Date published: 2025-9-8
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CIITA Antibody (7-1H) - Western Blotting - Image 1456
CIITA 抗体 (7-1H): sc-13556. Western 印迹分析 Jurkat 全细胞裂解液中 CIITA 的表达.
CIITA 抗体 (7-1H): sc-13556. Western 印迹分析 Jurkat 全细胞裂解液中 CIITA 的表达.

CIITA 抗体 (7-1H): sc-13556

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说明书
  • CIITA 抗体 7-1H 是小鼠单克隆 IgG1 κ，CIITA抗体, 在42篇文献中引用，规格为200 µg/ml
  • 针对human来源的纯化重组CIITA的氨基酸1-350
  • CIITA 抗体 (7-1H) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 CIITA
  • 抗CIITA抗体（7-1H）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13556 X, 200 µg/0.1 ml)
  • 关于如何获取CIITA (7-1H): sc-13556的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CIITA 抗体 (7-1H) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CIITA 抗体 (7-1H) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CIITA抗体（7-1H）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测小鼠、大鼠和人类来源的CIITA蛋白。CIITA (7-1H) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。CIITA蛋白在免疫反应中起着至关重要的作用，它作为主要组织相容性复合物（MHC）II类基因表达的主调节器，对于向CD4+ T细胞呈递抗原至关重要。这一过程对于激活适应性免疫至关重要，因为CD4+ T细胞能够识别并应对病原体。CIITA在免疫系统发育中的功能尤为重要，因为CIITA的缺陷会导致严重的免疫缺陷，例如裸淋巴细胞综合征，其特征是细胞表面没有MHC II类分子。CIITA的表达由γ干扰素通过JAK1和Stat1信号通路诱导，突出了其在感染免疫反应中的作用。CIITA与多种蛋白相互作用，包括RFX-B，后者是MHC II类转录所需的RFX复合物的一部分，进一步强调了免疫反应的复杂调节机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CIITA 抗体 (7-1H) 参考文献:

  1. 分析 G1B 细胞中 IFN-gamma 诱导 MHC II 类基因的缺陷：鉴定调节因子 X 5 转录因子中一个新的功能关键富亮氨酸基序（62-LYLYLQL-68）。  |  Brickey, WJ., et al. 1999. J Immunol. 163: 6622-30. PMID: 10586057
  2. 全基因组 CIITA 结合概况确定了决定功能与招募的序列偏好。  |  Scharer, CD., et al. 2015. Nucleic Acids Res. 43: 3128-42. PMID: 25753668
  3. FBXO11 通过泛素依赖的蛋白酶体降解 CIITA，构成了 MHC II 类的主要负调控因子。  |  Kasuga, Y., et al. 2023. Proc Natl Acad Sci U S A. 120: e2218955120. PMID: 37279268
  4. NLR 成员 CIITA：适应性免疫和内在免疫的主控器以及癌症免疫疗法中意想不到的工具。  |  Forlani, G., et al. 2023. Biomed J. 46: 100631. PMID: 37467968
  5. LncRNA-HOXC-AS2通过STAT1/SOCS1和STAT1/CIITA途径调控肿瘤相关巨噬细胞的极化，从而促进非小细胞肺癌的进展。  |  Yin, C., et al. 2024. Cell Signal. 115: 111031. PMID: 38168631
  6. 对 G1B 和 G3A IFN gamma 突变体的分子分析表明，CIITA 或 RFX 的缺陷会导致 II 类 MHC 和 Ii 基因诱导缺陷。  |  Chin, KC., et al. 1994. Immunity. 1: 687-97. PMID: 7600294
  7. 对遗传性 MHC II 类缺乏症（或裸淋巴细胞综合征）中突变的 MHC II 类转座子进行补体克隆。  |  Steimle, V., et al. 1993. Cell. 75: 135-46. PMID: 8402893
  8. 调节 MHC II 类基因的转录。  |  Boss, JM. 1997. Curr Opin Immunol. 9: 107-13. PMID: 9039770
  9. II 型裸淋巴细胞综合征相关转录因子 CIITA 和 RFX5 之间形成特异性复合物。  |  Scholl, T., et al. 1997. Proc Natl Acad Sci U S A. 94: 6330-4. PMID: 9177217
  10. 调节因子 X 是一种裸淋巴细胞综合征转录因子，是一种多聚磷蛋白复合物。  |  Moreno, CS., et al. 1997. J Immunol. 158: 5841-8. PMID: 9190936
  11. 在大多数 MHC II 类缺乏症患者中，一种编码新型 RFX 相关转座子的基因发生了突变。  |  Masternak, K., et al. 1998. Nat Genet. 20: 273-7. PMID: 9806546
  12. 滋养层细胞中的 MHC II 类基因沉默是由 CIITA 表达抑制引起的。  |  Morris, AC., et al. 1998. Am J Reprod Immunol. 40: 385-94. PMID: 9894561

订购信息

产品名称产品编号规格价格数量收藏夹

CIITA 抗体 (7-1H)

sc-13556
200 µg/ml
RMB2377.00

CIITA (7-1H): m-IgG Fc BP-HRP 套装

sc-526559
200 µg Ab; 10 µg BP
RMB2662.00

CIITA (7-1H): m-IgG1 BP-HRP 套装

sc-531932
200 µg Ab; 20 µg BP
RMB2662.00

CIITA 抗体 (7-1H) X

sc-13556 X
200 µg/0.1 ml
RMB2377.00

CIITA 抗体 (7-1H) AC

sc-13556 AC
500 µg/ml, 25% agarose
RMB3129.00

CIITA 抗体 (7-1H) HRP

sc-13556 HRP
200 µg/ml
RMB2377.00

CIITA 抗体 (7-1H) FITC

sc-13556 FITC
200 µg/ml
RMB2482.00

CIITA 抗体 (7-1H) PE

sc-13556 PE
200 µg/ml
RMB2580.00

CIITA 抗体 (7-1H) Alexa Fluor® 488

sc-13556 AF488
200 µg/ml
RMB2685.00

CIITA 抗体 (7-1H) Alexa Fluor® 546

sc-13556 AF546
200 µg/ml
RMB2685.00

CIITA 抗体 (7-1H) Alexa Fluor® 594

sc-13556 AF594
200 µg/ml
RMB2685.00

CIITA 抗体 (7-1H) Alexa Fluor® 647

sc-13556 AF647
200 µg/ml
RMB2685.00

CIITA 抗体 (7-1H) Alexa Fluor® 680

sc-13556 AF680
200 µg/ml
RMB2685.00

CIITA 抗体 (7-1H) Alexa Fluor® 790

sc-13556 AF790
200 µg/ml
RMB2685.00

Dear all,  I am interested in the CIITA antibody (7-1H). Thus, I have two questions regarding this product. Is it possible to get this antibody carrier-free (no BSA, no azide)?And could we get a small samples to test it?  Best, 

Asked by: Domenico
Thank you for your question. Please contact our technical service team directly for information on custom formulation versions of this product and availability of sample sizes.
Answered by: Tech Support Europe
Date published: 2024-06-19

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-11
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Rated 5 out of 5 by from 可以用于绵羊CIITA采用CIITA抗体 (7-1H)检测绵羊子宫内膜CIITA蛋白表达，条带清晰，推荐使用该抗体做WB。
Date published: 2024-10-26
Rated 5 out of 5 by from Ottimo anticorpoVery good antibody for IHC on FFPE, I’ve achieved a good and clean signal in lymph nodes and colic mucosa. The tissues were subjected to antigen retrieval using water bath at 98°C for 40 mins in EDTA pH8.0, the antibody diluted 1:50 was incubated 1h RT and Novolink polymer (Leica Biosystems) was used as revelation kit. DAB was used as chromogen, stainings using AP chromogens like Ferangi Blue or Vector Blue give also a bright signal.
Date published: 2021-06-14
Rated 5 out of 5 by from perfect signalThis CIITA antibody is really good for WB, robust band at 1:500 dilution
Date published: 2017-04-22
Rated 4 out of 5 by from WBI have got a dim band at 130kd in human macrophage lysate
Date published: 2017-04-13
Rated 5 out of 5 by from Superb western and IHCStrong, clear western blot data; also great IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Good antibodyThe CIITA Antibody (7-1H): sc-13556 provides excellent Western blot data showing clear band with minimal background.
Date published: 2017-01-12
Rated 4 out of 5 by from very good antibodyI use for both WB and IP and it works very good . happy with thew product.
Date published: 2017-01-04
Rated 5 out of 5 by from We used this antibody in WB and IP got strong bandWe used this antibody in WB and IP got strong band, no complaints.
Date published: 2015-10-07
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CIITA 抗体 (7-1H) is rated 4.9 out of 5 by 14.
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