Date published: 2025-9-7

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Cdc27 抗体 (AF3.1): sc-9972

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说明书
  • Cdc27 抗体 AF3.1 是小鼠单克隆 IgG2b κ,Cdc27抗体, 在73篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Cdc27的氨基酸814-823
  • Cdc27 抗体 (AF3.1) 推荐用于WB, IP, IF 和 IHC(P) ,检测 mouse, rat, human 和 Xenopus来源的Cdc27; 也和以下物种反应,包括: 和 equine and canine
  • 抗Cdc27抗体(AF3.1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cdc27 (AF3.1): sc-9972免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Cdc27 抗体 (AF3.1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc27 抗体 (AF3.1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Cdc27抗体(AF3.1)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学(IHC)检测小鼠、大鼠、人类和非洲爪蟾的Cdc27蛋白。Cdc27 单克隆抗体(AF3.1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。Cdc27在细胞周期的调节中起着至关重要的作用,尤其是作为后期促进复合物(APC)的组成部分,它对于细胞周期蛋白和其他调节蛋白的及时降解至关重要。这种降解对于细胞周期不同阶段之间的转换至关重要,可确保细胞在适当的时间正确分裂。通过促进细胞周期蛋白B的泛素化,Cdc27有助于触发中期-后期过渡,从而防止染色体分离错误,避免非整倍性或其他细胞周期相关疾病。因此,Cdc27和APC的正常运作对于维持基因组稳定性和防止肿瘤发生至关重要,这使得Cdc27(AF3.1)抗体成为研究人员研究细胞周期调节及其在癌症生物学中的影响的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc27 抗体 (AF3.1) 参考文献:

  1. 消耗布氏锥虫的无性繁殖促进复合体或环体(APC/C)亚基同源物 APC1 或 CDC27 可使原环体停滞于分裂期,但血流体停滞于无性期。  |  Kumar, P. and Wang, CC. 2005. J Biol Chem. 280: 31783-91. PMID: 15994309
  2. RNA 干扰敲除 hU2AF35 会影响细胞周期的进展,并调节 Cdc25 转录本的替代剪接。  |  Pacheco, TR., et al. 2006. Mol Biol Cell. 17: 4187-99. PMID: 16855028
  3. cdc25 是一种特异性酪氨酸磷酸酶,可直接激活 p34cdc2。  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
  4. 无性繁殖促进复合体/Cdc27 的磷酸化参与了 TGF-beta 信号传导。  |  Zhang, L., et al. 2011. J Biol Chem. 286: 10041-50. PMID: 21209074
  5. 酵母(Saccharomyces cerevisiae)cdc突变体中人工染色体的有丝分裂传递。  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
  6. 鉴定 Elmo1 和 Cdc27 之间的新型蛋白质相互作用。  |  Lee, J., et al. 2016. Biochem Biophys Res Commun. 471: 497-502. PMID: 26882976
  7. CDC27 通过促进上皮向间质转化诱导结直肠癌的转移和侵袭  |  Qiu, L., et al. 2017. J Cancer. 8: 2626-2635. PMID: 28900500
  8. 细胞周期的新转折  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
  9. 含有 CDC27 和 CDC16 的 20S 复合物可催化泛素与细胞周期蛋白 B 的有丝分裂特异性连接。  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
  10. 通过酵母互补克隆出 CDC34 细胞周期基因的人类同源物。  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
  11. 哺乳动物的 p50Cdc37 是 Hsp90 的蛋白激酶靶向亚基,能与 Cdk4 结合并使其稳定。  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
  12. 一种与酵母 Cdc6p 相关的人类蛋白质。  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc27 抗体 (AF3.1)

sc-9972
200 µg/ml
RMB2377.00

Cdc27 (AF3.1): m-IgGκ BP-HRP 套装

sc-520568
200 µg Ab, 40 µg BP
RMB2662.00

Cdc27 (AF3.1): m-IgG2b BP-HRP 套装

sc-548836
200 µg Ab; 10 µg BP
RMB2662.00

Cdc27 抗体 (AF3.1) AC

sc-9972 AC
500 µg/ml, 25% agarose
RMB3129.00

Cdc27 抗体 (AF3.1) HRP

sc-9972 HRP
200 µg/ml
RMB2377.00

Cdc27 抗体 (AF3.1) FITC

sc-9972 FITC
200 µg/ml
RMB2482.00

Cdc27 抗体 (AF3.1) PE

sc-9972 PE
200 µg/ml
RMB2580.00

Cdc27 抗体 (AF3.1) Alexa Fluor® 488

sc-9972 AF488
200 µg/ml
RMB2685.00

Cdc27 抗体 (AF3.1) Alexa Fluor® 546

sc-9972 AF546
200 µg/ml
RMB2685.00

Cdc27 抗体 (AF3.1) Alexa Fluor® 594

sc-9972 AF594
200 µg/ml
RMB2685.00

Cdc27 抗体 (AF3.1) Alexa Fluor® 647

sc-9972 AF647
200 µg/ml
RMB2685.00

Cdc27 抗体 (AF3.1) Alexa Fluor® 680

sc-9972 AF680
200 µg/ml
RMB2685.00

Cdc27 抗体 (AF3.1) Alexa Fluor® 790

sc-9972 AF790
200 µg/ml
RMB2685.00

Can you recommend a positive control to use with Cdc27 (AF3.1): sc-9972 for Western blot?

Asked by: jenni
We recommend K-562 (sc-2203), RAW 264.7 (sc-2211) or HeLa (sc-2200) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rab 7 in these cell lines.
Answered by: Technical Support 13
Date published: 2017-01-20

How can Cdc27 (AF3.1): sc-9972 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Cdc27 (AF3.1): sc-9972 monoclonal antibody is currently available conjugated to phycoerythrin (sc-9972 PE), fluorescein (sc-9972 FITC), Alexa Fluor® 488 (sc-9972 AF488) or Alexa Fluor® 647 (sc-9972 AF647)
Answered by: Technical Support 2
Date published: 2017-01-16
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Rated 5 out of 5 by from good productIt is a good antibody and works well for western blot.
Date published: 2019-10-28
Rated 5 out of 5 by from good productIt is a good antibody and works well for western blot.
Date published: 2018-08-22
Rated 5 out of 5 by from OutstandingVery strong western blot data; also superb IF and IHC staining
Date published: 2017-01-25
Rated 5 out of 5 by from Great IF stainingWe used this antibody for IF staining and got great images and nice intensity form HeLa cells nucleus
Date published: 2017-01-18
Rated 5 out of 5 by from Works well in IP & WBWorks well in IP & WB in detecting endogenous CDC27 in HeLa cells. The rest of the APC/C co-IPs with CDC27.
Date published: 2016-12-06
Rated 5 out of 5 by from This antibody worked well in out blot inThis antibody worked well in out blot in HeLa cells.
Date published: 2015-06-05
Rated 5 out of 5 by from Good WB antibody in rat cerebellar granule neuronsGood WB antibody in rat cerebellar granule neurons. -SCBT Publication Review
Date published: 2015-04-27
Rated 5 out of 5 by from Worked well in WB in human transfected HeLa cellsWorked well in WB in human transfected HeLa cells. -SCBT Publication Review
Date published: 2015-04-15
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Cdc27 抗体 (AF3.1) is rated 5.0 out of 5 by 11.
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