Date published: 2025-9-8
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CHIP/STUB1 Antibody (C-10) - Western Blotting - Image 362036
CHIP 抗体 (C-10): sc-133083. HeLa (A), HEK293 (B), PC-12 (C), Neuro-2A (D), NIH/3T3 (E) 和 C3H/10T1/2 (F) 全细胞裂解液中 CHIP 表达的 Western 印迹分析.
CHIP 抗体 (C-10): sc-133083. HeLa (A), HEK293 (B), PC-12 (C), Neuro-2A (D), NIH/3T3 (E) 和 C3H/10T1/2 (F) 全细胞裂解液中 CHIP 表达的 Western 印迹分析.

CHIP/STUB1 抗体 (C-10): sc-133083

4.6(7)
寫評論提問

说明书
  • CHIP/STUB1抗体(C-10)是小鼠单克隆IgG1 κ, 在16篇文献中引用，规格为200 µg/ml
  • 针对氨基酸73-303，在CHIP的human起源的C-terminus处进行映射
  • 抗-CHIP/STUB1 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CHIP
  • 抗CHIP/STUB1抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CHIP/STUB1 (C-10): sc-133083的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CHIP/STUB1 Antibody (C-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CHIP/STUB1 Antibody (C-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CHIP/STUB1抗体（C-10）是一种IgG1κ小鼠单克隆CHIP抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源CHIP。CHIP/STUB1抗体（C-10）可作为非偶联抗CHIP抗体形式，以及多种偶联形式的抗CHIP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CHIP（HSP70相互作用蛋白的羧基末端），也称为STIP1同源和含U-box的蛋白1、HSPABP2、NY-CO-7、SDCCAG7和STUB1，是一种影响蛋白质泛素化的细胞质E3泛素连接酶。CHIP与Smad1/Smad4相互作用，并通过泛素介导的Smad蛋白降解阻断BMP信号传导。CHIP控制Hsp70/Hsp90伴侣与ErbB2的结合以及Hsp90抑制剂诱导的ErbB2下调。一条1.3kb的转录本在横纹肌（心脏和骨骼肌）中最为丰富，在胰腺和脑中的表达较低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CHIP/STUB1 抗体 (C-10)

sc-133083
200 µg/ml
RMB2377.00

CHIP/STUB1 (C-10): m-IgG Fc BP-HRP 套装

sc-528743
200 µg Ab; 10 µg BP
RMB2662.00

CHIP/STUB1 (C-10): m-IgGκ BP-HRP 套装

sc-521226
200 µg Ab, 40 µg BP
RMB2662.00

CHIP/STUB1 (C-10): m-IgG1 BP-HRP 套装

sc-543107
200 µg Ab; 20 µg BP
RMB2662.00

CHIP/STUB1 抗体 (C-10) AC

sc-133083 AC
500 µg/ml, 25% agarose
RMB3129.00

CHIP/STUB1 抗体 (C-10) HRP

sc-133083 HRP
200 µg/ml
RMB2377.00

CHIP/STUB1 抗体 (C-10) FITC

sc-133083 FITC
200 µg/ml
RMB2482.00

CHIP/STUB1 抗体 (C-10) PE

sc-133083 PE
200 µg/ml
RMB2580.00

CHIP/STUB1 抗体 (C-10) Alexa Fluor® 488

sc-133083 AF488
200 µg/ml
RMB2685.00

CHIP/STUB1 抗体 (C-10) Alexa Fluor® 546

sc-133083 AF546
200 µg/ml
RMB2685.00

CHIP/STUB1 抗体 (C-10) Alexa Fluor® 594

sc-133083 AF594
200 µg/ml
RMB2685.00

CHIP/STUB1 抗体 (C-10) Alexa Fluor® 647

sc-133083 AF647
200 µg/ml
RMB2685.00

CHIP/STUB1 抗体 (C-10) Alexa Fluor® 680

sc-133083 AF680
200 µg/ml
RMB2685.00

CHIP/STUB1 抗体 (C-10) Alexa Fluor® 790

sc-133083 AF790
200 µg/ml
RMB2685.00

How much sample do you recommend loading on the gel for Western blot detection using CHIP (C-10): sc-133083?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2018-03-26
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Rated 5 out of 5 by from Detects endogenous hsCHIP in HEK293T cells in WBThe antibody works in a dilution of 1:500 in WB. I loaded 40 ug cell lysate of HEK293T cells. Only one band is detected around 35 kD.
Date published: 2019-01-29
Rated 5 out of 5 by from wonderful antibody!1:200 diluted for wb of rat protein ,the band is quite strong
Date published: 2018-06-12
Rated 5 out of 5 by from Great CHIP antibody!Excellent antibody choice for detecting CHIP in Western Blot, shows a clean strong band with no background or additional bands.
Date published: 2017-01-26
Rated 4 out of 5 by from Positive results in human CHIP transfectedPositive results in human CHIP transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-17
Rated 4 out of 5 by from No commentNo comment
Date published: 2015-03-31
Rated 5 out of 5 by from Published IF data using embryonic stem cellsPublished IF data using embryonic stem cells (ESCs) & embryoid bodies (EB). -SCBT Publication Review
Date published: 2015-03-12
Rated 4 out of 5 by from Used for western blotting with murine heartUsed for western blotting with murine heart extracts. -SCBT Publication Review
Date published: 2015-02-23
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CHIP/STUB1 抗体 (C-10) is rated 4.6 out of 5 by 7.
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