Date published: 2025-9-7
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CHMP5 Antibody (F-7) - Western Blotting - Image 150026
CHMP5 抗体 (F-7): sc-374338. K-562 (A) 和 SK-N-SH (B) 全细胞裂解液中 CHMP5 表达的 Western 印迹分析.
CHMP5 Antibody (F-7) - Western Blotting - Image 150026
CHMP5 抗体 (F-7): sc-374338. K-562 (A) 和 SK-N-SH (B) 全细胞裂解液中 CHMP5 表达的 Western 印迹分析.
CHMP5 Antibody (F-7) - Immunofluorescence - Image 149276
CHMP5 抗体 (F-7): sc-374338. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞质定位.
CHMP5 Antibody (F-7) - Western Blotting - Image 365603
CHMP5 抗体 (F-7): sc-374338. K-562 (A), A549 (B) 和 SW480 (C) 全细胞裂解液中 CHMP5 表达的 Western 印迹分析.
CHMP5 抗体 (F-7): sc-374338. K-562 (A) 和 SK-N-SH (B) 全细胞裂解液中 CHMP5 表达的 Western 印迹分析.

CHMP5 抗体 (F-7): sc-374338

5.0(7)
寫評論提問

说明书
  • CHMP5 抗体 F-7 是小鼠单克隆 IgG2b κ，CHMP5抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列130-219位于human物种的CHMP5的C-terminus
  • CHMP5 抗体 (F-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CHMP5; 也和以下物种反应，包括： 和 bovine and equine
  • 抗CHMP5抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CHMP5 (F-7): sc-374338的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是CHMP5 Antibody (F-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CHMP5 Antibody (F-7) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外CHMP抗体，包括CHMP1A, CHMP1B, CHMP3, CHMP4A, CHMP5, CHMP6和CHMP7

CHMP5抗体（F-7）是一种IgG2b κ鼠单克隆CHMP5抗体（也称为CHMP5抗体），可通过WB、IP、IF和ELISA检测到小鼠、大鼠和人的CHMP5蛋白。CHMP5抗体（F-7）既可以是非偶联的抗CHMP5抗体形式，也可以是多种偶联形式的抗CHMP5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。带电的多泡体蛋白，通常称为CHMPs，属于液泡分选蛋白家族，并具有染色质修饰蛋白的功能。CHMP1-6都是ESCRT（运输所需的内体分选复合物）I、II或III复合物的组成部分。这些复合物对于将内体物质分选到多泡体（MVBs）中至关重要，并且是这些体形成所必需的。在HIV-1感染期间，病毒利用ESCRT-III复合物介导病毒蛋白的出芽和外排。CHMP5直接与LIP5相互作用，LIP5是HIV释放所需的蛋白质。LIP5的耗竭将减少HIV-1的出芽，而CHMP5的耗竭将增加HIV-1的释放。随后，CHMP5的过度表达将减少HIV-1的出芽。CHMP5还在MVB形成下游调节晚期内体发育，CHMP5的缺失将导致信号转导增加，这是由于溶酶体降解功能降低所致。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CHMP5 抗体 (F-7) 参考文献:

  1. 艾滋病毒萌芽的蛋白质网络  |  von Schwedler, UK., et al. 2003. Cell. 114: 701-13. PMID: 14505570
  2. LIP5和CHMP5在哺乳动物细胞多囊体形成和HIV-1萌发中的作用  |  Ward, DM., et al. 2005. J Biol Chem. 280: 10548-55. PMID: 15644320
  3. 在小鼠胚胎发育过程中，CHMP5对晚期内质体功能和受体信号的下调至关重要。  |  Shim, JH., et al. 2006. J Cell Biol. 172: 1045-56. PMID: 16567502

订购信息

产品名称产品编号规格价格数量收藏夹

CHMP5 抗体 (F-7)

sc-374338
200 µg/ml
RMB2377.00

CHMP5 (F-7): m-IgGκ BP-HRP 套装

sc-522704
200 µg Ab, 40 µg BP
RMB2662.00

CHMP5 (F-7): m-IgG2b BP-HRP 套装

sc-549058
200 µg Ab; 10 µg BP
RMB2662.00

CHMP5 抗体 (F-7) AC

sc-374338 AC
500 µg/ml, 25% agarose
RMB3129.00

CHMP5 抗体 (F-7) HRP

sc-374338 HRP
200 µg/ml
RMB2377.00

CHMP5 抗体 (F-7) FITC

sc-374338 FITC
200 µg/ml
RMB2482.00

CHMP5 抗体 (F-7) PE

sc-374338 PE
200 µg/ml
RMB2580.00

CHMP5 抗体 (F-7) Alexa Fluor® 488

sc-374338 AF488
200 µg/ml
RMB2685.00

CHMP5 抗体 (F-7) Alexa Fluor® 546

sc-374338 AF546
200 µg/ml
RMB2685.00

CHMP5 抗体 (F-7) Alexa Fluor® 594

sc-374338 AF594
200 µg/ml
RMB2685.00

CHMP5 抗体 (F-7) Alexa Fluor® 647

sc-374338 AF647
200 µg/ml
RMB2685.00

CHMP5 抗体 (F-7) Alexa Fluor® 680

sc-374338 AF680
200 µg/ml
RMB2685.00

CHMP5 抗体 (F-7) Alexa Fluor® 790

sc-374338 AF790
200 µg/ml
RMB2685.00

I had booked a antibody chmp5 F-7（374338），but it always has blank blot for the extracted prostate cell（lysates 40 ul）。the dilution is 1：100，RT,overnight .I donot know why 。please give me a hand。thank you so much.

Asked by: Anonymous
Thank you for your question. There are a lot of different factors that may cause a blot to come up blank. Please reach out to our technical service team so we can assist you with your experiment.
Answered by: Technical Support Europe
Date published: 2020-10-14

What is the recommended fixation method for immunofluorescence staining with CHMP5 (F-7): sc-374338 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works very well for western blotting (MCF7 cells)I used this antibody for western blotting on MCF7 cell extracts (dilution 1:1000, TBST-5%milk). It produces a clear band at the described size, using normal ECL reagent.
Date published: 2020-02-14
Rated 5 out of 5 by from Good antibody for co-IP, IFA, and Western blotI buy this antibody several times. In addition to the discontinued rabbit pAb against CHMP5 (sc-67230), this mouse antibody is an excellent antibody applicable to co-IP, IFA, Western blot and to be validated in CHMP5 knockdown assays. Similar results are also obtained in another CHMP5 antibody (sc-374337); but sc-374338 could be diluted more than sci-374337. (1: 5000 vs. 1: 1000)
Date published: 2018-08-09
Rated 5 out of 5 by from purchase it againShows good detection in WB, this antibody works good at 1:1000 dilution
Date published: 2017-09-07
Rated 5 out of 5 by from works very wellWe use it often , works very well in WB at 1:500 dilution.
Date published: 2017-05-28
Rated 5 out of 5 by from CHMP5 AntibodyCHMP5 Antibody (F-7) (sc-374338) at 1/1000 dilution, lysates/proteins at 30 µg per lane, get a clear band 25 kDa.
Date published: 2017-01-05
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Great Western Blot data of CHMP5 expression in KGreat Western Blot data of CHMP5 expression in K-562 and SK-N-SH whole cell lysates. -SCBT QC
Date published: 2014-03-17
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CHMP5 抗体 (F-7) is rated 5.0 out of 5 by 7.
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