Date published: 2025-9-6

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Ceramide Kinase 抗体 (G-3): sc-376730

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说明书
  • Ceramide Kinase 抗体 G-3 是小鼠单克隆 IgG2b κ,Ceramide Kinase抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列238-474位于human物种的Ceramide Kinase的C-terminus附近
  • Ceramide Kinase 抗体 (G-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ceramide Kinase
  • 抗Ceramide Kinase抗体(G-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ceramide Kinase (G-3): sc-376730免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是Ceramide Kinase Antibody (G-3) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与Ceramide Kinase Antibody (G-3) 打包提供(请参阅下面的订购信息)。

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関連項目

Ceramide Kinase抗体(G-3)是一种IgG2b κ小鼠单克隆Ceramide Kinase抗体(也称为Ceramide Kinase抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人的Ceramide Kinase蛋白。Ceramide Kinase抗体(G-3)既可以是非偶联型抗Ceramide Kinase抗体形式,也可以是多种偶联型抗Ceramide Kinase抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ceramide Kinase,也称为CERK、LK4(脂质激酶4)或酰基鞘氨醇激酶,是一种由537个氨基酸组成的外周膜蛋白,定位于膜和细胞质,并含有一个DAGHc结构域。Ceramide Kinase在脑、肝、肾、心脏和骨骼肌中高表达,在脾、肺、胸腺和小肠中表达较低,它使用钙和镁作为辅因子,催化ATP依赖性的Ceramide向Ceramide 1-phosphate(C1P)的转化,这是一种鞘脂代谢产物。Ceramide Kinase在pH值为6-7.5时发挥最佳功能,并通过其催化活性在多种细胞过程中发挥重要作用,包括凋亡、吞噬和细胞增殖。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ceramide Kinase 抗体 (G-3) 参考文献:

  1. 神经酰胺激酶,一种新型脂质激酶。分子克隆和功能表征。  |  Sugiura, M., et al. 2002. J Biol Chem. 277: 23294-300. PMID: 11956206
  2. 神经酰胺 1-磷酸,一种吞噬介质。  |  Hinkovska-Galcheva, V., et al. 2005. J Biol Chem. 280: 26612-21. PMID: 15899891
  3. 人类神经酰胺激酶的底物特异性。  |  Wijesinghe, DS., et al. 2005. J Lipid Res. 46: 2706-16. PMID: 16170208
  4. 哺乳动物神经酰胺激酶的进一步表征:底物输送和(立体)特异性, 组织分布和亚细胞定位研究。  |  Van Overloop, H., et al. 2006. J Lipid Res. 47: 268-83. PMID: 16269826
  5. 神经酰胺激酶调节 A549 人肺腺癌细胞的生长和存活。  |  Mitra, P., et al. 2007. FEBS Lett. 581: 735-40. PMID: 17274985
  6. 神经酰胺激酶利用神经酰胺转运蛋白提供的神经酰胺:定域到二十烷合成的细胞器。  |  Lamour, NF., et al. 2007. J Lipid Res. 48: 1293-304. PMID: 17392267
  7. 神经酰胺激酶和神经酰胺-1-磷酸。  |  Wijesinghe, DS., et al. 2007. Methods Enzymol. 434: 265-92. PMID: 17954253
  8. 神经酰胺激酶的表达在白血病细胞株 HL-60 的巨噬细胞样分化过程中发生改变。  |  Date, T., et al. 2007. In Vitro Cell Dev Biol Anim. 43: 321-3. PMID: 17957442
  9. 神经酰胺激酶可促进 IgG 冲洗目标附近的 Ca2+ 信号传导,并增强 COS-1 细胞中吞噬溶酶体的融合。  |  Hinkovska-Galcheva, V., et al. 2008. J Lipid Res. 49: 531-42. PMID: 18156590
  10. 神经酰胺激酶/C1P在癌症发生和进展中的作用。  |  Camacho, L., et al. 2022. Cancers (Basel). 14: PMID: 35008391
  11. 神经酰胺激酶抑制通过失调 VDAC 介导的线粒体功能驱动突变 KRAS 肺癌中的铁变态反应和对顺铂的敏感性  |  Vu, NT., et al. 2022. Mol Cancer Res. 20: 1429-1442. PMID: 35560154
  12. 神经酰胺激酶介导的C1P代谢通过抑制KEAP1和NRF2之间的相互作用减轻急性肝损伤。  |  Dong, W., et al. 2024. Exp Mol Med. 56: 946-958. PMID: 38556546

订购信息

产品名称产品编号规格价格数量收藏夹

Ceramide Kinase 抗体 (G-3)

sc-376730
200 µg/ml
RMB2377.00

Ceramide Kinase (G-3): m-IgG Fc BP-HRP 套装

sc-526045
200 µg Ab; 10 µg BP
RMB2662.00

Ceramide Kinase (G-3): m-IgG2b BP-HRP 套装

sc-549667
200 µg Ab; 10 µg BP
RMB2662.00

Ceramide Kinase 抗体 (G-3) AC

sc-376730 AC
500 µg/ml, 25% agarose
RMB3129.00

Ceramide Kinase 抗体 (G-3) HRP

sc-376730 HRP
200 µg/ml
RMB2377.00

Ceramide Kinase 抗体 (G-3) FITC

sc-376730 FITC
200 µg/ml
RMB2482.00

Ceramide Kinase 抗体 (G-3) PE

sc-376730 PE
200 µg/ml
RMB2580.00

Ceramide Kinase 抗体 (G-3) Alexa Fluor® 488

sc-376730 AF488
200 µg/ml
RMB2685.00

Ceramide Kinase 抗体 (G-3) Alexa Fluor® 546

sc-376730 AF546
200 µg/ml
RMB2685.00

Ceramide Kinase 抗体 (G-3) Alexa Fluor® 594

sc-376730 AF594
200 µg/ml
RMB2685.00

Ceramide Kinase 抗体 (G-3) Alexa Fluor® 647

sc-376730 AF647
200 µg/ml
RMB2685.00

Ceramide Kinase 抗体 (G-3) Alexa Fluor® 680

sc-376730 AF680
200 µg/ml
RMB2685.00

Ceramide Kinase 抗体 (G-3) Alexa Fluor® 790

sc-376730 AF790
200 µg/ml
RMB2685.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Ceramide Kinase (G-3): sc-376730?

Asked by: DefinitelyNotMatt
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Scarso funzionamentoHo richiesto la small size qualche settimana fa e lo abbiamo usato su estratti totali di cellule SiHa, C4I e K562. Si rileva la presenza di numerose bande di altezze diverse con aspetto di aspecificità.
Date published: 2023-06-26
Rated 1 out of 5 by from Multiple non-specific bands.I got a sample antibody, but unfortunately it couldn't detect CERK at the expected molecular weight, instead I got a blot full of non-specific bands. I don't recommend this antibody.
Date published: 2017-09-18
Rated 1 out of 5 by from En pollo no funcionaEn Western Blotting de muestras de pollo da un patrón de bandas inespecífico que abarca todo el rango de pesos moleculares.
Date published: 2017-06-05
Rated 5 out of 5 by from Good Western blot data of Ceramide KinaseGood Western blot data of Ceramide Kinase expression in non-transfected and human Ceramide Kinase transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-05
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Ceramide Kinase 抗体 (G-3) is rated 2.0 out of 5 by 4.
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