Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
ChREBP Antibody (G-12) - Western Blotting - Image 377907
ChREBP 抗体 (G-12): sc-515922. Hep G2 (A) 和 K-562 (B) 核提取物中 ChREBP 表达的 Western 印迹分析.
ChREBP Antibody (G-12) - Western Blotting - Image 377907
ChREBP 抗体 (G-12): sc-515922. Hep G2 (A) 和 K-562 (B) 核提取物中 ChREBP 表达的 Western 印迹分析.
ChREBP Antibody (G-12) - Western Blotting - Image 385691
ChREBP 抗体 (G-12): sc-515922. K-562 核提取物中 ChREBP 表达的 Western 印迹分析.
ChREBP 抗体 (G-12): sc-515922. Hep G2 (A) 和 K-562 (B) 核提取物中 ChREBP 表达的 Western 印迹分析.

ChREBP 抗体 (G-12): sc-515922

4.2(6)
寫評論提問

说明书
  • ChREBP 抗体 G-12 是小鼠单克隆 IgG2a κ，ChREBP抗体, 在8篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的的C-terminus附近的氨基酸817-838之间
  • ChREBP 抗体 (G-12) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 ChREBP
  • 抗ChREBP抗体（G-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ChREBP (G-12): sc-515922的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是ChREBP Antibody (G-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ChREBP Antibody (G-12) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

ChREBP抗体（G-12）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测人类来源的ChREBP蛋白。抗 REBP抗体（G-12）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。ChREBP，也称为碳水化合物反应元件结合蛋白，作为转录因子在调节葡萄糖代谢方面发挥着至关重要的作用，它通过与L型丙酮酸激酶基因（L-PK）的碳水化合物反应元件结合。ChREBP主要在肝脏中表达，并在高血糖水平下激活，而环磷酸腺苷或高脂饮食会抑制ChREBP的活性。ChREBP的功能对于将多余的碳水化合物长期储存为脂肪至关重要，这在代谢健康和肥胖方面尤为重要，因为营养利用和储存失衡会导致各种健康问题。ChREBP与Mlx相互作用形成异二聚体，与特定的DNA序列结合，从而影响基因表达。ChREBP由WBSCR14基因编码，该基因位于与威廉姆斯-博伊伦综合征相关的缺失区域中，威廉姆斯-博伊伦综合征是一种神经发育障碍，由于这种转录因子的缺失，会导致发育症状。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ChREBP 参考文献:

  1. WBSCR14，一个在威廉姆斯-伯恩综合征中缺失的假定转录因子基因：人类基因和小鼠同源物的完整特征。  |  de Luis, O., et al. 2000. Eur J Hum Genet. 8: 215-22. PMID: 10780788
  2. WBSCR14是威廉姆斯--博伊伦综合征删除区的一个基因，它是Mlx转录因子网络的一个新成员。  |  Cairo, S., et al. 2001. Hum Mol Genet. 10: 617-27. PMID: 11230181
  3. 一种葡萄糖反应转录因子，可调节肝脏中的碳水化合物代谢。  |  Yamashita, H., et al. 2001. Proc Natl Acad Sci U S A. 98: 9116-21. PMID: 11470916
  4. 葡萄糖和 cAMP 通过碳水化合物反应元件结合蛋白的磷酸化/去磷酸化调节 L 型丙酮酸激酶基因。  |  Kawaguchi, T., et al. 2001. Proc Natl Acad Sci U S A. 98: 13710-5. PMID: 11698644
  5. 脂肪酸对葡萄糖诱导转录的 '节约 '效应机制：AMP 激活蛋白激酶对碳水化合物反应元件结合蛋白的调控。  |  Kawaguchi, T., et al. 2002. J Biol Chem. 277: 3829-35. PMID: 11724780
  6. 通过转录因子ChREBP进行碳水化合物感应。  |  Ortega-Prieto, P. and Postic, C. 2019. Front Genet. 10: 472. PMID: 31275349
  7. ChREBP介导的脂质代谢调节：肠道菌群、肝脏和脂肪组织的参与。  |  Iizuka, K., et al. 2020. Front Endocrinol (Lausanne). 11: 587189. PMID: 33343508
  8. 抑制肝细胞Kctd17可改善肥胖引起的葡萄糖耐受不良和脂肪变性。  |  Oh, AR., et al. 2023. Gastroenterology. 164: 439-453. PMID: 36402191
  9. ChREBP在碳水化合物感知和NAFLD发展中的作用。  |  Régnier, M., et al. 2023. Nat Rev Endocrinol. 19: 336-349. PMID: 37055547
  10. 代谢性疾病中MondoA和ChREBP营养感应因子的功能。  |  Ahn, B. 2023. Int J Mol Sci. 24: PMID: 37240157
  11. ChREBP受还原应激激活，并介导GCKR相关的代谢特征。  |  Singh, C., et al. 2024. Cell Metab. 36: 144-158.e7. PMID: 38101397
  12. 转录因子ChREBP通过协调PI3K/AKT信号传导和癌症代谢来协调肝脏癌变。  |  Benichou, E., et al. 2024. Nat Commun. 15: 1879. PMID: 38424041

订购信息

产品名称产品编号规格价格数量收藏夹

ChREBP 抗体 (G-12)

sc-515922
200 µg/ml
RMB2377.00

ChREBP (G-12): m-IgGκ BP-HRP 套装

sc-525284
200 µg Ab, 40 µg BP
RMB2662.00

ChREBP (G-12): m-IgG2a BP-HRP 套装

sc-546180
200 µg Ab; 10 µg BP
RMB2662.00

ChREBP 抗体 (G-12) AC

sc-515922 AC
500 µg/ml, 25% agarose
RMB3129.00

ChREBP 抗体 (G-12) HRP

sc-515922 HRP
200 µg/ml
RMB2377.00

ChREBP 抗体 (G-12) FITC

sc-515922 FITC
200 µg/ml
RMB2482.00

ChREBP 抗体 (G-12) PE

sc-515922 PE
200 µg/ml
RMB2580.00

ChREBP 抗体 (G-12) Alexa Fluor® 488

sc-515922 AF488
200 µg/ml
RMB2685.00

ChREBP 抗体 (G-12) Alexa Fluor® 546

sc-515922 AF546
200 µg/ml
RMB2685.00

ChREBP 抗体 (G-12) Alexa Fluor® 594

sc-515922 AF594
200 µg/ml
RMB2685.00

ChREBP 抗体 (G-12) Alexa Fluor® 647

sc-515922 AF647
200 µg/ml
RMB2685.00

ChREBP 抗体 (G-12) Alexa Fluor® 680

sc-515922 AF680
200 µg/ml
RMB2685.00

ChREBP 抗体 (G-12) Alexa Fluor® 790

sc-515922 AF790
200 µg/ml
RMB2685.00

Is this antibody only able to detect human ChREBP? not rat, or mouse? Can it be used for ChIP assay? We have used p13 antibody many years for ChIP assay in rat INS-1 beta cells. It works so great! Surprise, it was stopped!

Asked by: Gu Jing
Thank you for your question. This antibody, sc-515922 ChREBP (G-12) is only recommended for use on human samples, it has not been tested for mouse or rat samples, and can not be warrantied for that use. We do not offer a mouse reactive ChREBP antibody at this time. Unfortunately, our P-13 clone was discontinued a few months ago when we switched to being a monoclonal specific company. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-05-30
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_515922, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from 功能出色，减少了我实验试错的时间。我前几周购买的产品，物流和包装都很满意，而且产品质量没得说。确实很好用。是一次开心的购物经历，会再次购买的
Date published: 2022-04-02
Rated 4 out of 5 by from goodI bought this antibody one month ago and it is specific to produce good western blottings.
Date published: 2022-01-20
Rated 5 out of 5 by from Clean bands in bovine samplesWe bought this antibody to quantify ChREBP in bovine samples and were pleased with the results. A 100 kDa band appears when diluted at 1:250 in 5% milk in TBST when incubated at 4C overnight. An added bonus is that it is monoclonal, so when the splice variant shows up, we know it is not nonspecific binding.
Date published: 2021-06-22
Rated 5 out of 5 by from ChREBP antibody for human proteinThis antibody gave a clean band with little background on a nitrocellulose Western blot. We used it at 1:2000 in 4% BSA for 3 hours at RT.
Date published: 2019-11-01
Rated 2 out of 5 by from Maybe not great for 293T cellsI have yet to see any bands when I have used this antibody for Western blotting. I am using 293T cells, and perhaps expression is low in this cell type (people usually over-express this protein in 293T). I will switch to a beta-cell or liver cell type, where expression should be higher and try again.
Date published: 2017-09-28
Rated 4 out of 5 by from GOODThank you for your sample. The sc-515922_sample,ChREBP Antibody (G-12)_sample is used for WB, the effect is very obvious.
Date published: 2017-09-15
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_515922, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
ChREBP 抗体 (G-12) is rated 4.2 out of 5 by 6.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_515922, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。