Date published: 2025-9-7
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Cdk2 Antibody (0.N.198) - Western Blotting - Image 376375
Cdk2 抗体 (0. N. 198): sc-70829. K-562 (A), JAR (B), SK-MEL-24 (C), F9 (D), c4 (E) 和 C6 (F) 全细胞裂解液中 Cdk2 表达的 Western 印迹分析.
Cdk2 抗体 (0. N. 198): sc-70829. K-562 (A), JAR (B), SK-MEL-24 (C), F9 (D), c4 (E) 和 C6 (F) 全细胞裂解液中 Cdk2 表达的 Western 印迹分析.

Cdk2 抗体 (0.N.198): sc-70829

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寫評論提問

说明书
  • Cdk2抗体(0.N.198)是小鼠单克隆IgG2a κ, 在8篇文献中引用，规格为200 µg/ml
  • 针对human重组Cdk2
  • 推荐用于 mouse, rat 和 human 来源的Cdk2 WB 和 IP检测
  • 看 Cdk2 (D-12): sc-6248，可查看有标记的 Cdk2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 2a BP-HRP">m-IgG2a BP-HRP是Cdk2 Antibody (0.N.198) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Cdk2 Antibody (0.N.198) 打包提供（请参阅下面的订购信息）。

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基因信息
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研究信息

関連項目

额外Cdk抗体，包括Cdk1, Cdk11, Cdk2, Cdk3, Cdk4, Cdk5, Cdk6, Cdk7, Cdk8和Cdk9

在脊椎动物中，就像在酵母中一样，已经发现了多种细胞周期蛋白，包括哺乳动物中总共八种这样的调节蛋白。与酵母中的情况不同，Cdc2 p34激酶并不是脊椎动物中唯一能够与细胞周期蛋白相互作用的催化亚基。尽管Cdc2 p34对于脊椎动物细胞从G2期到M期的过渡至关重要，但第二种与Cdc2相关的激酶也参与细胞周期调控。这种被指定为细胞周期蛋白依赖性激酶2（Cdk2）p33的蛋白质也能与细胞周期蛋白结合，其激酶活性在细胞周期中受到时间调控。已经发现了另外几种与Cdc2 p34相关的细胞周期蛋白依赖性激酶，包括Cdk3-Cdk8、PCTAIRE-1-3和KKIALRE。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Cdk2 抗体 (0.N.198)

sc-70829
200 µg/ml
RMB2377.00

Cdk2 (0.N.198): m-IgG2a BP-HRP 套装

sc-546004
200 µg Ab; 10 µg BP
RMB2662.00

CDK2的稀释倍数是多少啊

Asked by: 奔跑的小龙
感谢您的提问。免疫印迹 (起始稀释度 1:200, 稀释范围 1:100-1:1000) ，免疫沉淀[100-500 µg 总蛋白 (1 ml 细胞裂解液) 中加1-2 µg抗体]. Thank you for your question. Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) and immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)]
Answered by: Technical Support 8
Date published: 2018-12-21

How much sample do you recommend loading on the gel for Western blot detection using Cdk2 (0.N.198): sc-70829?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from CleanThe western blot effect is good antibody, is very clean, we are very satisfied.
Date published: 2017-04-10
Rated 4 out of 5 by from Good WB publishable data gathered with human cellsGood WBand IF publishable data gathered with 3T3 cells and F9 cells
Date published: 2017-03-28
Rated 4 out of 5 by from Good WB publishable data gathered with human cellsGood WB publishable data gathered with human cells. -SCBT Publication Review
Date published: 2015-04-13
Rated 3 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2013-10-30
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Cdk2 抗体 (0.N.198) is rated 4.0 out of 5 by 4.
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