Date published: 2025-9-7
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Cdc42 Antibody (B-8) - Western Blotting - Image 352730
Cdc42 抗体 (B-8): sc-8401. 3611-RF (A), Hep G2 (B), NIH/3T3 (C), RAW 309 Cr. 1 (D) 和 MCF7 (E) 全细胞裂解液中 Cdc42 表达的 Western 印迹分析.
Cdc42 抗体 (B-8): sc-8401. 3611-RF (A), Hep G2 (B), NIH/3T3 (C), RAW 309 Cr. 1 (D) 和 MCF7 (E) 全细胞裂解液中 Cdc42 表达的 Western 印迹分析.

Cdc42 抗体 (B-8): sc-8401

4.5(36)
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说明书
  • Cdc42 抗体 B-8 是小鼠单克隆 IgG3 κ，Cdc42抗体, 在183篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Cdc42的C-terminus的氨基酸166-182之间
  • 推荐WB, IP, IF, IHC(P) 和 ELISA检测mouse, rat, human, Drosophila 和 Xenopus来源的Cdc42; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取Cdc42 (B-8): sc-8401的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP和m-IgGκ BP-HRP是Cdc42抗体（B-8）在WB and IHC(P)应用中的首选二次检测试剂。这些试剂现在与Cdc42 抗体（B-8）捆绑销售（见下文订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cdc 抗体 包括 Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26和Cdc27

Cdc42抗体（B-8）是一种小鼠单克隆IgG3 kappa轻链抗体，用于检测Cdc42，也称为细胞分裂控制蛋白42同源物或G25K GTP结合蛋白。Anti-Cdc42抗体（B-8）靶向人类Cdc42 C末端166-182氨基酸的精确表位。Cdc42是Rho家族GTP酶中的关键成员，属于更大的Ras超家族，在调节细胞形态、迁移、细胞周期进展和细胞骨架动力学等各种细胞过程中发挥着关键作用。这些功能对于正常的细胞运作和发育至关重要，而Cdc42的失调与包括癌症、神经退行性疾病和发育障碍在内的多种病理状况有关，这凸显了其作为治疗靶点和研究重点的重要性。Cdc42单克隆抗体（B-8）具有广泛的物种反应性，可有效识别小鼠、大鼠、人、果蝇、非洲爪蟾以及马、犬、牛、猪和鸟类等其他物种的Cdc42蛋白，使其成为多种实验应用的多功能工具。Anti-Cdc42抗体（B-8）适用于多种技术，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA），有助于对Cdc42的表达和功能进行全面研究。Cdc42 (B-8) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，可满足各种研究需求。通过利用Cdc42单克隆抗体（B-8），研究人员可以有效地研究Cdc42介导的信号传导通路和分子机制，从而加深我们对细胞生长、分裂和相关疾病根本原因的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc42 抗体 (B-8) 参考文献:

  1. dbl 癌基因产物对 CDC42Hs 蛋白上鸟嘌呤核苷酸交换的催化作用。  |  Hart, MJ., et al. 1991. Nature. 354: 311-4. PMID: 1956381
  2. 小 GTP 结合蛋白的细胞功能。  |  Hall, A. 1990. Science. 249: 635-40. PMID: 2116664
  3. 酵母细胞周期基因 CDC42 的人类同源物 G25K cDNA 的分子克隆和表达。  |  Munemitsu, S., et al. 1990. Mol Cell Biol. 10: 5977-82. PMID: 2122236
  4. GTPase 超家族：多种细胞功能的保守开关。  |  Bourne, HR., et al. 1990. Nature. 348: 125-32. PMID: 2122258
  5. 人胎盘 GTP 结合蛋白 Gp（G25K）基因的分子克隆：确定该 GTP 结合蛋白为酵母细胞分裂周期蛋白 CDC42 的人类同源物。  |  Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
  6. 参与细胞极性发育的酿酒酵母基因 CDC42 的分子特征。  |  Johnson, DI. and Pringle, JR. 1990. J Cell Biol. 111: 143-52. PMID: 2164028
  7. CDC42 和 CDC43，这是另外两个参与酵母出芽和建立细胞极性的基因。  |  Adams, AE., et al. 1990. J Cell Biol. 111: 131-42. PMID: 2195038
  8. 从人类胎盘膜中纯化主要的 GTP 结合蛋白。  |  Evans, T., et al. 1986. J Biol Chem. 261: 7052-9. PMID: 3084494

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc42 抗体 (B-8)

sc-8401
200 µg/ml
$316.00

Cdc42 (B-8): m-IgGκ BP-HRP 套装

sc-520547
200 µg Ab, 40 µg BP
$354.00

Cdc42 (B-8): m-IgG3 BP-HRP 套装

sc-550330
200 µg Ab; 40 µg BP
$354.00

Cdc42 抗体 (B-8) AC

sc-8401 AC
500 µg/ml, 25% agarose
$416.00

Cdc42 抗体 (B-8) Alexa Fluor® 488

sc-8401 AF488
200 µg/ml
$357.00

Cdc42 抗体 (B-8) Alexa Fluor® 647

sc-8401 AF647
200 µg/ml
$357.00

Cdc42 抗体 (B-8) FITC

sc-8401 FITC
200 µg/ml
$330.00

Cdc42 抗体 (B-8) HRP

sc-8401 HRP
200 µg/ml
$316.00

Cdc42 (B-8) 中和勝肽

sc-8401 P
100 µg/0.5 ml
$68.00

Cdc42 抗体 (B-8) PE

sc-8401 PE
200 µg/ml
$343.00

Could I receive a test sample of your anti Cdc42 (SC-8401) antibody?

Asked by: Timothy
Thank you for your question. Yes, for a limited time we are offering a free 10 µg sample of this mouse monoclonal antibody, Cdc42 (B-8): sc-8401. Please contact our Technical Service Department to request a sample. Please be prepared to provide your complete shipping address and contact phone number for your lab. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-06-06

I am going to be staining some paraffin embedded sections. What is the recommended secondary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-23
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Rated 5 out of 5 by from works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:500 for overnight at 4oC
Date published: 2024-12-13
Rated 4 out of 5 by from Funziona beneab I 1:1000 BSA 5% e ab II 1:3000 anti mouse 3%milk
Date published: 2021-10-11
Rated 4 out of 5 by from It works well in western blottingTested in whole cell lysate of glioblastoma primary cells and U-87MG cells (30ug of protein), antibody dilution 1:500 in TBS-T 0.1%.
Date published: 2020-03-25
Rated 3 out of 5 by from Works for western blotsWorks well for western blots. Did not work for immunoflourescence in our hands, may require standarization
Date published: 2019-03-18
Rated 4 out of 5 by from Good antibodyIt detects well cdc42 in western blots. It is better to use PVDF membrane for blotting.
Date published: 2019-02-26
Rated 5 out of 5 by from Worked well for immunoblotI used this antibody to stain my membrane for CDC42 in iPSC derived neurons plated for two months.
Date published: 2018-04-24
Rated 4 out of 5 by from specific, but weakThe antibody is rather clean with low background in non-milk block. But it is either low titer or low affinity, the signal is very weak even at 1:100.
Date published: 2018-04-13
Rated 5 out of 5 by from Great product!I received this Ab in a couple of months ago and was glad to see it worked nicely! Will definitely purchase when we need it...
Date published: 2018-01-13
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Cdc42 抗体 (B-8) is rated 4.5 out of 5 by 36.
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