 (2G10-3B5) HRP

sc-80659 HRP
200 µg/ml
RMB2377.00

CIP2A 抗体 (2G10-3B5) FITC

sc-80659 FITC
200 µg/ml
RMB2482.00

CIP2A 抗体 (2G10-3B5) PE

sc-80659 PE
200 µg/ml
RMB2580.00

CIP2A 抗体 (2G10-3B5) Alexa Fluor® 488

sc-80659 AF488
200 µg/ml
RMB2685.00

CIP2A 抗体 (2G10-3B5) Alexa Fluor® 546

sc-80659 AF546
200 µg/ml
RMB2685.00

CIP2A 抗体 (2G10-3B5) Alexa Fluor® 594

sc-80659 AF594
200 µg/ml
RMB2685.00

CIP2A 抗体 (2G10-3B5) Alexa Fluor® 647

sc-80659 AF647
200 µg/ml
RMB2685.00

CIP2A 抗体 (2G10-3B5) Alexa Fluor® 680

sc-80659 AF680
200 µg/ml
RMB2685.00

CIP2A 抗体 (2G10-3B5) Alexa Fluor® 790

sc-80659 AF790
200 µg/ml
RMB2685.00

The antibody works beautifully on immunofluorescence and immunohistochemistry for cells. However, it does not work on ANY FFPE tissues. We have tried various antigen retrieval approaches but that doesn't seem to work either.

Asked by: Manos
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-06-09

Hello, I would like to ask you for a more detailed description of the conditions for IHC-P with SC-80659 antibody (antigen retrieval, concentration of antibody, incubation time). Well thank you

Asked by: Anonymous
Thank you for your question. For this antibody we recommend a starting dilution of 1:50. We do recommend antigen retrieval with sodium citrate and heat. Incubation time needs to be optimized for your samples, but I would start with an overnight primary incubation, and taper that down if needed. You can find our standard protocol here: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-11-15

Can you recommend a positive control to use with CIP2A (2G10-3B5): sc-80659 monoclonal antibody for Western blot?

Asked by: jenni
We recommend HeLa (sc-2200), SW480 (sc-2219), COLO 320DM (sc-2226) or Caco-2 (sc-2262) whole cell lysates to use as positive controls as we have great Western blot data showing specific detection of CIP2A in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CIP2A (2G10-3B5): sc-80659?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 5 out of 5 by from Post-doctoral researcherExcellent antibody for WB, very strong and specific band with 1:500 dilution, O/n incubation at 4 degrees when used for breast cancer cell lines. Also worked well with mouse cell lysates.
Date published: 2017-11-27
Rated 5 out of 5 by from Great antibody for WBI got the free sample of this antibody and it worked great in WB. The signal is sharp and the background is clear.
Date published: 2017-09-28
Rated 5 out of 5 by from Validated specificity in KO mouseMany commercial CIP2A antibodies are unspecific. Especially in mouse tissues many antibodies crossreact with an unknown 90 kD protein This antibody we have validated to be specific by using lysates from CIP2A KO MEFs (HOZ =KO). This is also the antobody we have found to work best for IHC of human tumor tissues after rigorous testing
Date published: 2017-09-19
Rated 5 out of 5 by from Great WBClear western blot data; strong signal with little or no background
Date published: 2017-01-30
Rated 5 out of 5 by from Good CIP2A detectionConsistent banding in Western blot. Also good for IF and IP.
Date published: 2017-01-27
Rated 5 out of 5 by from good AntibodyApplications used: western blot Cell Line/Specie(s): NSCLC cell lines/human Good Antibody
Date published: 2016-12-26
Rated 5 out of 5 by from Strong band detected at expected molecularStrong band detected at expected molecular weight in HeLa, LS1034, SW480, COLO 320DM and Caco-2 whole cell lysates. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Publishable WB and IF data with human various SKPublishable WB and IF data with human various SK-MEL lines - SCBT Publication Review
Date published: 2015-02-12
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CIP2A抗体 (2G10-3B5) is rated 5.0 out of 5 by 10.
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