Date published: 2025-9-7

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Centriolin 抗体 (C-9): sc-365521

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说明书
  • Centriolin 抗体 C-9 是小鼠单克隆 IgG1 κ,Centriolin抗体, 在18篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列2026-2325位于human物种的Centriolin的C-terminus
  • Centriolin 抗体 (C-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Centriolin
  • 抗Centriolin抗体(C-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Centriolin (C-9): sc-365521免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Centriolin Antibody (C-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Centriolin Antibody (C-9) 打包提供(请参阅下面的订购信息)。

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関連項目

Centriolin抗体(C-9)是一种IgG1 κ型小鼠单克隆Centriolin抗体(也称为Centriolin抗体),能够通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学(IHC,P)和酶联免疫吸附试验(ELISA)等方法检测人类来源的Centriolin蛋白。Centriolin抗体(C-9)既可以以非共轭形式的抗Centriolin抗体提供,也可以多种共轭形式的抗Centriolin抗体提供,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®共轭物。细胞质分裂发生在有丝分裂的后期,描述了一个细胞的细胞质如何分裂以产生两个随后的子细胞的过程。Centriolin,也称为CEP110,是一种卷曲螺旋的中心体蛋白,对于中心体的成熟和正确功能至关重要。在细胞质分裂的最后一步--脱落过程中,Centriolin既协调又固定囊泡融合和囊泡外排复合物到子细胞的中体。Centriolin作为完成两个细胞分离所需的蛋白质复合物的锚点,启动并控制囊泡介导的细胞分裂。通过其发出脊椎动物细胞质分裂最后一步开始的信号的能力,Centriolin调节细胞增殖并有助于正确进入有丝分裂的S期。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Centriolin 抗体 (C-9) 参考文献:

  1. 在伴有t(8;9)(p12;q33)的8p12干细胞骨髓增生性疾病中,FGFR1与中心体相关蛋白CEP110融合。  |  Guasch, G., et al. 2000. Blood. 95: 1788-96. PMID: 10688839
  2. CEP110 和 ninein 位于中心体的一个特定区域,与中心体成熟有关。  |  Ou, YY., et al. 2002. J Cell Sci. 115: 1825-35. PMID: 11956314
  3. 细胞分裂的最后阶段和进入 S 期需要母细胞中心粒的一种新型人类蛋白质。  |  Gromley, A., et al. 2003. J Cell Biol. 161: 535-45. PMID: 12732615
  4. 对急性髓性白血病患者进行白血病相关抗原的体液检测。  |  Guinn, BA., et al. 2005. Biochem Biophys Res Commun. 335: 1293-304. PMID: 16112646
  5. 分泌泡介导的脱落需要外囊和 SNARE 复合物在中体的 Centriolin 锚定。  |  Gromley, A., et al. 2005. Cell. 123: 75-87. PMID: 16213214
  6. 脱落的环状模板。  |  von Dassow, G. and Bement, WM. 2005. Dev Cell. 9: 578-80. PMID: 16256733
  7. Hook2 定位于中心体,直接与 centriolin/CEP110 结合,有助于中心体功能的发挥。  |  Szebenyi, G., et al. 2007. Traffic. 8: 32-46. PMID: 17140400
  8. Hook2有助于凝集体的形成。  |  Szebenyi, G., et al. 2007. BMC Cell Biol. 8: 19. PMID: 17540036

订购信息

产品名称产品编号规格价格数量收藏夹

Centriolin 抗体 (C-9)

sc-365521
200 µg/ml
RMB2377.00

Centriolin (C-9): m-IgG Fc BP-HRP 套装

sc-529386
200 µg Ab; 10 µg BP
RMB2662.00

Centriolin (C-9): m-IgGκ BP-HRP 套装

sc-522263
200 µg Ab, 40 µg BP
RMB2662.00

Centriolin (C-9): m-IgG1 BP-HRP 套装

sc-543493
200 µg Ab; 20 µg BP
RMB2662.00

Centriolin 抗体 (C-9) AC

sc-365521 AC
500 µg/ml, 25% agarose
RMB3129.00

Centriolin 抗体 (C-9) HRP

sc-365521 HRP
200 µg/ml
RMB2377.00

Centriolin 抗体 (C-9) FITC

sc-365521 FITC
200 µg/ml
RMB2482.00

Centriolin 抗体 (C-9) PE

sc-365521 PE
200 µg/ml
RMB2580.00

Centriolin 抗体 (C-9) Alexa Fluor® 488

sc-365521 AF488
200 µg/ml
RMB2685.00

Centriolin 抗体 (C-9) Alexa Fluor® 546

sc-365521 AF546
200 µg/ml
RMB2685.00

Centriolin 抗体 (C-9) Alexa Fluor® 594

sc-365521 AF594
200 µg/ml
RMB2685.00

Centriolin 抗体 (C-9) Alexa Fluor® 647

sc-365521 AF647
200 µg/ml
RMB2685.00

Centriolin 抗体 (C-9) Alexa Fluor® 680

sc-365521 AF680
200 µg/ml
RMB2685.00

Centriolin 抗体 (C-9) Alexa Fluor® 790

sc-365521 AF790
200 µg/ml
RMB2685.00

in which cell type is expressed centriolin, acp1, sass6, dynactin (DCNT1) ? in Neuro2a??

Asked by: jack1111111111
Thank you for your question. Centriolin is widely expressed with highest levels in testis and trachea. We recommend the following positive controls: THP-1 cell lysate: sc-2238, SK-BR-3 cell lysate: sc-2218 or U-937 cell lysate: sc-2239.
Answered by: Tech Support Europe
Date published: 2021-06-25

I am using Centriolin (C-9): sc-365521 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great!I have used it for IF. The samples were fixed with MeOH for 5 minutes at -20°C. After blocking solution, I have used the diluited antibody (1:200) ON. Clear signal with no background!
Date published: 2020-02-25
Rated 5 out of 5 by from Excellent antibodyi used it for IF at 1:40 and WB 1:750 dilution using PFA-fixed MDCK cells
Date published: 2019-10-07
Rated 5 out of 5 by from Works well for IFI used it for immunofluorescence at 1:200 dilution. Stain centrosome well.
Date published: 2019-04-11
Rated 5 out of 5 by from a good antibody for both WB and IFThis antibody recognizes a clear band of predicted M.W. on WB (1:500 dilution, 40 ug HeLa extract). It also works for IF using formaldehyde-fixed HeLa cells (1:175 dilution) although with some background signal.
Date published: 2017-09-03
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic andExcellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-05-16
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Centriolin 抗体 (C-9) is rated 5.0 out of 5 by 6.
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